RIM1 (RIMS1) (NM_001168410) Human Tagged ORF Clone

CAT#: RC230521

RIMS1 (Myc-DDK-tagged)-Human regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RIMS1
• Synonyms: CORD7; RAB3IP2; RIM; RIM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230521 representing NM_001168410
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGCTGGGTTTCTGCAGTTTCTACTACTTCATACATTGCACTCAGGAACAGGTGATTTGGATTATT
  ACTGGTTGGATCCTGCCACGTGGCACAGCCGGGAGACATCACCTATTAGTTCGCATCCTGTAACGTGGCA
  ACCATCTAAAGAGGGGGACCGATTAATTGGACGTGTTATTCTTAACAAGAGAACAACCATGCCCAAAGAC
  TCAGGTGCATTGCTGGGTCTGAAAGTTGTTGGAGGAAAAATGACTGACTTAGGACGACTTGGTGCTTTCA
  TCACCAAAGTAAAGAAGGGTAGCCTAGCAGATGTAGTTGGACACCTAAGAGCAGGGGATGAAGTTCTAGA
  ATGGAATGGTAAACCCCTGCCGGGAGCTACAAATGAAGAAGTTTACAACATTATTTTAGAATCAAAATCA
  GAACCTCAAGTTGAAATTATTGTTTCAAGGCCTATTGGTGACATTCCCCGGATTCCTGAGAGCTCCCACC
  CTCCACTGGAGTCCAGTTCAAGTTCCTTTGAATCTCAGAAGATGGAAAGGCCTTCCATTTCTGTTATTTC
  TCCAACAAGTCCTGGAGCTCTAAAAGATGCCCCACAAGTCTTACCAGGGCAACTTTCTGTGAAGTTGTGG
  TATGATAAAGTGGGACACCAGCTGATTGTAAATGTTCTGCAAGCAACAGATCTACCTGCTAGAGTAGATG
  GACGTCCTCGAAATCCCTATGTAAAAATGTATTTTCTTCCAGATAGAAGTGATAAAAGTAAAAGGAGGAC
  CAAAACAGTAAAGAAAATACTAGAACCAAAATGGAATCAAACTTTTGTCTATTCACATGTACATCGTAGA
  GATTTTAGAGAACGAATGTTAGAAATAACTGTGTGGGACCAACCAAGAGTGCAAGAAGAAGAAAGTGAAT
  TTCTTGGAGAGATCCTCATAGAATTGGAGACAGCGCTTTTAGATGATGAACCGCATTGGTATAAACTTCA
  GACACATGATGAGTCTTCACTACCTCTGCCTCAGCCATCACCTTTCATGCCAAGGCGACATATTCATGGA
  GAAAGCTCTAGCAAAAAGCTACAAAGATCTCAGCGAATCAGTGATAGTGACATCTCAGATTATGAGGTTG
  ATGATGGTATTGGCGTAGTTCCTCCAGTAGGCTATAGGTCTAGTGCTAGAGAAAGTAAATCTACAACATT
  AACTGTGCCAGAACAGCAAAGAACAACTCATCACCGCTCACGTTCAGTATCTCCTCATCGCGGCAATGAT
  CAGGGAAAGCCGCGTTCACGTTTACCAAATGTGCCATTACAGAGGAGTTTAGATGAAATTCATCCAACAA
  GAAGGTCACGTTCTCCAACCAGACACCATGATGCCTCCCGAAGTCCAGTTGATCATAGAACCAGAGATGT
  GGATAGTCAGTATTTATCAGAACAAGACAGTGAGCTTCTTATGCTGCCCAGAGCAAAACGAGGACGAAGT
  GCAGAATGCCTACATACTACCAGTGAACTGCAGCCCTTTCTTGACAGGGCTAGGAGTGCTAGTACCAACT
  GCTTGAGACCAGATACTAGTTTGCATTCACCAGAACGAGAAAGGCACTCCAGAAAGTCTGAAAGATCTAG
  CATCCAAAAACAGACTAGGAAAGGCACTGCCTCTGATGCAGAAAGAATGCACCGACAGAGAAGTCCAACA
  CAATCTCCTCCAGCAGACACATCGTTCAGCAGTCGCAGGGGAAGACAGCTCCCACAAGTGCCAGTGAGAA
  GCGGCAGTATAGAACAAGAGTCGGGCCACAAAAAGTTAAAAAGTACCATCCAGAGAAGCACAGAAACAGG
  CATGGCAGCTGAAATGAGAAAGATGGTAAGGCAGCCGAGCCGAGAGTCTACTGATGGCAGCATCAACAGT
  TACAGCTCTGAGGGCAATTTAATATTTCCTGGAGTGCGACTGGGAGCTGACAGTCAATTCAGTGATTTTC
  TTGATGGATTGGGACCAGCCCAGCTTGTTGGCCGCCAAACCCTTGCCACCCCTGCAATGGGTGATATACA
  AATAGGAATGGAGGACAAAAAGGGCCAATTAGAAGTGGAAGTCATTAGAGCACGAAGCCTCACACAAAAG
  CCTGGTTCCAAATCTACACCTGCTCCATATGTCAAAGTATATCTTTTGGAAAATGGGGCCTGTATAGCCA
  AGAAGAAGACAAGAATTGCACGAAAAACCCTTGATCCTTTGTATCAGCAGTCTCTGGTTTTTGATGAAAG
  TCCACAGGGTAAAGTTCTTCAGGTGATTGTCTGGGGAGACTATGGCAGAATGGACCACAAATGCTTTATG
  GGTGTGGCTCAGATCTTGTTGGAAGAACTCGACCTGTCCAGCATGGTGATCGGATGGTACAAATTGTTCC
  CACCGTCCTCACTGGTGGATCCCACACTCACTCCCCTCACCCGGCGGGCTTCCCAGTCATCTCTGGAAAG
  TTCAACTGGGCCTCCCTGTATTCGATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230521 representing NM_001168410
  Red=Cloning site Green=Tags(s)
  
  MFAGFLQFLLLHTLHSGTGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRLIGRVILNKRTTMPKD
  SGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKS
  EPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLW
  YDKVGHQLIVNVLQATDLPARVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKILEPKWNQTFVYSHVHRR
  DFRERMLEITVWDQPRVQEEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMPRRHIHG
  ESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRSSARESKSTTLTVPEQQRTTHHRSRSVSPHRGND
  QGKPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPVDHRTRDVDSQYLSEQDSELLMLPRAKRGRS
  AECLHTTSELQPFLDRARSASTNCLRPDTSLHSPERERHSRKSERSSIQKQTRKGTASDAERMHRQRSPT
  QSPPADTSFSSRRGRQLPQVPVRSGSIEQESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINS
  YSSEGNLIFPGVRLGADSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQK
  PGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFM
  GVAQILLEELDLSSMVIGWYKLFPPSSLVDPTLTPLTRRASQSSLESSTGPPCIRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168410
• ORF Size: 2478 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001168410.1, NP_001161882.1
• RefSeq ORF: 2481
• Locus ID: 22999
• MW: 93.2 kDa
• Gene Summary: The protein encoded by this gene is a RAS gene superfamily member that regulates synaptic vesicle exocytosis. This gene also plays a role in the regulation of voltage-gated calcium channels during neurotransmitter and insulin release. Mutations have suggested a role cognition and have been identified as the cause of cone-rod dystrophy type 7. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012]