HIP1 (NM_001243198) Human Tagged ORF Clone
CAT#: RC235103
- TrueORF®
HIP1 (Myc-DDK tagged) - Homo sapiens huntingtin interacting protein 1 (HIP1), transcript variant 2
"NM_001243198" in other vectors (2)
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Other products for "HIP1"
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | HIP1 |
Synonyms | HIP-I; ILWEQ; SHON; SHONbeta; SHONgamma |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC235103 representing NM_001243198
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGGATGGCCAGCTCCATGAAGCAGGTGCCCAACCCACTGCCCAAGGTGCTGAGCCGGCGCGGGG TCGGCGCTGGGCTGGAGGCGGCGGAGCGCGAGAGCTTCGAGCGGACTCAGACTGTCAGCATCAATAAGGC CATTAATACGCAGGAAGTGGCTGTAAAGGAAAAACACGCCAGAACGTGCATACTGGGCACCCACCATGAG AAAGGGGCACAGACCTTCTGGTCTGTTGTCAACCGCCTGCCTCTGTCTAGCAACGCAGTGCTCTGCTGGA AGTTCTGCCATGTGTTCCACAAACTCCTCCGAGATGGACACCCGAACGTCCTGAAGGACTCTCTGAGATA CAGAAATGAATTGAGTGACATGAGCAGGATGTGGGGCCACCTGAGCGAGGGGTATGGCCAGCTGTGCAGC ATCTACCTGAAACTGCTAAGAACCAAGATGGAGTACCACACCAAAAATCCCAGGTTCCCAGGCAACCTGC AGATGAGTGACCGCCAGCTGGACGAGGCTGGAGAAAGTGACGTGAACAACTTTTTCCAGTTAACAGTGGA GATGTTTGACTACCTGGAGTGTGAACTCAACCTCTTCCAAACAGTATTCAACTCCCTGGACATGTCCCGC TCTGTGTCCGTGACGGCAGCAGGGCAGTGCCGCCTCGCCCCGCTGATCCAGGTCATCTTGGACTGCAGCC ACCTTTATGACTACACTGTCAAGCTTCTCTTCAAACTCCACTCCTGCCTCCCAGCTGACACCCTGCAAGG CCACCGGGACCGCTTCATGGAGCAGTTTACAAAGTTGAAAGATCTGTTCTACCGCTCCAGCAACCTGCAG TACTTCAAGCGGCTCATTCAGATCCCCCAGCTGCCTGAGAACCCACCCAACTTCCTGCGAGCCTCAGCCC TGTCAGAACATATCAGCCCTGTGGTGGTGATCCCTGCAGAGGCCTCATCCCCCGACAGCGAGCCAGTCCT AGAGAAGGATGACCTCATGGACATGGATGCCTCTCAGCAGAATTTATTTGACAACAAGTTTGATGACATC TTTGGCAGTTCATTCAGCAGTGATCCCTTCAATTTCAACAGTCAAAATGGTGTGAACAAGGATGAGAAGG ACCACTTAATTGAGCGACTATACAGAGAGATCAGTGGATTGAAGGCACAGCTAGAAAACATGAAGACTGA GAGCCAGCGGGTTGTGCTGCAGCTGAAGGGCCACGTCAGCGAGCTGGAAGCAGATCTGGCCGAGCAGCAG CACCTGCGGCAGCAGGCGGCCGACGACTGTGAATTCCTGCGGGCAGAACTGGACGAGCTCAGGAGGCAGC GGGAGGACACCGAGAAGGCTCAGCGGAGCCTGTCTGAGATAGAAAGGAAAGCTCAAGCCAATGAACAGCG ATATAGCAAGCTAAAGGAGAAGTACAGCGAGCTGGTTCAGAACCACGCTGACCTGCTGCGGAAGAATGCA GAGGTGACCAAACAGGTGTCCATGGCCAGACAAGCCCAGGTAGATTTGGAACGAGAGAAAAAAGAGCTGG AGGATTCGTTGGAGCGCATCAGTGACCAGGGCCAGCGGAAGACTCAAGAACAGCTGGAAGTTCTAGAGAG CTTGAAGCAGGAACTTGCCACAAGCCAACGGGAGCTTCAGGTTCTGCAAGGCAGCCTGGAAACTTCTGCC CAGTCAGAAGCAAACTGGGCAGCCGAGTTCGCCGAGCTAGAGAAGGAGCGGGACAGCCTGGTGAGTGGCG CAGCTCATAGGGAGGAGGAATTATCTGCTCTTCGGAAAGAACTGCAGGACACTCAGCTCAAACTGGCCAG CACAGAGGAATCTATGTGCCAGCTTGCCAAAGACCAACGAAAAATGCTTCTGGTGGGGTCCAGGAAGGCT GCGGAGCAGGTGATACAAGACGCCCTGAACCAGCTTGAAGAACCTCCTCTCATCAGCTGCGCTGGGTCTG CAGATCACCTCCTCTCCACGGTCACATCCATTTCCAGCTGCATCGAGCAACTGGAGAAAAGCTGGAGCCA GTATCTGGCCTGCCCAGAAGACATCAGTGGACTTCTCCATTCCATAACCCTGCTGGCCCACTTGACCAGC GACGCCATTGCTCATGGTGCCACCACCTGCCTCAGAGCCCCACCTGAGCCTGCCGACTCACTGACCGAGG CCTGTAAGCAGTATGGCAGGGAAACCCTCGCCTACCTGGCCTCCCTGGAGGAAGAGGGAAGCCTTGAGAA TGCCGACAGCACAGCCATGAGGAACTGCCTGAGCAAGATCAAGGCCATCGGCGAGGAGCTCCTGCCCAGG GGACTGGACATCAAGCAGGAGGAGCTGGGGGACCTGGTGGACAAGGAGATGGCGGCCACTTCAGCTGCTA TTGAAACTGCCACGGCCAGAATAGAGGGTACAGCATCCCCTAAAGAGTTTTATGCCAAGAACTCTCGATG GACAGAAGGACTTATCTCAGCCTCCAAGGCTGTGGGCTGGGGAGCCACTGTCATGGTGGATGCAGCTGAT CTGGTGGTACAAGGCAGAGGGAAATTTGAGGAGCTAATGGTGTGTTCTCATGAAATTGCTGCTAGCACAG CCCAGCTTGTGGCTGCATCCAAGGTGAAAGCTGATAAGGACAGCCCCAACCTAGCCCAGCTGCAGCAGGC CTCTCGGGGAGTGAACCAGGCCACTGCCGGCGTTGTGGCCTCAACCATTTCCGGCAAATCACAGATCGAA GAGACAGACAACATGGACTTCTCAAGCATGACGCTGACACAGATCAAACGCCAAGAGATGGATTCTCAGG TTAGGGTGCTAGAGCTAGAAAATGAATTGCAGAAGGAGCGTCAAAAACTGGGAGAGCTTCGGAAAAAGCA CTACGAGCTTGCTGGTGTTGCTGAGGGCTGGGAAGAAGGAACAGAGGCATCTCCACCTACACTGCAAGAA GTGGTAACCGAAAAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC235103 representing NM_001243198
Red=Cloning site Green=Tags(s) MDRMASSMKQVPNPLPKVLSRRGVGAGLEAAERESFERTQTVSINKAINTQEVAVKEKHARTCILGTHHE KGAQTFWSVVNRLPLSSNAVLCWKFCHVFHKLLRDGHPNVLKDSLRYRNELSDMSRMWGHLSEGYGQLCS IYLKLLRTKMEYHTKNPRFPGNLQMSDRQLDEAGESDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSR SVSVTAAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFMEQFTKLKDLFYRSSNLQ YFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAEASSPDSEPVLEKDDLMDMDASQQNLFDNKFDDI FGSSFSSDPFNFNSQNGVNKDEKDHLIERLYREISGLKAQLENMKTESQRVVLQLKGHVSELEADLAEQQ HLRQQAADDCEFLRAELDELRRQREDTEKAQRSLSEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNA EVTKQVSMARQAQVDLEREKKELEDSLERISDQGQRKTQEQLEVLESLKQELATSQRELQVLQGSLETSA QSEANWAAEFAELEKERDSLVSGAAHREEELSALRKELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKA AEQVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTS DAIAHGATTCLRAPPEPADSLTEACKQYGRETLAYLASLEEEGSLENADSTAMRNCLSKIKAIGEELLPR GLDIKQEELGDLVDKEMAATSAAIETATARIEGTASPKEFYAKNSRWTEGLISASKAVGWGATVMVDAAD LVVQGRGKFEELMVCSHEIAASTAQLVAASKVKADKDSPNLAQLQQASRGVNQATAGVVASTISGKSQIE ETDNMDFSSMTLTQIKRQEMDSQVRVLELENELQKERQKLGELRKKHYELAGVAEGWEEGTEASPPTLQE VVTEKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001243198 |
ORF Size | 2958 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001243198.1, NM_001243198.2, NP_001230127.1 |
RefSeq Size | 7903 bp |
RefSeq ORF | 2961 bp |
Locus ID | 3092 |
Cytogenetics | 7q11.23 |
Protein Families | Druggable Genome |
Protein Pathways | Huntington's disease |
MW | 111.1 kDa |
Gene Summary | 'The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]' |
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