NOX5 (NM_001184779) Human Tagged ORF Clone

CAT#: RC230471

NOX5 (Myc-DDK-tagged)-Human NADPH oxidase, EF-hand calcium binding domain 5 (NOX5), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NOX5
• Synonyms: MGC149776; MGC149777
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230471 representing NM_001184779
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACACATCTGGAGACCCAGCCCAGACGGGCCCTGAAGGCTGTAGAGGCACCATGAGTGCCGAGGAGG
  ATGCCAGGTGGCTCCGGTGGGTGACTCAGCAGTTTAAGACCATTGCAGGAGAAGATGGGGAGATCAGCCT
  GCAAGAATTCAAAGCAGCTCTGCATGTGAAAGAGTCCTTCTTTGCAGAGCGATTCTTTGCCCTATTTGAC
  TCCGATAGAAGTGGCACCATCACCCTCCAGGAGCTGCAGGAGGCACTGACCCTGCTCATCCATGGCAGCC
  CCATGGACAAACTCAAATTCCTCTTCCAGGTGTATGACATCGATGGCAGTGGCTCCATTGACCCGGATGA
  GCTGCGCACTGTGCTGCAGTCGTGTCTGCGCGAGAGCGCCATCTCGCTGCCTGACGAGAAGCTGGACCAG
  CTGACGCTGGCGCTCTTCGAATCGGCCGACGCGGACGGCAACGGGGCCATCACCTTCGAGGAGCTCCGGG
  ACGAGCTGCAGCGCTTCCCCGGAGTCATGGAGAACCTGACCATCAGCGCTGCCCACTGGCTGACGGCCCC
  CGCCCCCCGCCCACGCCCGCGCCGGCCGCGCCAGCTGACCCGCGCCTACTGGCACAACCACCGCAGCCAG
  CTGTTCTGCCTGGCCACCTATGCAGGCCTCCACGTGCTGCTCTTCGGGCTGGCGGCCAGCGCGCACCGGG
  ACCTCGGCGCCAGCGTCATGGTGGCCAAGGGCTGCGGCCAGTGCCTCAACTTCGACTGCAGCTTCATCGC
  GGTGCTGATGCTCAGACGCTGCCTCACCTGGCTGCGGGCCACGTGGCTGGCTCAAGTCCTACCACTGGAC
  CAGAACATCCAGTTCCACCAGCTTATGGGCTACGTGGTAGTGGGGCTGTCCCTCGTGCACACCGTGGCTC
  ACACTGTGAACTTTGTACTCCAGGCTCAGGCGGAGGCCAGCCCTTTCCAGTTCTGGGAGCTGCTGCTCAC
  CACGAGGCCTGGCATTGGCTGGGTACACGGTTCGGCCTCCCCGACAGGTGTCGCTCTGCTGCTGCTGCTC
  CTCCTCATGTTCATCTGCTCCAGTTCCTGCATCCGCAGGAGTGGCCACTTTGAGGTGTTCTATTGGACTC
  ACCTGTCCTACCTCCTCGTGTGGCTTCTGCTCATCTTTCATGGGCCCAACTTCTGGAAGTGGCTGCTGGT
  GCCTGGAATCTTGTTTTTCCTGGAGAAGGCCATCGGACTGGCAGTGTCCCGCATGGCAGCCGTGTGCATC
  ATGGAAGTCAACCTCCTCCCCTCCAAGGTCACTCATCTCCTCATCAAGCGGCCCCCTTTTTTTCACTATA
  GACCTGGTGACTACTTGTATCTGAACATCCCCACCATTGCTCGCTATGAGTGGCACCCCTTCACCATCAG
  CAGTGCTCCTGAGCAGAAAGACACTATCTGGCTGCACATTCGGTCCCAAGGCCAGTGGACAAACAGGCTG
  TATGAGTCCTTCAAGGCATCAGACCCACTGGGCCGTGGTTCTAAGAGGCTGTCGAGGAGTGTGACAATGA
  GAAAGAGTCAAAGGTCGTCCAAGGGCTCTGAGATACTTTTGGAGAAACACAAATTCTGTAACATCAAGTG
  CTACATCGATGGGCCTTATGGGACCCCCACCCGCAGGATCTTTGCCTCTGAGCATGCCGTGCTCATCGGG
  GCAGGCATCGGCATCACCCCCTTTGCTTCCATTCTGCAGAGTATCATGTACAGGCACCAGAAAAGAAAGC
  ATACTTGCCCCAGCTGCCAGCACTCCTGGATCGAAGGTGTCCAAGACAACATGAAGCTCCATAAGGTGGA
  CTTTATCTGGATCAACAGAGACCAGCGGTCTTTCGAGTGGTTTGTGAGCCTGCTGACTAAACTGGAGATG
  GACCAGGCCGAGGAGGCTCAATACGGCCGCTTCCTGGAGCTGCATATGTACATGACATCTGCACTGGGCA
  AGAATGACATGAAGGCCATTGGCCTGCAGATGGCCCTTGACCTCCTGGCCAACAAGGAGAAGAAAGACTC
  CATCACGGGGCTGCAGACGCGCACCCAGCCTGGGCGGCCTGACTGGAGCAAGGTGTTCCAGAAAGTGGCT
  GCTGAGAAGAAGGGCAAGGTGCAGGTCTTCTTCTGTGGCTCCCCAGCTCTGGCCAAGGTGCTGAAGGGCC
  ATTGTGAGAAGTTCGGCTTCAGATTTTTCCAAGAGAATTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230471 representing NM_001184779
  Red=Cloning site Green=Tags(s)
  
  MNTSGDPAQTGPEGCRGTMSAEEDARWLRWVTQQFKTIAGEDGEISLQEFKAALHVKESFFAERFFALFD
  SDRSGTITLQELQEALTLLIHGSPMDKLKFLFQVYDIDGSGSIDPDELRTVLQSCLRESAISLPDEKLDQ
  LTLALFESADADGNGAITFEELRDELQRFPGVMENLTISAAHWLTAPAPRPRPRRPRQLTRAYWHNHRSQ
  LFCLATYAGLHVLLFGLAASAHRDLGASVMVAKGCGQCLNFDCSFIAVLMLRRCLTWLRATWLAQVLPLD
  QNIQFHQLMGYVVVGLSLVHTVAHTVNFVLQAQAEASPFQFWELLLTTRPGIGWVHGSASPTGVALLLLL
  LLMFICSSSCIRRSGHFEVFYWTHLSYLLVWLLLIFHGPNFWKWLLVPGILFFLEKAIGLAVSRMAAVCI
  MEVNLLPSKVTHLLIKRPPFFHYRPGDYLYLNIPTIARYEWHPFTISSAPEQKDTIWLHIRSQGQWTNRL
  YESFKASDPLGRGSKRLSRSVTMRKSQRSSKGSEILLEKHKFCNIKCYIDGPYGTPTRRIFASEHAVLIG
  AGIGITPFASILQSIMYRHQKRKHTCPSCQHSWIEGVQDNMKLHKVDFIWINRDQRSFEWFVSLLTKLEM
  DQAEEAQYGRFLELHMYMTSALGKNDMKAIGLQMALDLLANKEKKDSITGLQTRTQPGRPDWSKVFQKVA
  AEKKGKVQVFFCGSPALAKVLKGHCEKFGFRFFQENF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184779
• ORF Size: 2211 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001184779.1, NP_001171708.1
• RefSeq ORF: 2214
• Locus ID: 79400
• Protein Families: Ion Channels: Other, Transmembrane
• MW: 84.2 kDa
• Gene Summary: This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]