PDLIM5 (NM_001256426) Human Tagged ORF Clone

CAT#: RC234597

PDLIM5 (Myc-DDK tagged) - Homo sapiens PDZ and LIM domain 5 (PDLIM5), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDLIM5
• Synonyms: ENH; ENH1; L9; LIM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234597 representing NM_001256426
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAACTACAGTGTGTCACTGGTTGGCCCAGCTCCTTGGGGTTTCCGGCTGCAGGGCGGTAAGGATT
  TCAACATGCCTCTGACAATCTCTAGTCTAAAAGATGGCGGCAAGGCAGCCCAGGCAAATGTAAGAATAGG
  CGATGTGGTTCTCAGCATTGATGGAATAAATGCACAAGGAATGACTCATCTTGAAGCCCAGAATAAGATT
  AAGGGTTGTACAGGCTCTTTGAATATGACTCTGCAAAGAGCATCTGCTGCACCCAAGCCTGAGCCGGTTC
  CTGTTCAAAAGCCCACAGTCACCAGCGTGTGTTCCGAGACTTCTCAGGAGCTAGCAGAGGGACAGAGAAG
  AGGATCCCAGGGTGACAGTAAACAGCAAAATGGGAAAATCCCACCTAAACGCCCACCAAGAAAACACATT
  GTGGAGCGCTATACAGAGTTTTATCATGTACCCACTCACAGTGATGCCAGCAAGAAGAGACTGATTGAGG
  ATACTGAAGACTGGCGTCCAAGGACTGGAACAACTCAGTCTCGCTCTTTCCGAATCCTTGCCCAGATCAC
  TGGGACTGAACATTTGAAAGAATCTGAAGCCGATAATACAAAGAAGGCAAAGTTTGACAGTGCTCTGGAA
  GACCTACCTAAGAGTGGACCTCACCCTCCTGCAACTCCTCAGGTGTTAACTATTGGTAGCCAAGTGGCCA
  CACTTTCTAAAGTAGCAACTACTTATTCTAGTTTAAGCAGCTCCACAGGAAATGTGGAAGATTCTTTCGA
  AGGTTTTCGAAACTTTTCTACCTTCTCTTCTCCTGCTAGATATAGTGCTGCAGTTCTGAGCAGCGCAGCT
  GCTACTGTGTCTGCTGTTATTGCTACAAAAACCAGGCTCTACACTCCTGAAAGATATCACTCACTCCTGG
  ATGCACTTTGCATCAGCCCTGTCTCCAAGCCTTTAGCTTTTTCCTATCTGCAGTCCTCAAGGAAATCAAC
  TGGCTCTATCCATGTTAAGAAAACAAGTAACTCTCAGGAGCCTTCTCCGCAGTTGGCTTCCTCGGTAGCT
  TCCACACGGAGCATGCCCGAGAGCCTGGACAGCCCAACCTCTGGCAGACCAGGGGTTACCAGCCTCACAA
  CTGCAGCTGCCTTCAAGCCTGTAGGATCCACTGGCGTCATCAAGTCACCAAGCTGGCAACGGCCAAACCA
  AGGAGTACCTTCCACTGGAAGAATCTCAAACAGCGCTACTTACTCAGGATCAGTGGCACCAGCCAACTCA
  GCTTTGGGACAAACCCAGCCAAGTGACCAGGACACTTTAGTGCAAAGAGCTGAGCACATTCCAGCAGGGA
  AACGAACTCCGATGTGCGCCCATTGTAACCAGGTCATCAGAGGACCATTCTTAGTGGCACTGGGGAAATC
  TTGGCACCCAGAAGAATTCAACTGCGCTCACTGCAAAAATACAATGGCCTACATTGGATTTGTAGAGGAG
  AAAGGAGCCCTGTATTGTGAGCTGTGCTATGAGAAATTCTTTGCCCCTGAATGTGGTCGATGCCAAAGGA
  AGATCCTTGGAGAAGTCATCAGTGCGTTGAAACAAACTTGGCATGTTTCCTGTTTTGTGTGTGTAGCCTG
  TGGAAAGCCCATTCGGAACAATGTTTTTCACTTGGAGGATGGTGAACCCTACTGTGAGACTGATTATTAT
  GCCCTCTTTGGTACTATATGCCATGGATGTGAATTTCCCATAGAAGCTGGTGACATGTTCCTGGAAGCTC
  TGGGCTACACCTGGCATGACACTTGCTTTGTATGCTCAGTGTGTTGTGAAAGTTTGGAAGGTCAGACCTT
  TTTCTCCAAGAAGGACAAGCCCCTGTGTAAGAAACATGCTCATTCTGTGAATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234597 representing NM_001256426
  Red=Cloning site Green=Tags(s)
  
  MSNYSVSLVGPAPWGFRLQGGKDFNMPLTISSLKDGGKAAQANVRIGDVVLSIDGINAQGMTHLEAQNKI
  KGCTGSLNMTLQRASAAPKPEPVPVQKPTVTSVCSETSQELAEGQRRGSQGDSKQQNGKIPPKRPPRKHI
  VERYTEFYHVPTHSDASKKRLIEDTEDWRPRTGTTQSRSFRILAQITGTEHLKESEADNTKKAKFDSALE
  DLPKSGPHPPATPQVLTIGSQVATLSKVATTYSSLSSSTGNVEDSFEGFRNFSTFSSPARYSAAVLSSAA
  ATVSAVIATKTRLYTPERYHSLLDALCISPVSKPLAFSYLQSSRKSTGSIHVKKTSNSQEPSPQLASSVA
  STRSMPESLDSPTSGRPGVTSLTTAAAFKPVGSTGVIKSPSWQRPNQGVPSTGRISNSATYSGSVAPANS
  ALGQTQPSDQDTLVQRAEHIPAGKRTPMCAHCNQVIRGPFLVALGKSWHPEEFNCAHCKNTMAYIGFVEE
  KGALYCELCYEKFFAPECGRCQRKILGEVISALKQTWHVSCFVCVACGKPIRNNVFHLEDGEPYCETDYY
  ALFGTICHGCEFPIEAGDMFLEALGYTWHDTCFVCSVCCESLEGQTFFSKKDKPLCKKHAHSVNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256426
• ORF Size: 1875 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001256426.1, NP_001243355.1
• RefSeq Size: 6219
• RefSeq ORF: 1878
• Locus ID: 10611
• Protein Families: Druggable Genome
• MW: 68.2 kDa
• Gene Summary: This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]