NUP62 (NM_001193357) Human Tagged ORF Clone

CAT#: RC234347

NUP62 (Myc-DDK tagged) - Homo sapiens nucleoporin 62kDa (NUP62), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NUP62
• Synonyms: IBSN; p62; SNDI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234347 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGAGCGGGTTTAATTTTGGAGGCACTGGGGCCCCTACAGGCGGGTTCACGTTTGGCACTGCAAAGAC
  GGCAACAACCACACCTGCTACAGGGTTTTCTTTCTCCACCTCTGGCACTGGAGGGTTTAATTTTGGGGCT
  CCCTTCCAACCAGCCACAAGTACCCCTTCCACCGGCCTGTTCTCACTTGCCACCCAGACTCCGGCCACAC
  AGACGACAGGCTTCACTTTTGGAACAGCGACTCTTGCTTCGGGGGGAACTGGATTTTCTTTGGGGATCGG
  TGCTTCAAAGCTCAACTTGAGCAACACAGCTGCCACCCCAGCCATGGCAAACCCCAGCGGCTTTGGGCTG
  GGCAGCAGCAACCTCACTAATGCCATATCGAGCACCGTCACCTCCAGCCAGGGCACAGCACCCACCGGCT
  TTGTGTTTGGCCCCTCCACCACCTCTGTGGCTCCAGCTACCACATCTGGAGGCTTCTCATTCACTGGTGG
  AAGCACGGCCCAACCCTCCGGTTTCAACATTGGCTCAGCAGGGAATTCAGCCCAGCCCACGGCACCTGCC
  ACGTTGCCCTTCACTCCGGCCACGCCAGCAGCCACCACAGCAGGTGCCACACAGCCAGCTGCTCCCACAC
  CCACAGCCACCATCACCAGTACTGGGCCCAGCCTCTTTGCGTCAATAGCAACTGCTCCAACCTCATCTGC
  CACCACTGGACTCTCCCTCTGTACCCCTGTGACCACAGCGGGCGCCCCCACTGCTGGGACACAGGGCTTC
  AGCTTAAAGGCACCTGGAGCAGCTTCCGGCACCTCCACAACAACATCCACCGCTGCCACCGCCACCGCCA
  CCACCACCAGCAGCAGCAGCACCACCGGCTTTGCCTTGAATTTAAAACCACTGGCGCCAGCCGGGATCCC
  CAGCAATACAGCAGCTGCCGTGACCGCTCCACCTGGCCCTGGCGCAGCTGCAGGGGCGGCTGCCAGCTCC
  GCCATGACCTACGCGCAGCTGGAGAGCCTGATCAACAAATGGAGCCTGGAGCTAGAGGACCAGGAGCGGC
  ACTTCCTCCAGCAGGCCACCCAGGTCAACGCCTGGGACCGCACGCTGATCGAGAATGGAGAAAAGATCAC
  CAGCCTGCACCGCGAGGTGGAGAAGGTGAAGCTGGACCAGAAGAGGCTGGACCAGGAGCTCGACTTCATC
  CTGTCCCAGCAGAAGGAGCTGGAAGACCTGCTGAGCCCACTGGAGGAGTTGGTCAAGGAGCAGAGCGGGA
  CCATCTACCTGCAGCACGCGGATGAGGAGCGTGAGAAAACCTACAAGCTGGCTGAGAACATCGACGCACA
  GCTCAAGCGCATGGCCCAGGATCTCAAGGACATCATCGAGCACCTGAACACGTCCGGGGCCCCCGCCGAC
  ACCAGTGACCCACTGCAGCAGATCTGCAAGATCCTCAATGCGCACATGGACTCACTGCAGTGGATCGACC
  AGAACTCGGCCCTGCTGCAGAGGAAGGTGGAGGAGGTGACCAAGGTGTGCGAGGGCCGGCGCAAGGAGCA
  GGAGCGCAGCTTCCGGATCACCTTTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234347 protein sequence
  Red=Cloning site Green=Tags(s)
  
  X*AGLILEALGPLQAGSRLALQRRQQPHLLQGFLSPPLALEGLILGLPSNQPQVPLPPACSHLPPRLRPH
  RRQASLLEQRLLLRGELDFLWGSVLQSST*ATQLPPQPWQTPAALGWAAATSLMPYRAPSPPARAQHPPA
  LCLAPPPPLWLQLPHLEASHSLVEARPNPPVSTLAQQGIQPSPRHLPRCPSLRPRQQPPQQVPHSQLLPH
  PQPPSPVLGPASLRQ*QLLQPHLPPLDSPSVPL*PQRAPPLLGHRASA*RHLEQLPAPPQQHPPLPPPPP
  PPPAAAAPPALP*I*NHWRQPGSPAIQQLP*PLHLALAQLQGRLPAPP*PTRSWRA*STNGAWS*RTRSG
  TSSSRPPRSTPGTAR*SRMEKRSPACTARWRR*SWTRRGWTRSSTSSCPSRRSWKTC*AHWRSWSRSRAG
  PSTCSTRMRSVRKPTSWLRTSTHSSSAWPRISRTSSST*TRPGPPPTPVTHCSRSARSSMRTWTHCSGST
  RTRPCCRGRWRR*PRCARAGARSRSAASGSPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001193357
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001193357.1, NP_001180286.1
• RefSeq Size: 3200 bp
• RefSeq ORF: 1569 bp
• Locus ID: 23636
• Protein Families: Druggable Genome, Transcription Factors
• MW: 53.3 kDa
• Gene Summary: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq, Jul 2008]