DCXR (NM_001195218) Human Tagged ORF Clone

CAT#: RC231089

DCXR (Myc-DDK-tagged)-Human dicarbonyl/L-xylulose reductase (DCXR), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY434088) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231089 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCXR
• Synonyms: DCR; HCR2; HCRII; KIDCR; P34H; PNTSU; SDR20C1; XR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231089 representing NM_001195218
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGTTCCTCGCGGGCCGCCGGGTGCTGGTCACCGGGGCAGGCAAAGGGCGCGGCACGGTCCAGG
  CGCTGCACGCGACGGGCGCGCGGGTGGTGGCTGTGAGCCGGACTCAGGCGGATCTTGACAGCCTTGTCCG
  CGAGTGCCCGGGGATAGAACCCGTGTGCGTGGACCTGGGTGACTGGGAGGCCACCGAGCGGGCGCTGGGC
  AGCGTGGGCCCCGTGGACCTGCTGGTGAACAACGCCGCTGTCGCCCTGCTGCAGCCCTTCCTGGAGGTCA
  CCAAGGAGGCCTTTGACAGATCCTTTGAGGTGAACCTGCGTGCGGTCATCCAGGTGTCGCAGATTGTGGC
  CAGGGGCTTAATAGCCCGGGGAGTCCCAGGGGCCATCGTGAATGTCTCCAGCCAGTGCTCCCAGCGGGCA
  GTAACTAACCATAGCGTCTACTGCTCCACCAAGGGTGCCCTGGACATGCTGACCAAGGTGATGGCCCTAG
  AGCTCGGGCCCCACAAGATCCGAGTGAATGCAGTAAACCCCACAGTGGTGATGACGTCCATGGGCCAGGC
  CACCTGGAGTGACCCCCACAAGGCCAAGACTATGCTGAACCGAATCCCACTTGGCAAGTTTGCTGAGGTA
  GAGCACGTGGTGAACGCCATCCTCTTTCTGCTGAGTGACCGAAGTGGCATGACCACGGGTTCCACTTTGC
  CGGTGGAAGGGGGCTTCTGGGCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231089 representing NM_001195218
  Red=Cloning site Green=Tags(s)
  
  MELFLAGRRVLVTGAGKGRGTVQALHATGARVVAVSRTQADLDSLVRECPGIEPVCVDLGDWEATERALG
  SVGPVDLLVNNAAVALLQPFLEVTKEAFDRSFEVNLRAVIQVSQIVARGLIARGVPGAIVNVSSQCSQRA
  VTNHSVYCSTKGALDMLTKVMALELGPHKIRVNAVNPTVVMTSMGQATWSDPHKAKTMLNRIPLGKFAEV
  EHVVNAILFLLSDRSGMTTGSTLPVEGGFWAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195218
• ORF Size: 726 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001195218.1, NP_001182147.1
• RefSeq ORF: 729
• Locus ID: 51181
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pentose and glucuronate interconversions
• MW: 26.2 kDa
• Gene Summary: The protein encoded by this gene acts as a homotetramer to catalyze diacetyl reductase and L-xylulose reductase reactions. The encoded protein may play a role in the uronate cycle of glucose metabolism and in the cellular osmoregulation in the proximal renal tubules. Defects in this gene are a cause of pentosuria. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010]