LIPF (NM_001198829) Human Tagged ORF Clone

CAT#: RC231214

LIPF (Myc-DDK-tagged)-Human lipase, gastric (LIPF), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY434213) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231214 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LIPF
• Synonyms: GL; HGL; HLAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231214 representing NM_001198829
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCCAACGCAAACAGCAGGTCCAAAATGTGGCTGCTTTTAACAATGGCAAGTTTGATATCTGTAC
  TGGGGACTACACATGGTTTGTTTGGAAAATTACATCCTGGAAGCCCTGAAGTGACTATGAACATTAGTCA
  GATGATTACTTATTGGGGATACCCAAATGAAGAATATGAAGTTGTGACTGAAGATGGTTATATTCTTGAA
  GTCAATAGAATTCCTTATGGGAAGAAAAATTCAGGGAATACAGGCCAGAGACCTGTTGTGTTTTTGCAGC
  ATGGTTTGCTTGCATCAGCCACAAACTGGATTTCCAACCTGCCGAACAACAGCCTTGCCTTCATTCTGGC
  AGATGCTGGTTATGATGTGTGGCTGGGCAACAGCAGAGGAAACACCTGGGCCAGAAGAAACTTGTACTAT
  TCACCAGATTCAGTTGAATTCTGGGCTTTCAGCTTTGATGAAATGGCTAAATATGACCTTCCAGCCACAA
  TCGACTTCATTGTAAAGAAAACTGGACAGAAGCAGCTACACTATGTTGGCCATTCCCAGGGCACCACCAT
  TGGTTTTATTGCCTTTTCCACCAATCCCAGCCTGGCTAAAAGAATCAAAACCTTCTATGCTCTAGCTCCT
  GTTGCCACTGTGAAGTATACAAAAAGCCTTATAAACAAACTTAGATTTGTTCCTCAATCCCTCTTCAAGT
  TTATATTTGGTGACAAAATATTCTACCCACACAACTTCTTTGATCAATTTCTTGCTACTGAAGTGTGCTC
  CCGTGAGATGCTGAATCTCCTTTGCAGCAATGCCTTATTTATAATTTGTGGATTTGACAGTAAGAACTTT
  AACACGAGTCGCTTGGATGTGTATCTATCACATAATCCAGCAGGAACTTCTGTTCAAAACATGTTCCATT
  GGACCCAGGCTGTTAAGTCTGGGAAATTCCAAGCTTATGACTGGGGAAGCCCAGTTCAGAATAGGATGCA
  CTATGATCAGTCCCAACCTCCCTACTACAATGTGACAGCCATGAATGTACCAATTGCAGTGTGGAACGGT
  GGCAAGGACCTGTTGGCTGACCCCCAAGATGTTGGCCTTTTGCTTCCAAAACTCCCCAATCTTATTTACC
  ACAAGGAGATTCCTTTTTACAATCACTTGGACTTTATCTGGGCAATGGATGCCCCTCAAGAAGTTTACAA
  TGACATTGTTTCTATGATATCAGAAGATAAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231214 representing NM_001198829
  Red=Cloning site Green=Tags(s)
  
  MFSNANSRSKMWLLLTMASLISVLGTTHGLFGKLHPGSPEVTMNISQMITYWGYPNEEYEVVTEDGYILE
  VNRIPYGKKNSGNTGQRPVVFLQHGLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYY
  SPDSVEFWAFSFDEMAKYDLPATIDFIVKKTGQKQLHYVGHSQGTTIGFIAFSTNPSLAKRIKTFYALAP
  VATVKYTKSLINKLRFVPQSLFKFIFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNF
  NTSRLDVYLSHNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNG
  GKDLLADPQDVGLLLPKLPNLIYHKEIPFYNHLDFIWAMDAPQEVYNDIVSMISEDKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198829
• ORF Size: 1224 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001198829.1, NP_001185758.1
• RefSeq ORF: 1227
• Locus ID: 8513
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways
• MW: 46.8 kDa
• Gene Summary: This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]