PKMYT1 (NM_001258450) Human Tagged ORF Clone

CAT#: RC234078

PKMYT1 (Myc-DDK tagged) - Homo sapiens protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PKMYT1
• Synonyms: MYT1; PPP1R126
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234078 representing NM_001258450
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAGAACGCTGGCACCAGCTGCAGCCCCGGCGGGTGTCATTCCGGGGCGAGGCCTCAGAGACTCTGC
  AGAGCCCTGGGTATGACCCAAGCCGGCCAGAGTCCTTCTTCCAGCAGAGCTTCCAGAGGCTCAGCCGCCT
  GGGCCATGGCTCCTACGGAGAGGTCTTCAAGGTGCGCTCCAAGGAGGACGGCCGGCTCTATGCGGTAAAG
  CGTTCCATGTCACCATTCCGGGGCCCCAAGGACCGGGCCCGCAAGTTGGCCGAGGTGGGCAGCCACGAGA
  AGGTGGGGCAGCACCCATGCTGCGTGCGGCTGGAGCAGGCCTGGGAGGAGGGCGGCATCCTGTACCTGCA
  GACGGAGCTGTGCGGGCCCAGCCTGCAGCAACACTGTGAGGCCTGGGGTGCCAGCCTGCCTGAGGCCCAG
  GTCTGGGGCTACCTGCGGGACACGCTGCTTGCCCTGGCCCATCTGCACAGCCAGGGCCTGGTGCACCTTG
  ATGTCAAGCCTGCCAACATCTTCCTGGGGCCCCGGGGCCGCTGCAAGCTGGGTGACTTCGGACTGCTGGT
  GGAGCTGGGTACAGCAGGAGCTGGTGAGGTCCAGGAGGGAGACCCCCGCTACATGGCCCCCGAGCTGCTG
  CAGGGCTCCTATGGGACAGCAGCGGATGTGTTCAGTCTGGGCCTCACCATCCTGGAAGTGGCATGCAACA
  TGGAGCTGCCCCACGGTGGGGAGGGCTGGCAGCAGCTGCGCCAGGGCTACCTGCCCCCTGAGTTCACTGC
  CGGTCTGTCTTCCGAGCTGCGTTCTGTCCTTGTCATGATGCTGGAGCCAGACCCCAAGCTGCGGGCCACG
  GCCGAGGCCCTGCTGGCACTGCCTGTGTTGAGGCAGCCGCGGGCCTGGGGTGTGCTGTGGTGCATGGCAG
  CGGAGGCCCTGAGCCGAGGGTGGGCCCTGTGGCAGGCCCTGCTTGCCCTGCTCTGCTGGCTCTGGCATGG
  GCTGGCTCACCCTGCCAGCTGGCTACAGCCCCTGGGCCCGCCAGCCACCCCGCCTGGCTCACCACCCTGC
  AGTTTGCTCCTGGACAGCAGCCTCTCCAGCAACTGGGATGACGACAGCCTAGGGCCTTCACTCTCCCCTG
  AGGCTGTCCTGGCCCGGACTGTGGGGAGCACCTCCACCCCCCGGAGCAGGTGCACACCCAGGGATGCCCT
  GGACCTAAGTGACATCAACTCAGAGCCTCCTCGGGGCTCCTTCCCCTCCTTTGAGCCTCGGAACCTCCTC
  AGCCTGTTTGAGGACACCCTAGACCCAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234078 representing NM_001258450
  Red=Cloning site Green=Tags(s)
  
  MLERWHQLQPRRVSFRGEASETLQSPGYDPSRPESFFQQSFQRLSRLGHGSYGEVFKVRSKEDGRLYAVK
  RSMSPFRGPKDRARKLAEVGSHEKVGQHPCCVRLEQAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQ
  VWGYLRDTLLALAHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGTAGAGEVQEGDPRYMAPELL
  QGSYGTAADVFSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLVMMLEPDPKLRAT
  AEALLALPVLRQPRAWGVLWCMAAEALSRGWALWQALLALLCWLWHGLAHPASWLQPLGPPATPPGSPPC
  SLLLDSSLSSNWDDDSLGPSLSPEAVLARTVGSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLL
  SLFEDTLDPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258450
• ORF Size: 1290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001258450.1, NP_001245379.1
• RefSeq Size: 1980
• RefSeq ORF: 1293
• Locus ID: 9088
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
• MW: 47.7 kDa
• Gene Summary: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]