ATG16L1 (NM_198890) Human Tagged ORF Clone

CAT#: RC231237

ATG16L1 (Myc-DDK-tagged)-Human ATG16 autophagy related 16-like 1 (S. cerevisiae) (ATG16L1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATG16L1
• Synonyms: APG16L; ATG16A; ATG16L; IBD10; WDR30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231237 representing NM_198890
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCGGGCCTCCGCGCCGCTGACTTCCCCCGCTGGAAGCGCCACATCTCGGAGCAACTGAGGCGCC
  GGGACCGGCTGCAGAGACAGGCGTTCGAGGAGATCATCCTGCAGTATAACAAATTGCTGGAAAAGTCAGA
  TCTTCATTCAGTGTTGGCCCAGAAACTACAGGCTGAAAAGCATGACGTACCAAACAGGCACGAGATAAGG
  AGGCGGCAAGCCCGGCTGCAGAAAGAGCTTGCAGAAGCAGCAAAGGAACCTCTACCAGTCGAACAGGATG
  ATGACATTGAGGTCATTGTGGATGAAACTTCTGATCACACAGAAGAGACCTCTCCTGTGCGAGCCATCAG
  CAGAGCAGCCACGAGACGCTCTGTCTCTTCCTTCCCAGTCCCCCAGGACAATGTGGATACTCATCCTGGT
  TCTGGTAAAGAAGTGAGGGTACCAGCTACTGCCTTGTGTGTCTTCGATGCACATGATGGGGAAGTCAACG
  CTGTGCAGTTCAGTCCAGGTTCCCGGTTACTGGCCACTGGAGGCATGGACCGCAGGGTTAAGCTTTGGGA
  AGTATTTGGAGAAAAATGTGAGTTCAAGGGTTCCCTATCTGGCAGTAATGCAGGAATTACAAGCATTGAA
  TTTGATAGTGCTGGATCTTACCTCTTAGCAGCTTCAAATGATTTTGCAAGCCGAATCTGGACTGTGGATG
  ATTATCGATTACGGCACACACTCACGGGACACAGTGGGAAAGTGCTGTCTGCTAAGTTCCTGCTGGACAA
  TGCGCGGATTGTCTCAGGAAGTCACGACCGGACTCTCAAACTCTGGGATCTACGCAGCAAAGTCTGCATA
  AAGACAGTGTTTGCAGGATCCAGTTGCAATGATATTGTCTGCACAGAGCAATGTGTAATGAGTGGACATT
  TTGACAAGAAAATTCGTTTCTGGGACATTCGATCAGAGAGCATAGTTCGAGAGATGGAGCTGTTGGGAAA
  GATTACTGCCCTGGACTTAAACCCAGAAAGGACTGAGCTCCTGAGCTGCTCCCGTGATGACTTGCTAAAA
  GTTATTGATCTCCGAACAAATGCTATCAAGCAGACATTCAGTGCACCTGGGTTCAAGTGCGGCTCTGACT
  GGACCAGAGTTGTCTTCAGCCCTGATGGCAGTTACGTGGCGGCAGGCTCTGCTGAGGGCTCTCTGTATAT
  CTGGAGTGTGCTCACAGGGAAAGTGGAAAAGGTTCTTTCAAAGCAGCACAGCTCATCCATCAATGCGGTG
  GCGTGGTCGCCCTCTGGCTCGCACGTTGTCAGTGTGGACAAAGGATGCAAAGCTGTGCTGTGGGCACAGT
  AC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231237 representing NM_198890
  Red=Cloning site Green=Tags(s)
  
  MSSGLRAADFPRWKRHISEQLRRRDRLQRQAFEEIILQYNKLLEKSDLHSVLAQKLQAEKHDVPNRHEIR
  RRQARLQKELAEAAKEPLPVEQDDDIEVIVDETSDHTEETSPVRAISRAATRRSVSSFPVPQDNVDTHPG
  SGKEVRVPATALCVFDAHDGEVNAVQFSPGSRLLATGGMDRRVKLWEVFGEKCEFKGSLSGSNAGITSIE
  FDSAGSYLLAASNDFASRIWTVDDYRLRHTLTGHSGKVLSAKFLLDNARIVSGSHDRTLKLWDLRSKVCI
  KTVFAGSSCNDIVCTEQCVMSGHFDKKIRFWDIRSESIVREMELLGKITALDLNPERTELLSCSRDDLLK
  VIDLRTNAIKQTFSAPGFKCGSDWTRVVFSPDGSYVAAGSAEGSLYIWSVLTGKVEKVLSKQHSSSINAV
  AWSPSGSHVVSVDKGCKAVLWAQY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198890
• ORF Size: 1332 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_198890.2, NP_942593.2
• RefSeq ORF: 1335
• Locus ID: 55054
• MW: 49.9 kDa
• Gene Summary: The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]