RIM1 (RIMS1) (NM_001168409) Human Tagged ORF Clone

CAT#: RC230481

RIMS1 (Myc-DDK-tagged)-Human regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RIMS1
• Synonyms: CORD7; RAB3IP2; RIM; RIM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230481 representing NM_001168409
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAAAGACTCAGGTGCATTGCTGGGTCTGAAAGTTGTTGGAGGAAAAATGACTGACTTAGGACGAC
  TTGGTGCTTTCATCACCAAAGTAAAGAAGGGTAGCCTAGCAGATGTAGTTGGACACCTAAGAGCAGGGGA
  TGAAGTTCTAGAATGGAATGGTAAACCCCTGCCGGGAGCTACAAATGAAGAAGTTTACAACATTATTTTA
  GAATCAAAATCAGAACCTCAAGTTGAAATTATTGTTTCAAGGCCTATTGGTGACATTCCCCGGATTCCTG
  AGAGCTCCCACCCTCCACTGGAGTCCAGTTCAAGTTCCTTTGAATCTCAGAAGATGGAAAGGCCTTCCAT
  TTCTGTTATTTCTCCAACAAGTCCTGGAGCTCTAAAAGATGCCCCACAAGTCTTACCAGGGCAACTTTCT
  GTGAAGTTGTGGTATGATAAAGTGGGACACCAGCTGATTGTAAATGTTCTGCAAGCAACAGATCTACCTG
  CTAGAGTAGATGGACGTCCTCGAAATCCCTATGTAAAAATGTATTTTCTTCCAGATAGAAGTGATAAAAG
  TAAAAGGAGGACCAAAACAGTAAAGAAAATACTAGAACCAAAATGGAATCAAACTTTTGTCTATTCACAT
  GTACATCGTAGAGATTTTAGAGAACGAATGTTAGAAATAACTGTGTGGGACCAACCAAGAGTGCAAGAAG
  AAGAAAGTGAATTTCTTGGAGAGATCCTCATAGAATTGGAGACAGCGCTTTTAGATGATGAACCGCATTG
  GTATAAACTTCAGACACATGATGAGTCTTCACTACCTCTGCCTCAGCCATCACCTTTCATGCCAAGGCGA
  CATATTCATGGAGAAAGCTCTAGCAAAAAGCTACAAAGATCTCAGCGAATCAGTGATAGTGACATCTCAG
  ATTATGAGGTTGATGATGGTATTGGCGTAGTTCCTCCAGTAGGCTATAGGTCTAGTGCTAGAGAAAGTAA
  ATCTACAACATTAACTGTGCCAGAACAGCAAAGAACAACTCATCACCGCTCACGTTCAGTATCTCCTCAT
  CGCGGCAATGATCAGGGAAAGCCGCGTTCACGTTTACCAAATGTGCCATTACAGAGGAGTTTAGATGAAA
  TTCATCCAACAAGAAGGTCACGTTCTCCAACCAGACACCATGATGCCTCCCGAAGTCCAGTTGATCATAG
  AACCAGAGATGTGGATAGTCAGTATTTATCAGAACAAGACAGTGAGCTTCTTATGCTGCCCAGAGCAAAA
  CGAGGACGAAGTGCAGAATGCCTACATACTACCAGTGAACTGCAGCCCTTTCTTGACAGGGCTAGGAGTG
  CTAGTACCAACTGCTTGAGACCAGATACTAGTTTGCATTCACCAGAACGAGAAAGGCACTCCAGAAAGTC
  TGAAAGATCTAGCATCCAAAAACAGACTAGGAAAGGCACTGCCTCTGATGCAGAAAGAATGCACCGACAG
  AGAAGTCCAACACAATCTCCTCCAGCAGACACATCGTTCAGCAGTCGCAGGGGAAGACAGCTCCCACAAG
  TGCCAGTGAGAAGCGGCAGTATAGAACAAGAGTCGGGCCACAAAAAGTTAAAAAGTACCATCCAGAGAAG
  CACAGAAACAGGCATGGCAGCTGAAATGAGAAAGATGGTAAGGCAGCCGAGCCGAGAGTCTACTGATGGC
  AGCATCAACAGTTACAGCTCTGAGGGCAATTTAATATTTCCTGGAGTGCGACTGGGAGCTGACAGTCAAT
  TCAGTGATTTTCTTGATGGATTGGGACCAGCCCAGCTTGTTGGCCGCCAAACCCTTGCCACCCCTGCAAT
  GGGTGATATACAAATAGGAATGGAGGACAAAAAGGGCCAATTAGAAGTGGAAGTCATTAGAGCACGAAGC
  CTCACACAAAAGCCTGGTTCCAAATCTACACCTGCTCCATATGTCAAAGTATATCTTTTGGAAAATGGGG
  CCTGTATAGCCAAGAAGAAGACAAGAATTGCACGAAAAACCCTTGATCCTTTGTATCAGCAGTCTCTGGT
  TTTTGATGAAAGTCCACAGGGTAAAGTTCTTCAGGTGATTGTCTGGGGAGACTATGGCAGAATGGACCAC
  AAATGCTTTATGGGTGTGGCTCAGATCTTGTTGGAAGAACTCGACCTGTCCAGCATGGTGATCGGATGGT
  ACAAATTGTTCCCACCGTCCTCACTGGTGGATCCCACACTCACTCCCCTCACCCGGCGGGCTTCCCAGTC
  ATCTCTGGAAAGTTCAACTGGGCCTCCCTGTATTCGATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230481 representing NM_001168409
  Red=Cloning site Green=Tags(s)
  
  MPKDSGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIIL
  ESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLS
  VKLWYDKVGHQLIVNVLQATDLPARVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKILEPKWNQTFVYSH
  VHRRDFRERMLEITVWDQPRVQEEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRR
  HIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRSSARESKSTTLTVPEQQRTTHHRSRSVSPH
  RGNDQGKPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPVDHRTRDVDSQYLSEQDSELLMLPRAK
  RGRSAECLHTTSELQPFLDRARSASTNCLRPDTSLHSPERERHSRKSERSSIQKQTRKGTASDAERMHRQ
  RSPTQSPPADTSFSSRRGRQLPQVPVRSGSIEQESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDG
  SINSYSSEGNLIFPGVRLGADSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARS
  LTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDH
  KCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLTPLTRRASQSSLESSTGPPCIRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168409
• ORF Size: 2280 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001168409.1, NP_001161881.1
• RefSeq ORF: 2283
• Locus ID: 22999
• MW: 85.6 kDa
• Gene Summary: The protein encoded by this gene is a RAS gene superfamily member that regulates synaptic vesicle exocytosis. This gene also plays a role in the regulation of voltage-gated calcium channels during neurotransmitter and insulin release. Mutations have suggested a role cognition and have been identified as the cause of cone-rod dystrophy type 7. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012]