A1CF (NM_001198819) Human Tagged ORF Clone

CAT#: RC231331

A1CF (Myc-DDK-tagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: A1CF
• Synonyms: ACF; ACF64; ACF65; APOBEC1CF; ASP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231331 representing NM_001198819
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCAGTGTGTCTGGGCACATGCCCAGAGCCAGAAGCGAGCATGAGCACAGCAATTCCTGGCCTCA
  AAAAAGGAAATAATGCTTTGCAGTCCATCATTTTGCAAACTTTGCTGGAAAAGGAAAATGGACAAAGAAA
  ATATGGTGGCCCTCCACCTGGTTGGGATGCTGCACCCCCTGAAAGGGGCTGTGAAATTTTTATTGGAAAA
  CTTCCCCGAGACCTTTTTGAGGATGAGCTTATACCATTATGTGAAAAAATCGGTAAAATTTATGAAATGA
  GAATGATGATGGATTTTAATGGCAACAATAGAGGATATGCATTTGTAACATTTTCAAATAAAGTGGAAGC
  CAAGAATGCAATCAAGCAACTTAATAATTATGAAATTAGAAATGGGCGCCTCTTAGGGGTTTGTGCCAGT
  GTGGACAACTGCCGATTATTTGTTGGGGGCATCCCAAAAACCAAAAAGAGAGAAGAAATCTTATCGGAGA
  TGAAAAAGGTTACTGAAGGTGTTGTCGATGTCATCGTCTACCCAAGCGCTGCAGATAAAACCAAAAACCG
  AGGCTTTGCCTTCGTGGAGTATGAGAGTCATCGAGCAGCTGCCATGGCGAGGAGGAAACTGCTACCAGGA
  AGAATTCAGTTATGGGGACATGGTATTGCAGTAGACTGGGCAGAGCCAGAAGTAGAAGTTGATGAAGATA
  CAATGTCTTCAGTGAAAATCCTATATGTAAGAAATCTTATGCTGTCTACCTCTGAAGAGATGATTGAAAA
  GGAATTCAACAATATCAAACCAGGTGCTGTGGAGAGGGTGAAGAAAATTCGAGACTATGCTTTTGTGCAC
  TTCAGTAACCGAGAAGATGCAGTTGAGGCTATGAAAGCTTTAAATGGCAAGGTGCTGGATGGTTCCCCCA
  TTGAAGTCACCCTAGCAAAACCAGTGGACAAGGACAGTTATGTTAGGTATACCCGAGGCACAGGTGGAAG
  GGGCACCATGCTGCAAGGAGAGTATACCTACTCTTTGGGCCAAGTTTATGATCCCACCACAACCTACCTT
  GGAGCTCCTGTCTTCTATGCCCCCCAGACCTATGCAGCAATTCCCAGTCTTCATTTCCCAGCCACCAAAG
  GACATCTCAGCAACAGAGCCATTATCCGAGCCCCTTCTGTTAGAGAAATTTACATGAATGTACCTGTAGG
  GGCTGCGGGAGTGAGAGGACTGGGCGGCCGTGGCTATTTGGCATACACAGGCCTGGGTCGAGGATACCAG
  GTCAAAGGAGACAAAAGAGAAGACAAACTCTATGACATTTTACCTGGGATGGAGCTCACCCCAATGAATC
  CTGTCACATTAAAACCCCAAGGAATTAAACTCGCTCCCCAGATATTAGAAGAGATTTGTCAGAAAAATAA
  CTGGGGACAGCCAGTGTACCAGCTGCACTCTGCTATTGGACAAGACCAAAGACAGCTATTCTTGTACAAA
  ATAACTATTCCTGCTCTAGCCAGCCAGAATCCTGCAATCCACCCTTTCACACCTCCAAAGCTGAGTGCCT
  TTGTGGATGAAGCAAAGACGTATGCAGCCGAATACACCCTGCAGACCCTGGGCATCCCCACTGATGGAGG
  CGATGGCACCATGGCTACTGCTGCTGCTGCTGCTACTGCTTTCCCAGGATATGCTGTCCCTAATGCAACT
  GCACCCGTGTCTGCAGCCCAGCTCAAGCAAGCGGTAACCCTTGGACAAGACTTAGCAGCATATACAACCT
  ATGAGGTCTACCCAACTTTTGCAGTGACTGCCCGAGGGGATGGATATGGCACCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231331 representing NM_001198819
  Red=Cloning site Green=Tags(s)
  
  MEAVCLGTCPEPEASMSTAIPGLKKGNNALQSIILQTLLEKENGQRKYGGPPPGWDAAPPERGCEIFIGK
  LPRDLFEDELIPLCEKIGKIYEMRMMMDFNGNNRGYAFVTFSNKVEAKNAIKQLNNYEIRNGRLLGVCAS
  VDNCRLFVGGIPKTKKREEILSEMKKVTEGVVDVIVYPSAADKTKNRGFAFVEYESHRAAAMARRKLLPG
  RIQLWGHGIAVDWAEPEVEVDEDTMSSVKILYVRNLMLSTSEEMIEKEFNNIKPGAVERVKKIRDYAFVH
  FSNREDAVEAMKALNGKVLDGSPIEVTLAKPVDKDSYVRYTRGTGGRGTMLQGEYTYSLGQVYDPTTTYL
  GAPVFYAPQTYAAIPSLHFPATKGHLSNRAIIRAPSVREIYMNVPVGAAGVRGLGGRGYLAYTGLGRGYQ
  VKGDKREDKLYDILPGMELTPMNPVTLKPQGIKLAPQILEEICQKNNWGQPVYQLHSAIGQDQRQLFLYK
  ITIPALASQNPAIHPFTPPKLSAFVDEAKTYAAEYTLQTLGIPTDGGDGTMATAAAAATAFPGYAVPNAT
  APVSAAQLKQAVTLGQDLAAYTTYEVYPTFAVTARGDGYGTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198819
• ORF Size: 1806 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001198819.1, NP_001185748.1
• RefSeq ORF: 1809
• Locus ID: 29974
• MW: 66.4 kDa
• Gene Summary: Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]