DIAPH3 (NM_001258370) Human Tagged ORF Clone

CAT#: RC234724

DIAPH3 (Myc-DDK tagged) - Homo sapiens diaphanous-related formin 3 (DIAPH3), transcript variant 7

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DIAPH3
• Synonyms: AN; AUNA1; DIA2; diap3; DRF3; mDia2; NSDAN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234724 representing NM_001258370
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAGGAGAGGAGCCTTTCCTTATTGGCCAAAGCCGTGGATCCCAGACACCCCAATATGATGACAG
  ATGTGGTTAAACTTCTCTCTGCGGTATGCATTGTAGGGGAAGAAAGCATCCTTGAAGAAGTTTTAGAAGC
  TTTAACTTCAGCTGGTGAAGAAAAAAAAATTGACAGATTTTTTTGTATTGTGGAAGGCCTCCGGCACAAT
  TCAGTTCAACTGCAAGTAGCTTGTATGCAGCTCATCAATGCCCTGGTTACATCTCCTGATGATTTGGATT
  TCAGGCTTCACATCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAATTTAAAATGCAT
  TAAGAATGATGGCCTGGATATCCAACTTAAAGTCTTTGATGAGCATAAAGAAGAAGATTTGTTTGAGTTA
  TCCCATCGCCTTGAAGATATTAGAGCTGAACTTGATGAAGCATATGATGTTTACAACATGGTGTGGAGCA
  CAGTTAAAGAAACTAGAGCAGAGGGATATTTTATTTCTATTCTTCAGCATCTTTTGCTGATTCGAAATGA
  TTATTTTATAAGGCAACAATACTTCAAATTAATTGATGAGTGTGTATCCCAGATTGTATTGCATAGAGAT
  GGAATGGATCCAGACTTCACATATCGAAAAAGACTAGATTTAGATTTAACCCAGTTTGTAGACATTTGCA
  TAGATCAAGCAAAACTAGAAGAGTTTGAAGAGAAAGCATCAGAACTTTACAAGAAATTTGAAAAAGAGTT
  TACCGACCACCAAGAAACTCAGGCTGAATTGCAGAAAAAAGAGGCAAAGATTAATGAGCTTCAAGCAGAG
  CTACAAGCTTTTAAGTCTCAGTTTGGTGCCTTGCCAGCTGATTGTAATATTCCTTTGCCTCCCTCTAAAG
  AAGGTGGAACTGGCCACTCAGCACTTCCTCCTCCGCCTCCACTGCCTTCTGGTGGAGGGGTGCCGCCTCC
  ACCTCCTCCCCCACCACCTCCTCCACTTCCAGGAATGCGGATGCCATTCAGTGGTCCTGTGCCTCCACCA
  CCTCCCCTGGGATTCCTTGGAGGACAAAATTCTCCTCCTCTACCAATCCTGCCATTTGGGTTGAAACCAA
  AGAAAGAATTTAAACCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATCAGACCTCATGAAATGAC
  TGAAAACTGTTTCTGGATAAAAGTAAATGAAAATAAGTATGAAAACGTGGATTTGCTTTGTAAACTTGAG
  AATACATTTTGTTGCCAACAAAAAGAGAGAAGAGAAGAGGAAGATATTGAAGAGAAGAAATCGATTAAGA
  AAAAAATTAAAGAACTTAAGTTTTTAGATTCTAAAATTGCCCAGAACCTTTCAATCTTCCTGAGCTCTTT
  TCGGGTGCCATATGAGGAAATCAGAATGATGATATTGGAAGTAGATGAAACACGGTTGGCAGAGTCTATG
  ATTCAGAACTTAATAAAGCATCTTCCTGATCAAGAGCAATTAAATTCATTGTCTCAGTTCAAGAGTGAAT
  ATAGCAACTTATGTGAACCTGAGCAGTTTGTGGTTGTGATGAGCAATGTGAAGAGACTACGGCCACGGCT
  CAGTGCTATTCTCTTTAAGCTTCAGTTTGAAGAGCAGGTGAACAACATCAAACCTGACATCATGGCTGTC
  AGTACTGCCTGCGAAGAGATAAAGAAGAGCAAAAGCTTTAGCAAGTTGCTGGAACTTGTATTGCTAATGG
  GAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTAACCTTAGCTCTCTCTGTAAACT
  AAAGGACACAAAATCAGCAGATCAGAAAACAACGCTACTTCATTTCCTGGTAGAAATATGTGAAGAGAAG
  TACCCTGATATACTGAATTTTGTGGATGATTTGGAACCTTTAGACAAAGCTAGTAAAGGTTTGTGCCTTT
  TTAAGAAACATTTCATGGCTCTGATCTTCAGTGCTAAAAGACTGAAAATTATACCTTTTATTTGTATGTA
  CTTTCCACTTTCTCACAGTGTTTTCATACCCAATATCTCATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234724 representing NM_001258370
  Red=Cloning site Green=Tags(s)
  
  MSEERSLSLLAKAVDPRHPNMMTDVVKLLSAVCIVGEESILEEVLEALTSAGEEKKIDRFFCIVEGLRHN
  SVQLQVACMQLINALVTSPDDLDFRLHIRNEFMRCGLKEILPNLKCIKNDGLDIQLKVFDEHKEEDLFEL
  SHRLEDIRAELDEAYDVYNMVWSTVKETRAEGYFISILQHLLLIRNDYFIRQQYFKLIDECVSQIVLHRD
  GMDPDFTYRKRLDLDLTQFVDICIDQAKLEEFEEKASELYKKFEKEFTDHQETQAELQKKEAKINELQAE
  LQAFKSQFGALPADCNIPLPPSKEGGTGHSALPPPPPLPSGGGVPPPPPPPPPPPLPGMRMPFSGPVPPP
  PPLGFLGGQNSPPLPILPFGLKPKKEFKPEISMRRLNWLKIRPHEMTENCFWIKVNENKYENVDLLCKLE
  NTFCCQQKERREEEDIEEKKSIKKKIKELKFLDSKIAQNLSIFLSSFRVPYEEIRMMILEVDETRLAESM
  IQNLIKHLPDQEQLNSLSQFKSEYSNLCEPEQFVVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAV
  STACEEIKKSKSFSKLLELVLLMGNYMNAGSRNAQTFGFNLSSLCKLKDTKSADQKTTLLHFLVEICEEK
  YPDILNFVDDLEPLDKASKGLCLFKKHFMALIFSAKRLKIIPFICMYFPLSHSVFIPNISF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258370
• ORF Size: 2073 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001258370.1, NP_001245299.1
• RefSeq Size: 2300
• RefSeq ORF: 2076
• Locus ID: 81624
• Protein Pathways: Regulation of actin cytoskeleton
• MW: 79.9 kDa
• Gene Summary: This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]