C8orf58 (NM_173686) Human Tagged ORF Clone

CAT#: RC231182

C8orf58 (Myc-DDK-tagged)-Human chromosome 8 open reading frame 58 (C8orf58), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY434181) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231182 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C8orf58
• Synonyms: FLJ34715
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231182 representing NM_173686
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGGCCGGCGGCGCGCCTTCGCCGTGGACGGCCGGGATGGGGCTGGCGAGGGCCTGGCACGGGGCT
  GCATAGTGCCTGGAGTCACCAGCACCTACAGACGGATCCCCGACGCTGCCCACGGGTGCTCATCCTGGGA
  GAGAGGTGACAAGTTCAGAGGTGTCGGCAGGGAGGCACTCTTTCTCAAACTGGCCTCCCGGGACTCAGGA
  GTGGAGATGGCAGTTGGGGACAGCCCCCTGGCCGCCTTACCGGGCCTTTCTCAGGACTCCCTGGACTTTG
  AATCCTCAGGGAGTTCTGAGCCCCCCGCCCAGGTAGGCCGACTCCTGGCCAGCCAGAAGCTGGGGGAGGT
  GTTGGAGCGGTCCCGCCGGCTCCCAACAGCTCCCACCAGCTTGTCAGGACAACACCGCTCCCTGCGGCTG
  GCAAGCAAGCCTGAGCGTGAAGTGCCCCTTGGAGCAGGGCAACAGGAGTCCATGGAGGCAGACACAGACC
  TAGAGGCAGGCCTGGAAGAAGAGGCGGTGGGGGGCCTGGGGCCTGGAGCCTGGGCCTGCCTCCCCGGGCA
  GGGTCTTCGCTATCTGGAACACCTGTGCCTGGTGCTGGAGCAGATGGCAAGGCTCCAGCAGCTCTACCTG
  CAGCTGCGGATCCAGAGGCCCCCAGGGGATCCCGGCGAGGAGGAGTCGACCCGAGCCCCTTTACCGTCCC
  CGTTACACACCCCAGGCAATCGGGGGCAGGGGCCATGGGAGCTGCTAAGCCAGACAGAGCACACAGGAGC
  AAAGGCTGCTTCACCCCCAAAGGTGGAGGTGCCCAGTGCCAACCCTCCCAGGCTGCCAGAAACCCCAGTG
  GAGCCAACGTACCACTTGCCATCCTCCCAGGGACACAAGGTCAAGGTCCTGCTCAACCGGATCTGCCGGA
  GAAGCCACCACCACCCTGAGCCCCCTGCCCCTCCTGATGGCTCTGACCCCAGGATCGAGTCCAGGGACCT
  CCCTGAAAGGCCTCAGTGCCGCCCCCACCGGAAGACCTTTATGCCATCATTAGTGGTTAAGAAGCAACGA
  GCAAAAAACCTTTCTGTAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231182 representing NM_173686
  Red=Cloning site Green=Tags(s)
  
  MMGRRRAFAVDGRDGAGEGLARGCIVPGVTSTYRRIPDAAHGCSSWERGDKFRGVGREALFLKLASRDSG
  VEMAVGDSPLAALPGLSQDSLDFESSGSSEPPAQVGRLLASQKLGEVLERSRRLPTAPTSLSGQHRSLRL
  ASKPEREVPLGAGQQESMEADTDLEAGLEEEAVGGLGPGAWACLPGQGLRYLEHLCLVLEQMARLQQLYL
  QLRIQRPPGDPGEEESTRAPLPSPLHTPGNRGQGPWELLSQTEHTGAKAASPPKVEVPSANPPRLPETPV
  EPTYHLPSSQGHKVKVLLNRICRRSHHHPEPPAPPDGSDPRIESRDLPERPQCRPHRKTFMPSLVVKKQR
  AKNLSVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173686
• ORF Size: 1071 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_173686.2, NP_775957.2
• RefSeq ORF: 1074
• Locus ID: 541565
• MW: 39.1 kDa