YY1AP1 (NM_001198901) Human Tagged ORF Clone

CAT#: RC233121

YY1AP1 (Myc-DDK tagged) - Homo sapiens YY1 associated protein 1 (YY1AP1), transcript variant 8

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: YY1AP1
• Synonyms: GRNG; HCCA1; HCCA2; YY1AP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233121 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATCTGTTTGAAACTTTCCAAGATGAGATGGGATTCTCCAACATGGAAGATGATGGCCCAGAAG
  AGGAGGAGCGTGTGGCTGAGCCTCAAGCTAACTTTAACACCCCTCAAGCTCTACGGTTTGAGGAACTACT
  GGCCAACCTACTAAATGAACAACATCAGATAGCGAAGGAACTATTTGAACAGCTGAAGATGAAGAAACCT
  TCAGCCAAACAGCAGAAGGAGGTAGAGAAGGTTAAACCCCAGTGTAAGGAAGTTCATCAGACCCTGATTC
  TGGACCCAGCACAAAGGAAGAGACTCCAGCAGCAGATGCAGCAGCATGTTCAGCTCTTGACACAAATCCA
  CCTTCTTGCCACCTGCAACCCCAATCTCAATCCGGAGGCCAGTAGCACCAGGATATGTCTTAAAGAGCTG
  GGAACCTTTGCTCAAAGCTCCATCGCCCTTCACCATCAGTACAACCCCAAGTTTCAGACCCTGTTCCAAC
  CCTGTAACTTGATGGGAGCTATGCAGCTGATTGAAGACTTCAGCACACATGTCAGCATTGACTGCAGCCC
  TCATAAAACTGTCAAGAAGACTGCCAATGAATTTCCCTGTTTGCCAAAGCAAGTGGCTTGGATCCTGGCC
  ACAAGCAAGGTTTTCATGTATCCAGAGTTACTTCCAGTGTGTTCCCTGAAGGCAAAGAATCCCCAGGATA
  AGATCCTCTTCACCAAGGCTGAGGACAATTTGTTAGCTTTAGGACTGAAGCATTTTGAAGGGACTGAGTT
  TCTTAACCCTCTAATCAGCAAGTACCTTCTAACCTGCAAGACTGCCCGCCAACTGACAGTGAGAATCAAG
  AACCTCAACATGAACAGAGCTCCTGACAACATCATTAAATTTTATAAGAAGACCAAACAGCTGCCAGTCC
  TAGGAAAATGCTGTGAAGAGATCCAGCCACATCAGTGGAAGCCACCTATAGAGAGAGAAGAACACCGGCT
  CCCATTCTGGTTAAAGGCCAGTCTGCCATCCATCCAGGAAGAACTGCGGCACATGGCTGATGGTGCTAGA
  GAGGTAGGAAATATGACTGGAACCACTGAGATCAACTCAGATCAAGGCCTAGAAAAAGACAACTCAGAGT
  TGGGGAGTGAAACTCGGTACCCACTGCTATTGCCTAAGGGTGTAGTCCTGAAACTGAAGCCAGTTGCCGA
  CCGTTTCCCCAAGAAGGCTTGGAGACAGAAGCGTTCATCAGTCCTGAAACCCCTCCTTATCCAACCCAGC
  CCCTCTCTCCAGCCCAGCTTCAACCCTGGGAAAACACCAGCCCAATCAACTCATTCAGAAGCCCCTCCGA
  GCAAAATGGTGCTCCGGATTCCTCACCCAATACAGCCAGCCACTGTTTTACAGACAGTTCCAGGTGTCCC
  TCCACTGGGGGTCAGTGGAGGTGAGAGTTTTGAGTCTCCTGCAGCACTGCCTGCTATGCCCCCTGAGGCC
  AGGACAAGCTTCCCTCTGTCTGAGTCCCAGACTTTGCTCTCTTCTGCCCCTGTGCCCAAGGTAATGATGC
  CCTCCCCTGCCTCTTCCATGTTTCGAAAGCCATATGTGAGACGGAGACCCTCAAAAAGAAGGGGAGCCAG
  GGCCTTTCGCTGTATCAAACCTGCCCCTGTTATCCACCCTGCATCTGTTATCTTCACTGTTCCTGCTACC
  ACTGTGAAGATTGTGAGCCTTGGCGGTGGCTGTAACATGATCCAGCCTGTCAATGCGGCTGTGGCCCAGA
  GTCCCCAGACTATTCCCATCGCCACCCTCTTGGTTAACCCTACTTCCTTCCCCTGTCCATTGAACCAGCC
  CCTTGTGGCCTCCTCTGTCTCACCCTTAATTGTTTCTGGCAATTCTGTGAATCTTCCTATACCATCCACC
  CCTGAAGATAAGGCCCACATGAATGTGGACATTGCTTGTGCTGTGGCTGATGGGGAAAATGCCTTTCAGG
  GCCTAGAACCCAAATTAGAGCCCCAGGAACTATCTCCTCTCTCTGCTACTGTTTTCCCCAAAGTGGAACA
  TAGCCCAGGGCCTCCACCAGTCGATAAACAGTGCCAAGAAGGATTGTCAGAGAACAGTGCCTATCGCTGG
  ACCGTTGTGAAAACAGAGGAGGGAAGGCAAGCTCTGGAGCCGCTCCCTCAGGGCATCCAGGAGTCTCTAA
  ACAACTCTTCCCCTGGGGATTTAGAGGAAGTTGTCAAGATGGAACCTGAAGATGCTACAGAGGAAATCAG
  TGGATTTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233121 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDLFETFQDEMGFSNMEDDGPEEEERVAEPQANFNTPQALRFEELLANLLNEQHQIAKELFEQLKMKKP
  SAKQQKEVEKVKPQCKEVHQTLILDPAQRKRLQQQMQQHVQLLTQIHLLATCNPNLNPEASSTRICLKEL
  GTFAQSSIALHHQYNPKFQTLFQPCNLMGAMQLIEDFSTHVSIDCSPHKTVKKTANEFPCLPKQVAWILA
  TSKVFMYPELLPVCSLKAKNPQDKILFTKAEDNLLALGLKHFEGTEFLNPLISKYLLTCKTARQLTVRIK
  NLNMNRAPDNIIKFYKKTKQLPVLGKCCEEIQPHQWKPPIEREEHRLPFWLKASLPSIQEELRHMADGAR
  EVGNMTGTTEINSDQGLEKDNSELGSETRYPLLLPKGVVLKLKPVADRFPKKAWRQKRSSVLKPLLIQPS
  PSLQPSFNPGKTPAQSTHSEAPPSKMVLRIPHPIQPATVLQTVPGVPPLGVSGGESFESPAALPAMPPEA
  RTSFPLSESQTLLSSAPVPKVMMPSPASSMFRKPYVRRRPSKRRGARAFRCIKPAPVIHPASVIFTVPAT
  TVKIVSLGGGCNMIQPVNAAVAQSPQTIPIATLLVNPTSFPCPLNQPLVASSVSPLIVSGNSVNLPIPST
  PEDKAHMNVDIACAVADGENAFQGLEPKLEPQELSPLSATVFPKVEHSPGPPPVDKQCQEGLSENSAYRW
  TVVKTEEGRQALEPLPQGIQESLNNSSPGDLEEVVKMEPEDATEEISGFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198901
• ORF Size: 2250 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198901.1, NP_001185830.1
• RefSeq Size: 2604 bp
• RefSeq ORF: 2253 bp
• Locus ID: 55249
• MW: 83.1 kDa
• Gene Summary: The encoded gene product presumably interacts with YY1 protein; however, its exact function is not known. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]