GPR176 (NM_001271854) Human Tagged ORF Clone

CAT#: RC234325

GPR176 (Myc-DDK tagged) - Homo sapiens G protein-coupled receptor 176 (GPR176), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR176
• Synonyms: HB-954
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234325 representing NM_001271854.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGACATAACGGGAGCTGGATCTCTCCAAATGCCAGCGAGCCGCACAACGCGTCCGGCGCCGAGGCT
  GCGGGTGTGAACCGCAGCGCGCTCGGGGAGTTCGGCGAGGCGCAGCTGTACCGCCAGTTCACCACCACC
  GTGCAGGTCGTCATCTTCATAGGCTCGCTGCTCGAGTTTGGCAACATGGAGGTCACTAGAAAACTTGAT
  AAGAGCAGACTGCCTGGGATTAAGTTCATTAAAAACCTGGCCTGCTCGGGGATTTGTGCCAGCCTGGTC
  TGTGTGCCCTTCGACATCATCCTCAGCACCAGTCCTCACTGTTGCTGGTGGATCTACACCATGCTCTTC
  TGCAAGGTCGTCAAATTTTTGCACAAAGTATTCTGCTCTGTGACCATCCTCAGCTTCCCTGCTATTGCT
  TTGGACAGGTACTACTCAGTCCTCTATCCACTGGAGAGGAAAATATCTGATGCCAAGTCCCGTGAACTG
  GTGATGTACATCTGGGCCCATGCAGTGGTGGCCAGTGTCCCTGTGTTTGCAGTAACCAATGTGGCTGAC
  ATCTATGCCACGTCCACCTGCACGGAAGTCTGGAGCAACTCCTTGGGCCACCTGGTGTACGTTCTGGTG
  TATAACATCACCACGGTCATTGTGCCTGTGGTGGTGGTGTTCCTCTTCTTGATACTGATCCGACGGGCC
  CTGAGTGCCAGCCAGAAGAAGAAGGTCATCATAGCAGCGCTCCGGACCCCACAGAACACCATCTCTATT
  CCCTATGCCTCCCAGCGGGAGGCCGAGCTGCACGCCACCCTGCTCTCCATGGTGATGGTCTTCATCTTG
  TGTAGCGTGCCCTATGCCACCCTGGTCGTCTACCAGACTGTGCTCAATGTCCCTGACACTTCCGTCTTC
  TTGCTGCTCACTGCTGTTTGGCTGCCCAAAGTCTCCCTGCTGGCAAACCCTGTTCTCTTTCTTACTGTG
  AACAAATCTGTCCGCAAGTGCTTGATAGGGACCCTGGTGCAACTACACCACCGGTACAGTCGCCGTAAT
  GTGGTCAGTACAGGGAGTGGCATGGCTGAGGCCAGCCTGGAACCCAGCATACGCTCGGGTAGCCAGCTC
  CTGGAGATGTTCCACATTGGGCAGCAGCAGATCTTTAAGCCCACAGAGGATGAGGAAGAGAGTGAGGCC
  AAGTACATTGGCTCAGCTGACTTCCAGGCCAAGGAGATATTTAGCACCTGCCTGGAGGGAGAGCAGGGG
  CCACAGTTTGCGCCCTCTGCCCCACCCCTGAGCACAGTGGACTCTGTATCCCAGGTGGCACCGGCAGCC
  CCTGTGGAACCTGAAACATTCCCTGATAAGTATTCCCTGCAGTTTGGCTTTGGGCCTTTTGAGTTGCCT
  CCTCAGTGGCTCTCAGAGACCCGAAACAGCAAGAAGCGGCTGCTTCCCCCCTTGGGCAACACCCCAGAA
  GAGCTGATCCAGACAAAGGTGCCCAAGGTAGGCAGGGTGGAGCGGAAGATGAGCAGAAACAATAAAGTG
  AGCATTTTTCCAAAGGTGGATTCC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC234325
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGHNGSWISPNASEPHNASGAEAAGVNRSALGEFGEAQLYRQFTTTVQVVIFIGSLLEFGNMEVTRKLD
  KSRLPGIKFIKNLACSGICASLVCVPFDIILSTSPHCCWWIYTMLFCKVVKFLHKVFCSVTILSFPAIA
  LDRYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAVTNVADIYATSTCTEVWSNSLGHLVYVLV
  YNITTVIVPVVVVFLFLILIRRALSASQKKKVIIAALRTPQNTISIPYASQREAELHATLLSMVMVFIL
  CSVPYATLVVYQTVLNVPDTSVFLLLTAVWLPKVSLLANPVLFLTVNKSVRKCLIGTLVQLHHRYSRRN
  VVSTGSGMAEASLEPSIRSGSQLLEMFHIGQQQIFKPTEDEEESEAKYIGSADFQAKEIFSTCLEGEQG
  PQFAPSAPPLSTVDSVSQVAPAAPVEPETFPDKYSLQFGFGPFELPPQWLSETRNSKKRLLPPLGNTPE
  ELIQTKVPKVGRVERKMSRNNKVSIFPKVDS
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271854
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271854.2
• RefSeq Size: 4539 bp
• RefSeq ORF: 1545 bp
• Locus ID: 11245
• UniProt ID: Q14439
• Cytogenetics: 15q14-q15.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 56.9 kDa
• Gene Summary: Members of the G protein-coupled receptor family, such as GPR176, are cell surface receptors involved in responses to hormones, growth factors, and neurotransmitters (Hata et al., 1995 [PubMed 7893747]).[supplied by OMIM, Jul 2008]