LAT2 (SLC7A8) (NM_001267036) Human Tagged ORF Clone

CAT#: RC234079

SLC7A8 (Myc-DDK tagged) - Homo sapiens solute carrier family 7 (amino acid transporter light chain, L system), member 8 (SLC7A8), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC7A8
• Synonyms: LAT2; LPI-PC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234079 representing NM_001267036
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAGTATGGGCAGGAGCTTAGCTGGAAGTGTTTGGTAAAAGCTGTGTGCCTCCAGGAGCACTCAC
  AGCCCTCACAGCTTCTCTGCACCCTCCTCCTCTGCTGGTGTGTCCTTGGAAGAGAGAGGCCCTTCCGGAA
  GGCTCAGAGCACCTCCTCTCCTTTGGAGGGGGTTCCGAGATTTCTAAAACGTTTGCTCCTCACATGGGTC
  AACTGTTCCAGTGTGCGGTGGGCCACCCGGGTTCAAGACATCTTCACAGCTGGGAAGCTCCTGGCCTTGG
  CCCTGATTATCATCATGGGGATTGTACAGATATGCAAAGGAGAGTACTTCTGGCTGGAGCCAAAGAATGC
  ATTTGAGAATTTCCAGGAACCTGACATCGGCCTCGTCGCACTGGCTTTCCTTCAGGGCTCCTTTGCCTAT
  GGAGGCTGGAACTTTCTGAATTACGTGACTGAGGAGCTTGTTGATCCCTACAAGAACCTTCCCAGAGCCA
  TCTTCATCTCCATCCCACTGGTCACATTTGTGTATGTCTTTGCCAATGTCGCTTATGTCACTGCAATGTC
  CCCCCAGGAGCTGCTGGCATCCAACGCCGTCGCTGTGACTTTTGGAGAGAAGCTCCTAGGAGTCATGGCC
  TGGATCATGCCCATTTCTGTTGCCCTGTCCACATTTGGAGGAGTTAATGGGTCTCTCTTCACCTCCTCTC
  GGCTGTTCTTCGCTGGAGCCCGAGAGGGCCACCTTCCCAGTGTGTTGGCCATGATCCACGTGAAGCGCTG
  CACCCCAATCCCAGCCCTGCTCTTCACATGCATCTCCACCCTGCTGATGCTGGTCACCAGCGACATGTAC
  ACACTCATCAACTATGTGGGCTTCATCAACTACCTCTTCTATGGGGTCACGGTTGCTGGACAGATAGTCC
  TTCGCTGGAAGAAGCCTGATATCCCCCGCCCCATCAAGATCAACCTGCTGTTCCCCATCATCTACTTGCT
  GTTCTGGGCCTTCCTGCTGGTCTTCAGCCTGTGGTCAGAGCCGGTGGTGTGTGGCATTGGCCTGGCCATC
  ATGCTGACAGGAGTGCCTGTCTATTTCCTGGGTGTTTACTGGCAACACAAGCCCAAGTGTTTCAGTGACT
  TCATTGAGCTGCTAACCCTGGTGAGCCAGAAGATGTGTGTGGTCGTGTACCCCGAGGTGGAGCGGGGCTC
  AGGGACAGAGGAGGCTAATGAGGACATGGAGGAGCAGCAGCAGCCCATGTACCAACCCACTCCCACGAAG
  GACAAGGACGTGGCGGGGCAGCCCCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234079 representing NM_001267036
  Red=Cloning site Green=Tags(s)
  
  MGQYGQELSWKCLVKAVCLQEHSQPSQLLCTLLLCWCVLGRERPFRKAQSTSSPLEGVPRFLKRLLLTWV
  NCSSVRWATRVQDIFTAGKLLALALIIIMGIVQICKGEYFWLEPKNAFENFQEPDIGLVALAFLQGSFAY
  GGWNFLNYVTEELVDPYKNLPRAIFISIPLVTFVYVFANVAYVTAMSPQELLASNAVAVTFGEKLLGVMA
  WIMPISVALSTFGGVNGSLFTSSRLFFAGAREGHLPSVLAMIHVKRCTPIPALLFTCISTLLMLVTSDMY
  TLINYVGFINYLFYGVTVAGQIVLRWKKPDIPRPIKINLLFPIIYLLFWAFLLVFSLWSEPVVCGIGLAI
  MLTGVPVYFLGVYWQHKPKCFSDFIELLTLVSQKMCVVVYPEVERGSGTEEANEDMEEQQQPMYQPTPTK
  DKDVAGQPQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001267036
• ORF Size: 1290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001267036.1, NP_001253965.1
• RefSeq Size: 3296
• RefSeq ORF: 1293
• Locus ID: 23428
• Protein Families: Druggable Genome, Transmembrane
• MW: 48.7 kDa
• Gene Summary: Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney. [UniProtKB/Swiss-Prot Function]