CPT1C (NM_001199753) Human Tagged ORF Clone

CAT#: RC234902

CPT1C (Myc-DDK tagged) - Homo sapiens carnitine palmitoyltransferase 1C (CPT1C), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPT1C
• Synonyms: CATL1; CPT1-B; CPT1P; CPTI-B; CPTIC; SPG73
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234902 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGGCTGAAGCGCACCAGGCCGTGGGCTTCCGACCCTCGCTGACCTCGGACGGGGCTGAAGTGGAACT
  CAGTGCCCCTGTGCTGCAGGAGATCTACCTCTCTGGCCTGCGCTCCTGGAAAAGGCATCTCTCACGTTTC
  TGGAATGACTTTCTCACCGGTGTGTTTCCTGCCAGCCCCCTCAGTTGGCTTTTCCTCTTCAGTGCCATCC
  AGCTTGCCTGGTTCCTCCAGCTGGATCCTTCCTTAGGACTGATGGAGAAGATCAAAGAGTTGCTGCCTGA
  CTGGGGTGGACAACACCACGGGCTCCGGGGGGTCCTGGCAGCCGCGCTGTTTGCCTCGTGTTTGTGGGGA
  GCCCTGATCTTCACACTGCACGTGGCCCTGAGGCTGCTTCTGTCCTACCACGGCTGGCTTCTTGAGCCCC
  ACGGAGCCATGTCCTCCCCCACCAAGACCTGGCTGGCCCTGGTCCGCATCTTCTCTGGCCGCCACCCGAT
  GCTGTTCAGTTACCAGCGCTCCCTGCCACGCCAGCCCGTGCCCTCTGTGCAGGACACCGTGCGCAAGTAC
  CTGGAGTCGGTCCGGCCCATCCTCTCCGACGAGGACTTCGACTGGACCGCGGTCCTGGCGCAGGAATTCC
  TGAGGCTGCAGGCGTCGCTGCTGCAGTGGTACCTGCGGCTCAAGTCCTGGTGGGCGTCCAATTATGTCAG
  TGACTGGTGGGAGGAATTTGTGTACCTGCGCTCCCGAAATCCGCTGATGGTGAACAGCAACTATTACATG
  ATGGACTTCCTGTATGTCACACCCACGCCTCTGCAGGCAGCTCGCGCTGGGAATGCCGTCCATGCCCTCC
  TCCTGTACCGCCACCGCCTGAACCGCCAGGAGATACCCCCGACTTTGCTGATGGGAATGCGCCCCTTATG
  CTCTGCCCAGTACGAGAAGATCTTCAACACCACGCGGATTCCAGGGGTCCAAAAAGACTACATCCGCCAC
  CTCCATGACAGCCAACACGTGGCTGTCTTCCACCGGGGCCGATTCTTCCGCATGGGGACCCACTCCCGAA
  ACAGCCTGCTTTCCCCGAGAGCCCTGGAGCAGCAGTTTCAGAGAATCCTGGATGATCCCTCACCGGCCTG
  CCCCCACGAGGAACATCTGGCAGCTCTGACAGCTGCTCCCAGGGGCACGTGGGCCCAGGTGCGGACATCC
  CTGAAGACCCAGGCAGCGGAGGCCCTGGAGGCGGTGGAAGGGGCCGCTTTCTTTGTGTCACTGGATGCTG
  AGCCCGCGGGGCTCACCAGGGAGGACCCGGCAGCGTCGTTGGATGCCTACGCCCATGCTCTGCTGGCCGG
  CCGGGGCCATGATCGCTGGTTTGACAAATCCTTCACCCTAATCGTCTTCTCTAACGGGAAGCTGGGCCTC
  AGCGTGGAGCACTCCTGGGCCGACTGCCCCATCTCAGGACACATGTGGGAGTTCACTCTGGCTACAGAAT
  GCTTTCAGCTGGGCTACTCAACAGACGGCCACTGCAAGGGGCACCCGGACCCCACACTACCCCAGCCCCA
  GCGGCTGCAATGGGACCTTCCAGACCAGATCCACTCCTCCATCTCTCTAGCCCTGAGGGGAGCCAAGATC
  TTGTCTGAAAATGTCGACTGCCATGTCGTTCCATTCTCCCTATTTGGCAAGAGCTTCATCCGACGCTGCC
  ACCTCTCTTCAGACAGCTTCATCCAGATCGCCTTGCAACTGGCCCACTTCCGGGACAGGGGTCAATTCTG
  CCTGACTTATGAGTCGGCCATGACTCGCTTATTCCTGGAAGGCCGGACGGAGACGGTGCGGTCTTGCACG
  AGGGAGGCCTGCAACTTTGTCAGGGCCATGGAGGACAAAGAGAAGACGGACCCACAGTGCCTCGCCCTGT
  TCCGCGTGGCAGTGGACAAGCACCAGGCTCTGCTGAAGGCAGCCATGAGCGGGCAGGGAGTTGACCGCCA
  CCTGTTTGCGCTGTACATCGTGTCCCGATTCCTCCACCTGCAGTCGCCCTTCCTGACCCAGGTCCATTCG
  GAGCAGTGGCAGCTGTCCACCAGCCAGATCCCTGTTCAGCAAATGCATCTGTTTGACGTCCACAATTACC
  CGGACTATGTTTCCTCAGGCGGTGGATTCGGGCCTGCTGATGACCATGGTTATGGTGTTTCTTATATCTT
  CATGGGGGATGGCATGATCACCTTCCACATCTCCAGCAAAAAATCAAGCACAAAAACGGATTCCCACAGG
  CTGGGGCAGCACATTGAGGACGCACTGCTGGATGTGGCCTCCCTGTTCCAGGCGGGACAGCATTTTAAGC
  GCCGGTTCAGAGGGTCAGGGAAGGAGAACTCCAGGCACAGGTGTGGATTTCTCTCCCGCCAGACTGGGGC
  CTCCAAGGCCTCAATGACATCCACCGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234902 protein sequence
  Red=Cloning site Green=Tags(s)
  
  XWLKRTRPWASDPR*PRTGLKWNSVPLCCRRSTSLACAPGKGISHVSGMTFSPVCFLPAPSVGFSSSVPS
  SLPGSSSWILP*D*WRRSKSCCLTGVDNTTGSGGSWQPRCLPRVCGEP*SSHCTWP*GCFCPTTAGFLSP
  TEPCPPPPRPGWPWSASSLAATRCCSVTSAPCHASPCPLCRTPCASTWSRSGPSSPTRTSTGPRSWRRNS
  *GCRRRCCSGTCGSSPGGRPIMSVTGGRNLCTCAPEIR*W*TATIT*WTSCMSHPRLCRQLALGMPSMPS
  SCTATA*TARRYPRLC*WECAPYALPSTRRSSTPRGFQGSKKTTSATSMTANTWLSSTGADSSAWGPTPE
  TACFPREPWSSSFRESWMIPHRPAPTRNIWQL*QLLPGARGPRCGHP*RPRQRRPWRRWKGPLSLCHWML
  SPRGSPGRTRQRRWMPTPMLCWPAGAMIAGLTNPSP*SSSLTGSWASAWSTPGPTAPSQDTCGSSLWLQN
  AFSWATQQTATARGTRTPHYPSPSGCNGTFQTRSTPPSL*P*GEPRSCLKMSTAMSFHSPYLARASSDAA
  TSLQTASSRSPCNWPTSGTGVNSA*LMSRP*LAYSWKAGRRRCGLARGRPATLSGPWRTKRRRTHSASPC
  SAWQWTSTRLC*RQP*AGRELTATCLRCTSCPDSSTCSRPS*PRSIRSSGSCPPARSLFSKCICLTSTIT
  RTMFPQAVDSGLLMTMVMVFLISSWGMA*SPSTSPAKNQAQKRIPTGWGSTLRTHCWMWPPCSRRDSILS
  AGSEGQGRRTPGTGVDFSPARLGPPRPQ*HPPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199753
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199753.1, NP_001186682.1
• RefSeq Size: 2883 bp
• RefSeq ORF: 2412 bp
• Locus ID: 126129
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, PPAR signaling pathway
• MW: 91 kDa
• Gene Summary: This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein regulates the beta-oxidation and transport of long-chain fatty acids into mitochondria, and may play a role in the regulation of feeding behavior and whole-body energy homeostasis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]