BLNK (NM_001258442) Human Tagged ORF Clone

CAT#: RC233692

BLNK (Myc-DDK tagged) - Homo sapiens B-cell linker (BLNK), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BLNK
• Synonyms: AGM4; BASH; bca; BLNK-S; LY57; SLP-65; SLP65
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233692 representing NM_001258442
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAGCTTAATAAAATAACCGTCCCCGCCAGTCAGAAGTTGAGGCAGCTTCAAAAGATGGTCCATG
  ATATTAAAAACAATGAAGGTGGAATAATGAATAAAATCAAAAAGCTAAAAGTCAAAGCACCTCCAAGTGT
  TCCTCGAAGGGACTACGCTTCAGAGAGCCCTGCTGACGAAGAGGAGCAGTGGTCCGATGACTTTGACAGC
  GACTATGAAAATCCAGATGAGCACTCGGACTCAGAGATGTACGTGATGCCCGCCGAGGAGAACGCTGATG
  ACAGCTACGAGCCGCCTCCAGTAGAGCAGGAAACCAGGCCGGTTCACCCAGCCCTGCCCTTCGCCAGAGG
  AACAGCTTCAGGTCGAAACAGTGGGGCCTGGGAAACCAAGTCACCTCCACCAGCTGCACCATCCCCGTTG
  CCACGGGCCGGGAAAAAACCAACGACACCACTGAAGACAACTCCAGTTGCCTCTCAACAGAATGCTTCAA
  GTGTTTGTGAAGAAAAACCTATACCTGCTGAACGCCACCGAGGGTCAAGTCACAGACAAGAAGCTGTGCA
  GTCACCAGTGTTTCCTCCTGCCCAGAAACAAATCCACCAAAAACCCATACCTCTGCCAAGATTTACAGAA
  GGGGGAAACCCAACTGTGGATGGGCCCCTACCCAGCTTTTCATCTAATTCCACTATTTCAGAACAGGAAG
  CTGGCGTTCTCTGCAAGCCATGGTATGCTGGAGCCTGTGATCGAAAGTCTGCTGAAGAGGCATTGCACAG
  ATCAAACAAGTACTTTGGAAGTGTTGCTGAAATCATCAGGAATCATCAACATAGTCCTTTGGTTCTTATT
  GACAGTCAGAATAACACAAAAGATTCCACCAGACTGAAGTATGCAGTTAAAGTTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233692 representing NM_001258442
  Red=Cloning site Green=Tags(s)
  
  MDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMNKIKKLKVKAPPSVPRRDYASESPADEEEQWSDDFDS
  DYENPDEHSDSEMYVMPAEENADDSYEPPPVEQETRPVHPALPFARGTASGRNSGAWETKSPPPAAPSPL
  PRAGKKPTTPLKTTPVASQQNASSVCEEKPIPAERHRGSSHRQEAVQSPVFPPAQKQIHQKPIPLPRFTE
  GGNPTVDGPLPSFSSNSTISEQEAGVLCKPWYAGACDRKSAEEALHRSNKYFGSVAEIIRNHQHSPLVLI
  DSQNNTKDSTRLKYAVKVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258442
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001258442.1, NP_001245371.1
• RefSeq Size: 1298
• RefSeq ORF: 900
• Locus ID: 29760
• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway, Primary immunodeficiency
• MW: 33.4 kDa
• Gene Summary: This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]