ASCC1 (NM_001198798) Human Tagged ORF Clone

CAT#: RC233863

ASCC1 (Myc-DDK tagged) - Homo sapiens activating signal cointegrator 1 complex subunit 1 (ASCC1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASCC1
• Synonyms: ASC1p50; CGI-18; p50; SMABF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233863 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTTCTGCGTCCACAGCTTATAAGAATTGATGGCCGGAATTACAGGAAGAATCCAGTCCAAGAAC
  AGACCTATCAACATGAAGAAGATGAAGAGGACTTCTATCAAGGCTCCATGGAGTGTGCTGATGAGCCCTG
  TGATGCCTACGAGGTGGAGCAGACCCCACAAGGATTCCGGTCTACTTTGAGGGCCCCCAGCTTGCTCTAT
  AAGCATATAGTTGGAAAGAGAGGGGACACTAGGAAGAAAATAGAAATGGAGACCAAAACTTCTATTAGCA
  TTCCTAAACCTGGACAAGACGGGGAAATTGTAATCACTGGCCAGCATCGAAATGGTGTAATTTCAGCCCG
  AACACGGATTGATGTTCTTTTGGACACTTTTCGAAGAAAGCAGCCCTTCACTCACTTCCTTGCCTTTTTC
  CTCAATGAAGTTGAGGTTCAGGAAGGATTCCTGAGATTCCAGGAGGAAGTACTGGCGAAGTGCTCCATGG
  ATCATGGGGTTGACAGCAGCATTTTCCAGAATCCTAAAAAGCTTCATCTAACTATTGGGATGTTGGTGCT
  TTTGAGTGAGGAAGAGATCCAGCAGACATGTGAGATGCTACAGCAGTGTAAAGAGGAATTCATTAATGAT
  ATTTCTGGGGGTAAACCCCTAGAAGTGGAGATGGCAGGGATAGAATACATGAATGATGATCCTGGCATGG
  TGGATGTTCTTTACGCCAAAGTCCATATGAAAGATGGCTCCAACAGGCTACAAGAATTAGTTGATCGAGT
  GCTGGAACGTTTTCAGGCATCTGGACTAATAGTGAAAGAGTGGAATAGTGTGAAACTGCATGCTACAGTT
  ATGAATACACTATTCAGGAAAGACCCCAATGCTGAAGGCAGGTACAATCTCTACACAGCGGAAGGCAAAT
  ATATCTTCAAGGAAAGAGAATCATTTGATGGCCGAAATATTTTAAAGTTGTTTGAGAACTTCTACTTTGG
  CTCCCTAAAGCTGAATTCAATTCACATCTCTCAGAGGTTCACCGTAGACAGCTTTGGAAACTACGCTTCC
  TGTGGACAAATTGACTTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233863 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEVLRPQLIRIDGRNYRKNPVQEQTYQHEEDEEDFYQGSMECADEPCDAYEVEQTPQGFRSTLRAPSLLY
  KHIVGKRGDTRKKIEMETKTSISIPKPGQDGEIVITGQHRNGVISARTRIDVLLDTFRRKQPFTHFLAFF
  LNEVEVQEGFLRFQEEVLAKCSMDHGVDSSIFQNPKKLHLTIGMLVLLSEEEIQQTCEMLQQCKEEFIND
  ISGGKPLEVEMAGIEYMNDDPGMVDVLYAKVHMKDGSNRLQELVDRVLERFQASGLIVKEWNSVKLHATV
  MNTLFRKDPNAEGRYNLYTAEGKYIFKERESFDGRNILKLFENFYFGSLKLNSIHISQRFTVDSFGNYAS
  CGQIDFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198798
• ORF Size: 1071 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198798.1, NM_001198798.2, NP_001185727.1
• RefSeq Size: 2683 bp
• RefSeq ORF: 1074 bp
• Locus ID: 51008
• MW: 41.2 kDa
• Gene Summary: This gene encodes a subunit of the activating signal cointegrator 1 (ASC-1) complex. The ASC-1 complex is a transcriptional coactivator that plays an important role in gene transactivation by multiple transcription factors including activating protein 1 (AP-1), nuclear factor kappa-B (NF-kB) and serum response factor (SRF). The encoded protein contains an N-terminal KH-type RNA-binding motif which is required for AP-1 transactivation by the ASC-1 complex. Mutations in this gene are associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]