SEMA4G (NM_001203244) Human Tagged ORF Clone

CAT#: RC234743

SEMA4G (Myc-DDK tagged) - Homo sapiens sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G (SEMA4G), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEMA4G
• Synonyms: FLJ20590; KIAA1619; MGC102867
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234743 representing NM_001203244
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGGGAGGCTCTGGCCCCTCCTCCTCAGCATCCTCACAGCAACTGCAGTCCCAGGACCCTCACTGC
  GGAGACCGTCTAGAGAACTAGATGCCACCCCTCGGATGACCATACCCTATGAAGAGCTCTCTGGGACCCG
  GCACTTCAAGGGCCAAGCCCAGAACTACTCAACACTGCTGCTGGAGGAGGCCTCAGCAAGGCTGCTGGTG
  GGAGCCCGAGGTGCCCTGTTCTCTCTCAGTGCCAACGACATAGGAGATGGGGCTCACAAAGAGATCCACT
  GGGAAGCCTCCCCAGAGATGCAAAGCAAATGTCATCAAAAAGGGAAAAACAACCAGACGGAGTGCTTTAA
  CCATGTGCGGTTCCTGCAGCGGCTCAATTCTACCCACCTCTATGCATGTGGGACTCACGCCTTCCAGCCC
  CTCTGTGCAGCCATTGATGCTGAGGCCTTCACCTTGCCAACCAGCTTCGAGGAGGGGAAGGAGAAGTGTC
  CTTATGACCCAGCCCGTGGCTTCACAGGCCTCATCATTGATGGAGGCCTCTACACAGCCACTAGGTATGA
  ATTCCGGAGCATTCCTGACATCCGCCGGAGCCGCCACCCACACTCCCTGAGAACTGAGGAGACACCAATG
  CATTGGCTCAATGATGCGGAGTTTGTGTTCTCCGTCCTCGTGCGGGAGAGCAAGGCCAGTGCAGTGGGTG
  ATGATGACAAGGTGTACTACTTCTTCACGGAGCGTGCCACTGAGGAGGGCTCTGGCAGCTTCACTCAGAG
  CCGCAGCAGTCACCGTGTGGCCCGTGTGGCTCGTGTCTGCAAGGGAGACCTGGGAGGGAAGAAGATCCTG
  CAGAAGAAGTGGACTTCCTTCCTGAAAGCCCGTCTCATCTGCCACATTCCACTGTATGAGACACTGCGTG
  GGGTCTGCAGCCTGGATGCTGAAACCTCAAGCCGTACACACTTCTATGCAGCCTTCACGCTGAGCACACA
  GTGGAAGACCCTGGAGGCCTCAGCCATCTGCCGCTATGACCTGGCAGAGATCCAGGCTGTCTTTGCAGGA
  CCCTATATGGAATACCAGGATGGTTCCCGGCGCTGGGGTCGCTATGAGGGTGGGGTGCCTGAGCCCCGGC
  CTGGCTCGTGTATCACAGATTCATTGCGCAGCCAAGGCTACAATTCATCCCAAGACTTGCCATCCCTGGT
  CCTGGACTTTGTAAAGTTGCACCCACTGATGGCTCGGCCCGTTGTGCCCACACGTGGACGGCCCCTGCTG
  CTCAAGCGCAACATACGCTACACACACCTTACAGGGACACCTGTCACCACGCCTGCTGGACCTACCTATG
  ACCTGCTCTTTCTGGGCACAGCTGATGGCTGGATCCACAAGGCCGTAGTCCTGGGCTCTGGGATGCACAT
  TATTGAAGAGACACAAGTGTTCAGGGAGTCCCAGTCTGTGGAAAATCTAGTCATCTCTCTATTGCAGCAC
  AGCCTCTATGTGGGGGCTCCTAGCGGAGTCATCCAGCTACCACTCTCCAGCTGCTCCCGCTACCGATCCT
  GCTATGACTGCATCTTGGCCCGAGACCCCTACTGTGGCTGGGACCCTGGCACCCATGCCTGCGCAGCAGC
  CACCACCATAGCCAACAGGTCCCAGGGAAGCAGGACAGCACTGATACAGGACATAGAGAGAGGAAATCGA
  GGCTGTGAGAGCAGCAGGGATACAGGCAGGGCTCTGCAGGTCCATATGGGCTCAATGTCACCACCCTCTG
  CATGGCCCTGTGTGCTGGATGGTCCTGAAACCAGACAAGACCTCTGCCAGCCACCTAAGCCCTGCGTACA
  TTCACATGCACACATGGAAGAATGTTTATCGGCTGGGCTGCAGTGCCCCCACCCTCACCTTCTCCTGGTG
  CATTCTTGTTTCATCCCTGCTTCTGGACTTGGGGTACCCTCCCAATTGCCACATCCTATCTGGTCCTCTT
  CCCCAGCCCCATGTGGTGACCTCTTTGTCAAGAGCTTGGGAACGGGCCAGCCTGGGGAGGTAAGACTGCA
  TCACTCCCCTCCTCTCCCTTCCTGTGTGGCCCTTGTGAATCAGCCTCCCCACTCTCCTTGGTCATTCTCA
  AGAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234743 representing NM_001203244
  Red=Cloning site Green=Tags(s)
  
  MWGRLWPLLLSILTATAVPGPSLRRPSRELDATPRMTIPYEELSGTRHFKGQAQNYSTLLLEEASARLLV
  GARGALFSLSANDIGDGAHKEIHWEASPEMQSKCHQKGKNNQTECFNHVRFLQRLNSTHLYACGTHAFQP
  LCAAIDAEAFTLPTSFEEGKEKCPYDPARGFTGLIIDGGLYTATRYEFRSIPDIRRSRHPHSLRTEETPM
  HWLNDAEFVFSVLVRESKASAVGDDDKVYYFFTERATEEGSGSFTQSRSSHRVARVARVCKGDLGGKKIL
  QKKWTSFLKARLICHIPLYETLRGVCSLDAETSSRTHFYAAFTLSTQWKTLEASAICRYDLAEIQAVFAG
  PYMEYQDGSRRWGRYEGGVPEPRPGSCITDSLRSQGYNSSQDLPSLVLDFVKLHPLMARPVVPTRGRPLL
  LKRNIRYTHLTGTPVTTPAGPTYDLLFLGTADGWIHKAVVLGSGMHIIEETQVFRESQSVENLVISLLQH
  SLYVGAPSGVIQLPLSSCSRYRSCYDCILARDPYCGWDPGTHACAAATTIANRSQGSRTALIQDIERGNR
  GCESSRDTGRALQVHMGSMSPPSAWPCVLDGPETRQDLCQPPKPCVHSHAHMEECLSAGLQCPHPHLLLV
  HSCFIPASGLGVPSQLPHPIWSSSPAPCGDLFVKSLGTGQPGEVRLHHSPPLPSCVALVNQPPHSPWSFS
  RV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001203244
• ORF Size: 2106 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001203244.1, NP_001190173.1
• RefSeq Size: 3230
• RefSeq ORF: 2109
• Locus ID: 57715
• Protein Families: Transmembrane
• Protein Pathways: Axon guidance
• MW: 78 kDa
• Gene Summary: Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]