STIM2 (NM_001169118) Human Tagged ORF Clone

CAT#: RC230479

STIM2 (Myc-DDK-tagged)-Human stromal interaction molecule 2 (STIM2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STIM2
• Synonyms: FLJ39527; KIAA1482
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230479 representing NM_001169118
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  TTGCTGGTGCTCGGGCTGCTGGTAGCCGGAGCGGCGGACGGATGCGAGCTTGTGCCCCGGCACCTCCGCG
  GGCGGCGGGCGACTGGCTCTGCCGCAACTGCCGCCTCCTCTCCCGCCGCGGCGGCCGGCGATAGCCCGGC
  GCTCATGACAGATCCCTGCATGTCACTGAGTCCACCATGCTTTACAGAAGAAGACAGATTTAGTCTGGAA
  GCTCTTCAAACAATACATAAACAAATGGATGATGACAAAGATGGTGGAATTGAAGTAGAGGAAAGTGATG
  AATTCATCAGAGAAGATATGAAATATAAAGATGCTACTAATAAACACAGCCATCTGCACAGAGAAGATAA
  ACATATAACGATTGAGGATTTATGGAAACGATGGAAAACATCAGAAGTTCATAATTGGACCCTTGAAGAC
  ACTCTTCAGTGGTTGATAGAGTTTGTTGAACTACCCCAATATGAGAAGAATTTTAGAGACAACAATGTCA
  AAGGAACGACACTTCCCAGGATAGCAGTGCACGAACCTTCATTTATGATCTCCCAGTTGAAAATCAGTGA
  CCGGAGTCACAGACAAAAACTTCAGCTCAAGGCATTGGATGTGGTTTTGTTTGGACCTCTAACACGCCCA
  CCTCATAACTGGATGAAAGATTTTATCCTCACAGTTTCTATAGTAATTGGTGTTGGAGGCTGCTGGTTTG
  CTTATACGCAGAATAAGACATCAAAAGAACATGTTGCAAAAATGATGAAAGATTTAGAGAGCTTACAAAC
  TGCAGAGCAAAGTCTAATGGACTTACAAGAGAGGCTTGAAAAGGCACAGGAAGAAAACAGAAATGTTGCT
  GTAGAAAAGCAAAATTTAGAGCGCAAAATGATGGATGAAATCAATTATGCAAAGGAGGAGGCTTGTCGGC
  TGAGAGAGCTAAGGGAGGGAGCTGAATGTGAATTGAGTAGACGTCAGTATGCAGAACAGGAATTGGAACA
  GGTTCGCATGGCTCTGAAAAAGGCCGAAAAAGAATTTGAACTGAGAAGCAGTTGGTCTGTTCCAGATGCA
  CTTCAGAAATGGCTTCAGTTAACACATGAAGTAGAAGTGCAATACTACAATATTAAAAGACAAAACGCTG
  AAATGCAGCTAGCTATTGCTAAAGATGAGGTTGCTGCTTCATATCTGATTCAGGCAGAAAAAATTAAAAA
  GAAGAGAAGCACAGTCTTTGGGACTCTGCACGTTGCACACAGCTCCTCCCTAGATGAGGTAGACCACAAA
  ATTCTGGAAGCAAAGAAAGCTCTCTCTGAGTTGACAACTTGTTTACGAGAACGACTTTTTCGCTGGCAAC
  AAATTGAGAAGATCTGTGGCTTTCAGATAGCCCATAACTCAGGACTCCCCAGCCTGACCTCTTCCCTTTA
  TTCTGATCACAGCTGGGTGGTGATGCCCAGAGTCTCCATTCCACCCTATCCAATTGCTGGAGGAGTTGAT
  GACTTAGATGAAGACACACCCCCAATAGTGTCACAATTTCCCGGGACCATGGCTAAACCTCCTGGATCAT
  TAGCCAGAAGCAGCAGCCTGTGCCGTTCACGCCGCAGCATTGTGCCGTCCTCGCCTCAGCCTCAGCGAGC
  TCAGCTTGCTCCACACGCCCCCCACCCGTCACACCCTCGGCACCCTCACCACCCGCAACACACACCACAC
  TCCTTGCCTTCCCCTGATCCAGATATCCTCTCAGTGTCAAGTTGCCCTGCGCTTTATCGAAATGAAGAGG
  AGGAAGAGGCCATTTACTTCTCTGCTGAAAAGCAATGGGAAGTGCCAGACACAGCTTCAGAATGTGACTC
  CTTAAATTCTTCCATTGGAAGGAAACAGTCTCCTCCTTTAAGCCTCGAGATATACCAAACATTATCTCCG
  CGAAAGATATCAAGAGATGAGGTGTCCCTAGAGGATTCCTCCCGAGGGGATTCGCCTGTAACTGTGGATG
  TGTCTTGGGGTTCTCCCGACTGTGTAGGTCTGACAGAAACTAAGAGTATGATCTTCAGTCCTGCAAGCAA
  AGTGTACAATGGCATTTTGGAGAAATCCTGTAGCATGAACCAGCTTTCCAGTGGCATCCCGGTGCCTAAA
  CCTCGCCACACATCATGTTCCTCAGCTGGCAACGACAGTAAACCAGTTCAGGAAGCCCCAAGTGTTGCCA
  GAATAAGCAGCATCCCACATGACCTTTGTCATAATGGAGAGAAAAGCAAAAAGCCATCAAAAATCAAAAG
  CCTTTTTAAGAAGAAATCTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230479 representing NM_001169118
  Red=Cloning site Green=Tags(s)
  
  LLVLGLLVAGAADGCELVPRHLRGRRATGSAATAASSPAAAAGDSPALMTDPCMSLSPPCFTEEDRFSLE
  ALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIEDLWKRWKTSEVHNWTLED
  TLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRP
  PHNWMKDFILTVSIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEENRNVA
  VEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKKAEKEFELRSSWSVPDA
  LQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEVAASYLIQAEKIKKKRSTVFGTLHVAHSSSLDEVDHK
  ILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSLYSDHSWVVMPRVSIPPYPIAGGVD
  DLDEDTPPIVSQFPGTMAKPPGSLARSSSLCRSRRSIVPSSPQPQRAQLAPHAPHPSHPRHPHHPQHTPH
  SLPSPDPDILSVSSCPALYRNEEEEEAIYFSAEKQWEVPDTASECDSLNSSIGRKQSPPLSLEIYQTLSP
  RKISRDEVSLEDSSRGDSPVTVDVSWGSPDCVGLTETKSMIFSPASKVYNGILEKSCSMNQLSSGIPVPK
  PRHTSCSSAGNDSKPVQEAPSVARISSIPHDLCHNGEKSKKPSKIKSLFKKKSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001169118
• ORF Size: 2262 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001169118.1, NP_001162589.1
• RefSeq ORF: 2265
• Locus ID: 57620
• MW: 85.2 kDa
• Gene Summary: This gene is a member of the stromal interaction molecule (STIM) family and likely arose, along with related family member STIM1, from a common ancestral gene. The encoded protein functions to regulate calcium concentrations in the cytosol and endoplasmic reticulum, and is involved in the activation of plasma membrane Orai Ca(2+) entry channels. This gene initiates translation from a non-AUG (UUG) start site. A signal peptide is cleaved from the resulting protein. Multiple transcript variants result from alternative splicing. [provided by RefSeq, Dec 2009]