DYNC1I2 (NM_001271789) Human Tagged ORF Clone

CAT#: RC234565

DYNC1I2 (Myc-DDK tagged) - Homo sapiens dynein, cytoplasmic 1, intermediate chain 2 (DYNC1I2), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DYNC1I2
• Synonyms: DIC74; DNCI2; IC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234565 representing NM_001271789
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGACAAAAGTGAATTAAAGGCTGAGTTGGAACGTAAGAAGCAGCGACTGGCCCAAATCAGAGAGG
  AAAAGAAGAGAAAAGAAGAAGAAAGGAAAAAAAAAGAAACAGACCAGAAGAAGGAAGCTGTTGCTCCTGT
  GCAAGAAGAATCAGATCTTGAAAAAAAAAGGAGAGAAGCTGAAGCATTGCTTCAAAGCATGGGGCTAACT
  CCAGAATCCCCCATTGTCCCTCCTCCTATGTCTCCATCCTCCAAATCTGTGAGCACTCCAAGTGAAGCTG
  GAAGCCAAGACTCTGGAGATGGCGCCGTGGGATCTAGACGAGGACCTATTAAACTTGGAATGGCTAAAAT
  CACGCAAGTCGACTTTCCTCCTCGAGAAATTGTCACGTATACAAAGGAAACTCAGACTCCAGTTATGGCT
  CAACCCAAAGAAGATGAAGAGGAAGATGATGATGTAGTGGCTCCTAAACCACCTATTGAACCTGAAGAAG
  AGAAAACTTTAAAGAAAGATGAGGAAAATGATAGTAAAGCTCCCCCTCATGAGCTGACTGAAGAAGAAAA
  GCAACAAATCTTGCACTCTGAGGAATTTTTAAGTTTCTTTGACCATTCTACAAGAATTGTAGAAAGAGCT
  CTTTCTGAGCAGATTAACATCTTCTTTGACTATAGTGGGAGAGATTTGGAAGACAAAGAAGGAGAGATTC
  AAGCAGGTGCTAAACTGTCATTAAATCGACAATTTTTTGACGAACGTTGGTCAAAGCATCGGGTGGTTAG
  TTGTTTGGATTGGTCATCTCAGTATCCGGAGTTACTCGTGGCTTCCTATAACAACAATGAAGATGCCCCT
  CATGAGCCTGATGGTGTGGCCCTTGTATGGAATATGAAATACAAAAAAACTACCCCAGAGTATGTGTTTC
  ACTGCCAGTCAGCTGTGATGTCTGCCACATTTGCAAAATTTCATCCAAATCTTGTTGTTGGTGGTACATA
  TTCAGGCCAAATTGTGCTTTGGGATAACCGTAGCAATAAAAGAACTCCAGTGCAAAGAACTCCACTGTCA
  GCAGCTGCACACACACACCCTGTATATTGTGTAAATGTTGTTGGAACACAAAATGCTCACAATCTGATTA
  GCATCTCTACTGATGGAAAAATTTGTTCATGGAGTCTGGACATGCTTTCCCATCCACAGGATAGCATGGA
  GTTGGTTCATAAACAGTCAAAAGCAGTAGCTGTGACATCTATGTCCTTCCCTGTTGGAGATGTCAACAAC
  TTTGTTGTTGGGAGTGAAGAAGGTTCTGTGTACACAGCATGCCGCCATGGCAGCAAAGCTGGAATCAGTG
  AGATGTTTGAGGGGCATCAAGGACCAATCACTGGCATCCATTGTCATGCAGCTGTTGGAGCAGTAGACTT
  CTCACATCTTTTTGTCACTTCATCGTTTGACTGGACAGTAAAGCTTTGGACAACTAAGAATAACAAGCCT
  TTGTATTCATTTGAAGATAATGCAGACTATGTTTATGATGTTATGTGGTCACCTACCCACCCAGCCCTGT
  TTGCCTGTGTGGATGGCATGGGGAGATTGGATTTGTGGAATCTCAATAATGACACAGAGGTACCAACTGC
  CAGCATTTCTGTGGAGGGTAATCCTGCTCTTAATCGTGTGAGATGGACCCATTCTGGCAGAGAGATTGCT
  GTGGGTGATTCTGAAGGACAGATTGTTATATACGATGTGGGAGAGCAGATTGCTGTTCCCCGCAATGATG
  AATGGGCACGGTTTGGCCGAACACTTGCAGAAATTAATGCAAACCGAGCTGATGCAGAGGAGGAAGCAGC
  TACCCGAATACCTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234565 representing NM_001271789
  Red=Cloning site Green=Tags(s)
  
  MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAVAPVQEESDLEKKRREAEALLQSMGLT
  PESPIVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRRGPIKLGMAKITQVDFPPREIVTYTKETQTPVMA
  QPKEDEEEDDDVVAPKPPIEPEEEKTLKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERA
  LSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEDAP
  HEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLS
  AAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNN
  FVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKP
  LYSFEDNADYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIA
  VGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINANRADAEEEAATRIPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271789
• ORF Size: 1836 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271789.1, NP_001258718.1
• RefSeq Size: 4319 bp
• RefSeq ORF: 1839 bp
• Locus ID: 1781
• Cytogenetics: 2q31.1
• MW: 68.9 kDa
• Gene Summary: 'This gene encodes a member of the dynein intermediate chain family. The encoded protein is a non-catalytic component of the cytoplasmic dynein 1 complex, which acts as a retrograde microtubule motor to transport organelles and vesicles. A pseudogene of this gene is located on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]'