SETMAR (NM_001276325) Human Tagged ORF Clone

CAT#: RC233886

SETMAR (Myc-DDK tagged) - Homo sapiens SET domain and mariner transposase fusion gene (SETMAR), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SETMAR
• Synonyms: Mar1; METNASE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233886 representing NM_001276325
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGCGGAAGCGGCAAAGACGACACGGCCTTGTGGGATGGCGGAGTTTAAGGAGAAGCCTGAGGCCC
  CGACTGAGCAGCTGGATGTCGCGTGCGGCCAGGAAAACTTGCCGGTGGGCGCGTGGCCCCCGGGGGCCGC
  GCCGGCGCCCTTCCAGTACACTCCTGATCATGTAGTTGGACCTGGAGCAGACATTGATCCCACTCAAATA
  ACCTTTCCCGGATGCATTTGTGTCAAAACTCCCTGCCTCCCTGGCACTTGCTCCTGTCTCCGCCATGGAG
  AGAACTATGATGATAACTCATGCCTTAGAGATATAGGATCTGGAGGAAAGTATGCAGAGCCTGTTTTTGA
  ATGCAATGTCCTGTGCCGATGCAGTGACCACTGCAGAAACAGAGTGGTCCAGAAAGGTCTACAGTTCCAC
  TTCCAAGTGTTCAAGACGCATAAAAAAGGCTGGGGACTTCGTACCTTGGAATTTATACCGAAAGGAAGGT
  TTGTCTGTGAATATGCTGGTGAGGTTTTAGGATTCTCTGAAGTTCAGAGAAGAATTCACTTACAAACAAA
  ATCCGACTCCAATTACATTATAGCCATCAGGGAACATGTTTATAATGGGCAGGTAATGGAAACATTTGTT
  GACCCTACTTATATAGGAAATATTGGAAGATTCCTTAATCATTCTTGTGAGCCAAACCTTTTGATGATTC
  CTGTCCGAATTGACTCAATGGTACCTAAGTTGGCACTTTTTGCAGCCAAAGATATTGTGCCAGAAGAAGA
  ACTCTCTTATGATTATTCAGGAAGATATCTTAATCTAACAGTCAGTGAAGACAAAGAAAGGCTAGATCAT
  GGGAAACTAAGGAAACCTTGTTACTGTGGTGCCAAATCATGTACTGCTTTCCTGCCTTTTGACAGTTCTC
  TGTACTGCCCCGTAGAAAAGTCGAACATCAGTTGTGGAAATGAGAAGGAACCCAGCATGTGTGGCTCAGC
  CCCTTCTGTGTTCCCCTCCTGCAAGCGATTGACCCTTGAGGTGAGTCTGTTCAGTGATAAGCAGCTTGCC
  CCTCCCTATAGTGGAAGACAGTGGTTGGCTAGCTTTACCTCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233886 representing NM_001276325
  Red=Cloning site Green=Tags(s)
  
  MFAEAAKTTRPCGMAEFKEKPEAPTEQLDVACGQENLPVGAWPPGAAPAPFQYTPDHVVGPGADIDPTQI
  TFPGCICVKTPCLPGTCSCLRHGENYDDNSCLRDIGSGGKYAEPVFECNVLCRCSDHCRNRVVQKGLQFH
  FQVFKTHKKGWGLRTLEFIPKGRFVCEYAGEVLGFSEVQRRIHLQTKSDSNYIIAIREHVYNGQVMETFV
  DPTYIGNIGRFLNHSCEPNLLMIPVRIDSMVPKLALFAAKDIVPEEELSYDYSGRYLNLTVSEDKERLDH
  GKLRKPCYCGAKSCTAFLPFDSSLYCPVEKSNISCGNEKEPSMCGSAPSVFPSCKRLTLEVSLFSDKQLA
  PPYSGRQWLASFTSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276325
• ORF Size: 1095 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276325.1, NP_001263254.1
• RefSeq Size: 1775 bp
• RefSeq ORF: 1098 bp
• Locus ID: 6419
• Cytogenetics: 3p26.1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Lysine degradation
• MW: 41 kDa
• Gene Summary: 'This gene encodes a fusion protein that contains an N-terminal histone-lysine N-methyltransferase domain and a C-terminal mariner transposase domain. The encoded protein binds DNA and functions in DNA repair activities including non-homologous end joining and double strand break repair. The SET domain portion of this protein specifically methylates histone H3 lysines 4 and 36. This gene exists as a fusion gene only in anthropoid primates, other organisms lack mariner transposase domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]'