Sprouty 1 (SPRY1) (NM_001258039) Human Tagged ORF Clone
CAT#: RC232471
- TrueORF®
SPRY1 (Myc-DDK tagged) - Homo sapiens sprouty homolog 1, antagonist of FGF signaling (Drosophila) (SPRY1), transcript variant 4
"NM_001258039" in other vectors (2)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SPRY1 |
Synonyms | hSPRY1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC232471 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCCCAAAATCAACATGGCAGTGGCAGTTCGTTAGTTGTGATCCAGCAGCCTTCTTTGGATAGCC GTCAGAGATTAGACTATGAGAGAGAGATTCAGCCTACTGCTATTTTGTCCTTAGACCAGATCAAGGCCAT AAGAGGCAGCAATGAATACACAGAAGGGCCTTCGGTGGTGAAAAGACCTGCTCCTCGGACAGCACCAAGA CAAGAAAAGCATGAAAGGACTCATGAAATCATACCAATTAATGTGAATAATAACTACGAGCACAGACACA CAAGCCACCTGGGACATGCAGTACTCCCAAGTAATGCCAGGGGCCCCATTTTGAGCAGATCAACCAGCAC TGGAAGTGCAGCCAGCTCTGGGAGCAACAGCAGTGCCTCTTCTGAACAGGGACTGTTAGGAAGGTCACCA CCAACCAGACCAGTCCCTGGTCATAGGTCTGAAAGGGCAATCCGGACCCAGCCCAAGCAACTGATTGTGG ATGACTTGAAGGGTTCCTTGAAAGAGGACCTGACACAGCACAAGTTCATTTGTGAACAGTGTGGGAAGTG CAAGTGTGGAGAATGCACTGCTCCCAGGACCCTACCATCCTGTTTGGCCTGTAACCGGCAGTGCCTTTGC TCTGCTGAGAGCATGGTGGAATATGGAACCTGCATGTGCTTAGTCAAGGGCATCTTCTACCACTGCTCCA ATGACGACGAAGGGGATTCCTATTCAGATAATCCTTGCTCCTGTTCACAATCACACTGCTGCTCTAGATA CCTGTGTATGGGAGCCATGTCTTTATTTTTACCTTGCTTACTCTGTTATCCTCCTGCTAAAGGATGCCTG AAGCTGTGCAGGAGGTGTTATGACTGGATCCATCGCCCAGGGTGCAGATGTAAGAACTCCAACACTGTCT ATTGTAAGCTGGAGAGCTGCCCCTCCCGGGGTCAGGGTAAACCATCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC232471 protein sequence
Red=Cloning site Green=Tags(s) MDPQNQHGSGSSLVVIQQPSLDSRQRLDYEREIQPTAILSLDQIKAIRGSNEYTEGPSVVKRPAPRTAPR QEKHERTHEIIPINVNNNYEHRHTSHLGHAVLPSNARGPILSRSTSTGSAASSGSNSSASSEQGLLGRSP PTRPVPGHRSERAIRTQPKQLIVDDLKGSLKEDLTQHKFICEQCGKCKCGECTAPRTLPSCLACNRQCLC SAESMVEYGTCMCLVKGIFYHCSNDDEGDSYSDNPCSCSQSHCCSRYLCMGAMSLFLPCLLCYPPAKGCL KLCRRCYDWIHRPGCRCKNSNTVYCKLESCPSRGQGKPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001258039 |
ORF Size | 957 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001258039.1, NP_001244968.1 |
RefSeq Size | 2370 bp |
RefSeq ORF | 960 bp |
Locus ID | 10252 |
Protein Families | Druggable Genome |
Protein Pathways | Jak-STAT signaling pathway |
MW | 35.1 kDa |
Gene Summary | May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. [UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC330632 | SPRY1 (untagged) - Homo sapiens sprouty homolog 1, antagonist of FGF signaling (Drosophila) (SPRY1), transcript variant 4 |
USD 330.00 |
|
RG232471 | SPRY1 (GFP-tagged) - Homo sapiens sprouty homolog 1, antagonist of FGF signaling (Drosophila) (SPRY1), transcript variant 4 |
USD 460.00 |
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