A1CF (NM_001198820) Human Tagged ORF Clone

CAT#: RC234515

A1CF (Myc-DDK tagged) - Homo sapiens APOBEC1 complementation factor (A1CF), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: A1CF
• Synonyms: ACF; ACF64; ACF65; APOBEC1CF; ASP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234515 representing NM_001198820
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCAGTGTGTCTGGGCACATGCCCAGAGCCAGAAGCGAGCATGAGCACAGCAATTCCTGGCCTCA
  AAAAAGGAAATAATGCTTTGCAGTCCATCATTTTGCAAACTTTGCTGGAAAAGGAAAATGGACAAAGAAA
  ATATGGTGGCCCTCCACCTGGTTGGGATGCTGCACCCCCTGAAAGGGGCTGTGAAATTTTTATTGGAAAA
  CTTCCCCGAGACCTTTTTGAGGATGAGCTTATACCATTATGTGAAAAAATCGGTAAAATTTATGAAATGA
  GAATGATGATGGATTTTAATGGCAACAATAGAGGATATGCATTTGTAACATTTTCAAATAAAGTGGAAGC
  CAAGAATGCAATCAAGCAACTTAATAATTATGAAATTAGAAATGGGCGCCTCTTAGGGGTTTGTGCCAGT
  GTGGACAACTGCCGATTATTTGTTGGGGGCATCCCAAAAACCAAAAAGAGAGAAGAAATCTTATCGGAGA
  TGAAAAAGGTTACTGAAGGTGTTGTCGATGTCATCGTCTACCCAAGCGCTGCAGATAAAACCAAAAACCG
  AGGCTTTGCCTTCGTGGAGTATGAGAGTCATCGAGCAGCTGCCATGGCGAGGAGGAAACTGCTACCAGGA
  AGAATTCAGTTATGGGGACATGGTATTGCAGTAGACTGGGCAGAGCCAGAAGTAGAAGTTGATGAAGATA
  CAATGTCTTCAGTGAAAATCCTATATGTAAGAAATCTTATGCTGTCTACCTCTGAAGAGATGATTGAAAA
  GGAATTCAACAATATCAAACCAGGTGCTGTGGAGAGGGTGAAGAAAATTCGAGACTATGCTTTTGTGCAC
  TTCAGTAACCGAGAAGATGCAGTTGAGGCTATGAAAGCTTTAAATGGCAAGGTGCTGGATGGTTCCCCCA
  TTGAAGTCACCCTAGCAAAACCAGTGGACAAGGACAGTTATGTTAGGTATACCCGAGGCACAGGTGGAAG
  GGGCACCATGCTGCAAGGAGAGTATACCTACTCTTTGGGCCAAGTTTATGATCCCACCACAACCTACCTT
  GGAGCTCCTGTCTTCTATGCCCCCCAGACCTATGCAGCAATTCCCAGTCTTCATTTCCCAGCCACCAAAG
  GACATCTCAGCAACAGAGCCATTATCCGAGCCCCTTCTGTTAGAGGGGCTGCGGGAGTGAGAGGACTGGG
  CGGCCGTGGCTATTTGGCATACACAGGCCTGGGTCGAGGATACCAGGTCAAAGGAGACAAAAGAGAAGAC
  AAACTCTATGACATTTTACCTGGGATGGAGCTCACCCCAATGAATCCTGTCACATTAAAACCCCAAGGAA
  TTAAACTCGCTCCCCAGATATTAGAAGAGATTTGTCAGAAAAATAACTGGGGACAGCCAGTGTACCAGCT
  GCACTCTGCTATTGGACAAGACCAAAGACAGCTATTCTTGTACAAAATAACTATTCCTGCTCTAGCCAGC
  CAGAATCCTGCAATCCACCCTTTCACACCTCCAAAGCTGAGTGCCTTTGTGGATGAAGCAAAGACGTATG
  CAGCCGAATACACCCTGCAGACCCTGGGCATCCCCACTGATGGAGGCGATGGCACCATGGCTACTGCTGC
  TGCTGCTGCTACTGCTTTCCCAGGATATGCTGTCCCTAATGCAACTGCACCCGTGTCTGCAGCCCAGCTC
  AAGCAAGCGGTAACCCTTGGACAAGACTTAGCAGCATATACAACCTATGAGGTCTACCCAACTTTTGCAG
  TGACTGCCCGAGGGGATGGATATGGCACCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234515 representing NM_001198820
  Red=Cloning site Green=Tags(s)
  
  MEAVCLGTCPEPEASMSTAIPGLKKGNNALQSIILQTLLEKENGQRKYGGPPPGWDAAPPERGCEIFIGK
  LPRDLFEDELIPLCEKIGKIYEMRMMMDFNGNNRGYAFVTFSNKVEAKNAIKQLNNYEIRNGRLLGVCAS
  VDNCRLFVGGIPKTKKREEILSEMKKVTEGVVDVIVYPSAADKTKNRGFAFVEYESHRAAAMARRKLLPG
  RIQLWGHGIAVDWAEPEVEVDEDTMSSVKILYVRNLMLSTSEEMIEKEFNNIKPGAVERVKKIRDYAFVH
  FSNREDAVEAMKALNGKVLDGSPIEVTLAKPVDKDSYVRYTRGTGGRGTMLQGEYTYSLGQVYDPTTTYL
  GAPVFYAPQTYAAIPSLHFPATKGHLSNRAIIRAPSVRGAAGVRGLGGRGYLAYTGLGRGYQVKGDKRED
  KLYDILPGMELTPMNPVTLKPQGIKLAPQILEEICQKNNWGQPVYQLHSAIGQDQRQLFLYKITIPALAS
  QNPAIHPFTPPKLSAFVDEAKTYAAEYTLQTLGIPTDGGDGTMATAAAAATAFPGYAVPNATAPVSAAQL
  KQAVTLGQDLAAYTTYEVYPTFAVTARGDGYGTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198820
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198820.1, NP_001185749.1
• RefSeq Size: 9412 bp
• RefSeq ORF: 1785 bp
• Locus ID: 29974
• MW: 65 kDa
• Gene Summary: Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]