GGCT (NM_001199815) Human Tagged ORF Clone

CAT#: RC231931

GGCT (Myc-DDK tagged) - Homo sapiens gamma-glutamylcyclotransferase (GGCT), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GGCT
• Synonyms: C7orf24; CRF21; GCTG; GGC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231931 representing NM_001199815
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACTCGGGCTGCAAGGACGTCACGGGTCCAGATGAGGAGAGTTTTCTGTACTTTGCCTACGGCA
  GCAACCTGCTGACAGAGAGGATCCACCTCCGAAACCCCTCGGCGGCGTTCTTCTGTGTGGCCCGCCTGCA
  GGATTTTAAGCTTGACTTTGGCAATTCCCAAGGCAAAACAAGTCAAACTTGGCATGGAGGGATAGCCACC
  ATTTTTCAGAGTCCTGGCGATGAAGTGTGGGGAGTAGTATGGAAAATGAACAAAAGCAATTTAAATTCTC
  TGGATGAGTGGAGAAAGAAACGTCACACTGCCTTTCAGAATCAGGGAAATCACCCTTGCAAGCACAAGAC
  AAGAATGTGGGATAGAGACCCCAAAATTCCTGTTCAGAACCTAAGCTTAGCTCTCTGTTGGCAGGCCCAA
  TCAGGTCATGGCACCTGTAATAGATTATTTGCATGGGTGCAAAAGAAAATGGTTTGCCGCTGGAGTATCA
  AGAGAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231931 representing NM_001199815
  Red=Cloning site Green=Tags(s)
  
  MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKTSQTWHGGIAT
  IFQSPGDEVWGVVWKMNKSNLNSLDEWRKKRHTAFQNQGNHPCKHKTRMWDRDPKIPVQNLSLALCWQAQ
  SGHGTCNRLFAWVQKKMVCRWSIKRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199815
• ORF Size: 498 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001199815.1, NP_001186744.1
• RefSeq Size: 1217
• RefSeq ORF: 501
• Locus ID: 79017
• Protein Pathways: Glutathione metabolism
• MW: 19.5 kDa
• Gene Summary: The protein encoded by this gene catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism, and may play a critical role in glutathione homeostasis. The encoded protein may also play a role in cell proliferation, and the expression of this gene is a potential marker for cancer. Pseudogenes of this gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 20. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]