SLC39A1 (NM_001271958) Human Tagged ORF Clone

CAT#: RC233762

SLC39A1 (Myc-DDK tagged) - Homo sapiens solute carrier family 39 (zinc transporter), member 1 (SLC39A1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC39A1
• Synonyms: ZIP1; ZIRTL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233762 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCTGGGGAGAGCCAGAGCTCCTGGTGTGGCGCCCCGAGGCGGTAGCTTCAGAGCCTCCAGTGC
  CTGTGGGGCTGGAGGTGAAGTTGGGGGCCCTGGTGCTGCTGCTGGTGCTCACCCTCCTCTGCAGCCTGGT
  GCCCATCTGTGTGCTGCGCCGGCCAGGAGCTAACCATGAAGGCTCAGCTTCCCGCCAGAAAGCCCTGAGC
  CTAGTAAGCTGTTTCGCGGGGGGCGTCTTTTTGGCCACTTGTCTCCTGGACCTGCTGCCTGACTACCTGG
  CTGCCATAGATGAGGCCCTGGCAGCCTTGCACGTGACGCTCCAGTTCCCACTGCAAGAGTTCATCCTGGC
  CATGGGCTTCTTCCTGGTCCTGGTGATGGAGCAGATCACACTGGCTTACAAGGAGCAGTCAGGGCCGTCA
  CCTCTGGAGGAAACAAGGGCTCTGCTGGGAACAGTGAATGGTGGGCCGCAGCATTGGCATGATGGGCCAG
  GGGTCCCACAGGCGAGTGGAGCCCCAGCAACCCCCTCAGCCTTGCGTGCCTGTGTACTGGTGTTCTCCCT
  GGCCCTCCACTCCGTGTTCGAGGGGCTGGCGGTAGGGCTGCAGCGAGACCGGGCTCGGGCCATGGAGCTG
  TGCCTGGCTTTGCTGCTCCACAAGGGCATCCTGGCTGTCAGCCTGTCCCTGCGGCTGTTGCAGAGCCACC
  TTAGGGCACAGGTGGTGGCTGGCTGTGGGATCCTCTTCTCATGCATGACACCTCTAGGCATCGGGCTGGG
  TGCAGCTCTGGCAGAGTCGGCAGGACCTCTGCACCAGCTGGCCCAGTCTGTGCTAGAGGGCATGGCAGCT
  GGCACCTTTCTCTATATCACCTTTCTGGAAATCCTGCCCCAGGAGCTGGCCAGTTCTGAGCAAAGGATCC
  TCAAGGTCATTCTGCTCCTAGCAGGCTTTGCCCTGCTCACTGGCCTGCTCTTCATCCAAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233762 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGPWGEPELLVWRPEAVASEPPVPVGLEVKLGALVLLLVLTLLCSLVPICVLRRPGANHEGSASRQKALS
  LVSCFAGGVFLATCLLDLLPDYLAAIDEALAALHVTLQFPLQEFILAMGFFLVLVMEQITLAYKEQSGPS
  PLEETRALLGTVNGGPQHWHDGPGVPQASGAPATPSALRACVLVFSLALHSVFEGLAVGLQRDRARAMEL
  CLALLLHKGILAVSLSLRLLQSHLRAQVVAGCGILFSCMTPLGIGLGAALAESAGPLHQLAQSVLEGMAA
  GTFLYITFLEILPQELASSEQRILKVILLLAGFALLTGLLFIQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271958
• ORF Size: 972 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271958.1, NP_001258887.1
• RefSeq Size: 2155 bp
• RefSeq ORF: 975 bp
• Locus ID: 27173
• Protein Families: Transmembrane
• MW: 34.2 kDa
• Gene Summary: This gene encodes a member of the zinc-iron permease family. The encoded protein is localized to the cell membrane and acts as a zinc uptake transporter. This gene has been linked to prostate cancer, breast cancer, and Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]