ADCY5 (NM_001199642) Human Tagged ORF Clone

CAT#: RC235022

ADCY5 (Myc-DDK tagged) - Homo sapiens adenylate cyclase 5 (ADCY5), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADCY5
• Synonyms: AC5; FDFM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235022 representing NM_001199642
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTCTCAGAAAGAGGGCTGTTGCAGCAGAGGTGACCTGTCCATTCAGACAGGTCCTGGAGGAGAAT
  GGGCCCCACGGAGGCTTGTCTCCAATGTTCTCATTTTCTCCTGCACCAACATCGTGGGTGTCTGCACCCA
  CTATCCGGCTGAGGTCTCCCAGAGACAGGCTTTCCAGGAGACCCGAGAGTGCATCCAGGCGCGGCTCCAC
  TCGCAGCGGGAGAACCAGCAGCAGGAACGGCTCCTGCTGTCTGTCCTTCCCCGTCATGTTGCCATGGAGA
  TGAAAGCAGACATCAACGCCAAGCAGGAGGATATGATGTTCCATAAGATTTACATCCAGAAACATGACAA
  CGTGAGCATCCTGTTTGCTGACATCGAGGGCTTCACCAGCCTGGCGTCCCAGTGCACTGCACAGGAACTG
  GTCATGACCCTCAACGAGCTCTTCGCCCGCTTTGACAAGCTGGCCGCAGAGAATCACTGTTTACGTATTA
  AGATCCTTGGGGATTGTTATTACTGCGTCTCGGGGCTGCCTGAAGCAAGGGCTGACCACGCCCACTGCTG
  TGTGGAGATGGGCATGGACATGATCGAGGCCATCTCGTTGGTCCGGGAGGTGACAGGGGTGAACGTGAAC
  ATGCGTGTGGGAATTCACAGCGGGCGAGTACACTGCGGTGTCCTTGGTCTCAGGAAGTGGCAGTTCGACG
  TCTGGTCTAACGATGTCACGCTAGCCAACCACATGGAGGCTGGCGGCAAGGCAGGACGCATCCACATCAC
  CAAGGCTACACTCAACTACCTGAATGGGGACTACGAGGTGGAGCCAGGCTGTGGGGGCGAGCGCAACGCC
  TACCTCAAGGAGCACAGTATCGAGACCTTCCTCATCCTGCGCTGCACCCAGAAGCGGAAAGAAGAGAAGG
  CCATGATCGCCAAGATGAACCGCCAGAGAACCAACTCCATCGGGCACAACCCACCACACTGGGGGGCTGA
  GCGCCCCTTCTACAACCACCTGGGTGGCAACCAGGTGTCCAAGGAGATGAAGCGGATGGGCTTTGAAGAC
  CCCAAGGACAAGAACGCCCAGGAGAGTGCGAACCCTGAGGATGAAGTGGATGAGTTTCTGGGCCGTGCCA
  TTGACGCCAGGAGCATTGATAGGCTTCGGTCTGAGCACGTCCGCAAGTTCCTCCTGACCTTCAGGGAGCC
  TGACTTAGAGAAGAAGTACTCCAAGCAGGTAGACGACCGATTTGGTGCCTATGTGGCGTGTGCCTCGCTC
  GTCTTCCTCTTCATCTGCTTTGTCCAGATCACCATCGTGCCCCACTCCATATTCATGCTCAGCTTCTACC
  TGACCTGTTCCCTGCTGCTGACCTTGGTGGTGTTTGTGTCTGTGATCTACTCCTGCGTAAAGCTCTTCCC
  CTCCCCACTGCAGACCCTCTCCAGGAAGATCGTGCGGTCCAAGATGAACAGCACCCTGGTTGGGGTGTTC
  ACCATCACCCTGGTGTTCCTGGCGGCTTTTGTCAACATGTTCACGTGCAACTCCAGGGACCTGCTGGGCT
  GCTTGGCACAGGAGCACAACATCAGCGCGAGCCAGGTCAACGCGTGTCACGTGGCGGAGTCGGCCGTCAA
  CTACAGCCTGGGCGATGAGCAGGGCTTCTGTGGCAGCCCCTGGCCCAACTGCAACTTCCCCGAGTACTTC
  ACCTACAGCGTGCTGCTCAGCCTGCTGGCCTGCTCCGTGTTCCTGCAGATCAGCTGCATCGGGAAGCTGG
  TGCTCATGCTGGCCATCGAGCTCATCTACGTGCTCATCGTGGAGGTGCCAGGTGTCACGCTCTTCGACAA
  CGCCGACCTGCTGGTCACCGCCAACGCCATAGACTTCTTCAACAACGGGACCTCCCAGTGCCCTGAGCAT
  GCAACCAAGGTGGCATTGAAGGTGGTGACGCCCATCATCATCTCAGTCTTTGTGCTGGCCCTGTACCTGC
  ACGCCCAGCAGGTGGAGTCCACTGCCCGCCTCGACTTCCTCTGGAAACTGCAGGCCACAGAGGAGAAAGA
  GGAGATGGAGGAGCTGCAGGCCTACAACCGGCGGCTGCTGCACAACATCCTGCCCAAGGACGTGGCCGCT
  CACTTCCTGGCCCGCGAGCGGCGCAATGATGAGCTCTACTATCAGTCCTGTGAGTGTGTGGCGGTCATGT
  TCGCCTCCATCGCCAACTTCTCCGAGTTCTACGTTGAGCTGGAGGCCAACAACGAGGGTGTCGAGTGCCT
  GCGGCTACTCAATGAGATCATCGCTGACTTTGATGAGATCATCAGCGAGGATCGGTTCCGGCAGCTGGAG
  AAGATCAAGACCATCGGCAGCACCTACATGGCTGCCTCCGGCCTCAACGACTCTACCTACGACAAGGTGG
  GCAAGACCCACATCAAGGCACTGGCCGACTTTGCCATGAAGCTGATGGACCAGATGAAGTACATCAATGA
  GCACTCCTTCAACAACTTCCAGATGAAGATCGGGCTCAACATCGGCCCCGTGGTGGCCGGGGTGATAGGG
  GCACGAAAGCCTCAGTACGACATCTGGGGCAATACCGTGAACGTGGCCAGCCGCATGGACAGCACCGGTG
  TACCCGACCGCATCCAGGTCACCACAGACATGTACCAGGTGCTGGCTGCCAACACGTACCAGCTGGAGTG
  CCGGGGCGTGGTCAAGGTCAAGGGCAAAGGCGAGATGATGACCTACTTCCTCAATGGAGGGCCCCCGCTC
  AGT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235022 representing NM_001199642
  Red=Cloning site Green=Tags(s)
  
  MKSQKEGCCSRGDLSIQTGPGGEWAPRRLVSNVLIFSCTNIVGVCTHYPAEVSQRQAFQETRECIQARLH
  SQRENQQQERLLLSVLPRHVAMEMKADINAKQEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQEL
  VMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGMDMIEAISLVREVTGVNVN
  MRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGKAGRIHITKATLNYLNGDYEVEPGCGGERNA
  YLKEHSIETFLILRCTQKRKEEKAMIAKMNRQRTNSIGHNPPHWGAERPFYNHLGGNQVSKEMKRMGFED
  PKDKNAQESANPEDEVDEFLGRAIDARSIDRLRSEHVRKFLLTFREPDLEKKYSKQVDDRFGAYVACASL
  VFLFICFVQITIVPHSIFMLSFYLTCSLLLTLVVFVSVIYSCVKLFPSPLQTLSRKIVRSKMNSTLVGVF
  TITLVFLAAFVNMFTCNSRDLLGCLAQEHNISASQVNACHVAESAVNYSLGDEQGFCGSPWPNCNFPEYF
  TYSVLLSLLACSVFLQISCIGKLVLMLAIELIYVLIVEVPGVTLFDNADLLVTANAIDFFNNGTSQCPEH
  ATKVALKVVTPIIISVFVLALYLHAQQVESTARLDFLWKLQATEEKEEMEELQAYNRRLLHNILPKDVAA
  HFLARERRNDELYYQSCECVAVMFASIANFSEFYVELEANNEGVECLRLLNEIIADFDEIISEDRFRQLE
  KIKTIGSTYMAASGLNDSTYDKVGKTHIKALADFAMKLMDQMKYINEHSFNNFQMKIGLNIGPVVAGVIG
  ARKPQYDIWGNTVNVASRMDSTGVPDRIQVTTDMYQVLAANTYQLECRGVVKVKGKGEMMTYFLNGGPPL
  S
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199642
• ORF Size: 2733 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199642.1, NP_001186571.1
• RefSeq Size: 5123 bp
• RefSeq ORF: 2736 bp
• Locus ID: 111
• Cytogenetics: 3q21.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Vascular smooth muscle contraction
• MW: 103.1 kDa
• Gene Summary: 'This gene encodes a member of the membrane-bound adenylyl cyclase enzymes. Adenylyl cyclases mediate G protein-coupled receptor signaling through the synthesis of the second messenger cAMP. Activity of the encoded protein is stimulated by the Gs alpha subunit of G protein-coupled receptors and is inhibited by protein kinase A, calcium and Gi alpha subunits. Single nucleotide polymorphisms in this gene may be associated with low birth weight and type 2 diabetes. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]'