RPH3AL (NM_001190413) Human Tagged ORF Clone

CAT#: RC230793

RPH3AL (Myc-DDK-tagged)-Human rabphilin 3A-like (without C2 domains) (RPH3AL), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY433792) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230793 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPH3AL
• Synonyms: NOC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230793 representing NM_001190413
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACACCATCTTCGGCAGCGGGAATGATCAGTGGGTTTGCCCCAATGACCGGCAGCTTGCCCTTC
  GAGCCAAGCTGCAGACGGGCTGGTCCGTGCACACCTACCAGACGGAGAAGCAGAGGAGGAAGCAGCACCT
  CAGCCCGGCGGAGGTGGAGGCCATCCTGCAGGTCATCCAGAGGGCAGAGCGGCTCGACGTCCTGGAGCAG
  CAGAGAATCGGGCGGCTGGTGGAGCGGCTGGAGACCATGAGGCGGAATGTGATGGGGAACGGCCTGTCCC
  AGTGTCTGCTCTGCGGGGAGGTGCTGGGCTTCCTGGGCAGCTCGTCGGTGTTCTGCAAAGACTGCAGGAA
  GGTCTGGAAGAGGTCGGGGGCCTGGTTCTACAAAGGGCTCCCCAAGTATATCTTGCCCCTGAAGACCCCT
  GGCCGAGCTGATGACCCCCACTTCCGACCTTTGCCCACGGAACCGGCAGAGCGAGAGCCCAGAAGCTCTG
  AGACCAGCCGCATCTACACGTGGGCCCGAGGAAGAGTGGTTTCCAGTGACAGTGACAGTGACTCGGATCT
  TAGCTCCTCCAGCCTAGAGGACAGACTCCCATCCACTGGGGTCAGGGACCGGAAAGGCGACAAACCCTGG
  AAGGAGTCAGGTGGCAGCGTGGAGGCCCCCAGGATGGGGTTCACCCACCCGCCGGGCCACCTCTCTGGGT
  GCCAGAGCAGCCTGGCCAGTGGTGAGACGGGGACAGGCTCTGCTGACCCGCCAGGGGGACCCCGCCCCGG
  GCTGACCCGAAGGGCCCCGGTAAAAGACACACCTGGACGAGCCCCCGCTGCTGACGCAGCTCCAGCAGGC
  CCCTCCAGCTGCCTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230793 representing NM_001190413
  Red=Cloning site Green=Tags(s)
  
  MADTIFGSGNDQWVCPNDRQLALRAKLQTGWSVHTYQTEKQRRKQHLSPAEVEAILQVIQRAERLDVLEQ
  QRIGRLVERLETMRRNVMGNGLSQCLLCGEVLGFLGSSSVFCKDCRKVWKRSGAWFYKGLPKYILPLKTP
  GRADDPHFRPLPTEPAEREPRSSETSRIYTWARGRVVSSDSDSDSDLSSSSLEDRLPSTGVRDRKGDKPW
  KESGGSVEAPRMGFTHPPGHLSGCQSSLASGETGTGSADPPGGPRPGLTRRAPVKDTPGRAPAADAAPAG
  PSSCLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190413
• ORF Size: 858 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001190413.1, NP_001177342.1
• RefSeq ORF: 861
• Locus ID: 9501
• Protein Families: Secreted Protein
• MW: 31.6 kDa
• Gene Summary: The protein encoded by this gene plays a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells and plays a key role in insulin secretion by pancreatic cells. This gene is likely a tumor suppressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jun 2010]