DYNC1I2 (NM_001271790) Human Tagged ORF Clone
CAT#: RC234562
- TrueORF®
DYNC1I2 (Myc-DDK tagged) - Homo sapiens dynein, cytoplasmic 1, intermediate chain 2 (DYNC1I2), transcript variant 7
"NM_001271790" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | DYNC1I2 |
Synonyms | DIC74; DNCI2; IC2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234562 representing NM_001271790
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGACAAAAGTGAATTAAAGGCTGAGTTGGAACGTAAGAAGCAGCGACTGGCCCAAATCAGAGAGG AAAAGAAGAGAAAAGAAGAAGAAAGGAAAAAAAAAGAAACAGACCAGAAGAAGGAAGCTGTTGCTCCTGT GCAAGAAGAATCAGATCTTGAAAAAAAAAGGAGAGAAGCTGAAGCATTGCTTCAAAGCATGGGGCTAACT CCAGAATCCCCCATTGTCCCTCCTCCTATGTCTCCATCCTCCAAATCTGTGAGCACTCCAAGTGAAGCTG GAAGCCAAGACTCTGGAGATGGCGCCGTGGGATCTAGACGAGGACCTATTAAACTTGGAATGGCTAAAAT CACGCAAGTCGACTTTCCTCCTCGAGAAATTGTCACGTATACAAAGGAAACTCAGACTCCAGTTATGGCT CAACCCAAAGAAGATGAAGAGGAAGATGATGATGTAGTGGCTCCTAAACCACCTATTGAACCTGAAGAAG AGAAAACTTTAAAGAAAGATGAGGAAAATGATAGTAAAGCTCCCCCTCATGAGCTGACTGAAGAAGAAAA GCAACAAATCTTGCACTCTGAGGAATTTTTAAGTTTCTTTGACCATTCTACAAGAATTGTAGAAAGAGCT CTTTCTGAGCAGATTAACATCTTCTTTGACTATAGTGGGAGAGATTTGGAAGACAAAGAAGGAGAGATTC AAGCAGGTGCTAAACTGTCATTAAATCGACAATTTTTTGACGAACGTTGGTCAAAGCATCGGGTGGTTAG TTGTTTGGATTGGTCATCTCAGTATCCGGAGTTACTCGTGGCTTCCTATAACAACAATGAAGATGCCCCT CATGAGCCTGATGGTGTGGCCCTTGTATGGAATATGAAATACAAAAAAACTACCCCAGAGTATGTGTTTC ACTGCCAGTCAGCTGTGATGTCTGCCACATTTGCAAAATTTCATCCAAATCTTGTTGTTGGTGGTACATA TTCAGGCCAAATTGTGCTTTGGGATAACCGTAGCAATAAAAGAACTCCAGTGCAAAGAACTCCACTGTCA GCAGCTGCACACACACACCCTGTATATTGTGTAAATGTTGTTGGAACACAAAATGCTCACAATCTGATTA GCATCTCTACTGATGGAAAAATTTGTTCATGGAGTCTGGACATGCTTTCCCATCCACAGGATAGCATGGA GTTGGTTCATAAACAGTCAAAAGCAGTAGCTGTGACATCTATGTCCTTCCCTGTTGGAGATGTCAACAAC TTTGTTGTTGGGAGTGAAGAAGGTTCTGTGTACACAGCATGCCGCCATGGCAGCAAAGCTGGAATCAGTG AGATGTTTGAGGGGCATCAAGGACCAATCACTGGCATCCATTGTCATGCAGCTGTTGGAGCAGTAGACTT CTCACATCTTTTTGTCACTTCATCGTTTGACTGGACAGTAAAGCTTTGGACAACTAAGAATAACAAGCCT TTGTATTCATTTGAAGATAATGCAGACTATGTTTATGATGTTATGTGGTCACCTACCCACCCAGCCCTGT TTGCCTGTGTGGATGGCATGGGGAGATTGGATTTGTGGAATCTCAATAATGACACAGAGGTACCAACTGC CAGCATTTCTGTGGAGGGTAATCCTGCTCTTAATCGTGTGAGATGGACCCATTCTGGCAGAGAGATTGCT GTGGGTGATTCTGAAGGACAGATTGTTATATACGATGTGGGAGAGATTGCTGTTCCCCGCAATGATGAAT GGGCACGGTTTGGCCGAACACTTGCAGAAATTAATGCAAACCGAGCTGATGCAGAGGAGGAAGCAGCTAC CCGAATACCTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234562 representing NM_001271790
Red=Cloning site Green=Tags(s) MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAVAPVQEESDLEKKRREAEALLQSMGLT PESPIVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRRGPIKLGMAKITQVDFPPREIVTYTKETQTPVMA QPKEDEEEDDDVVAPKPPIEPEEEKTLKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERA LSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEDAP HEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLS AAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNN FVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKP LYSFEDNADYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIA VGDSEGQIVIYDVGEIAVPRNDEWARFGRTLAEINANRADAEEEAATRIPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001271790 |
ORF Size | 1833 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001271790.1, NP_001258719.1 |
RefSeq Size | 4333 bp |
RefSeq ORF | 1836 bp |
Locus ID | 1781 |
Cytogenetics | 2q31.1 |
MW | 68.7 kDa |
Gene Summary | 'This gene encodes a member of the dynein intermediate chain family. The encoded protein is a non-catalytic component of the cytoplasmic dynein 1 complex, which acts as a retrograde microtubule motor to transport organelles and vesicles. A pseudogene of this gene is located on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]' |
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