MAN2C1 (NM_001256495) Human Tagged ORF Clone

CAT#: RC235120

MAN2C1 (Myc-DDK tagged) - Homo sapiens mannosidase, alpha, class 2C, member 1 (MAN2C1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAN2C1
• Synonyms: MAN6A8; MANA; MANA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235120 representing NM_001256495
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCGCCGGCCTTGAAGCACTGGCGCACCACGCTGGAGCGGGTGGAGAAGTTCGTGTCGCCGC
  TCTACTTTACCGACTGTAACCTCCGCGGCAGGCTTTTTGGGGCCAGCTGCCCTGTGGCTGTGCTCTCCAG
  CTTCCTGACGCCGGAGAGACTTCCCTACCAGGAGGCAGTCCAGCGGGACTTCCGCCCCGCGCAGGTCGGC
  GACAGCTTCGGACCCACATGGTGGACCTGCTGGTTCCGGGTGGAGCTGACCATCCCAGAGGCATGGGTGG
  GCCAGGAAGTTCACCTTTGCTGGGAAAGTGATGGAGAAGGTCTGGTGTGGCGTGATGGAGAACCTGTCCA
  GGGTTTAACCAAAGAGGGTGAGAAGACCAGCTATGTCCTGACTGACAGGCTGGGGGAAAGAGACCCCCGA
  AGCCTCACTCTCTATGTGGAAGTAGCCTGCAATGGGCTCCTGGGGGCCGGGAAGGGAAGCATGATTGCAG
  CCCCTGACCCTGAGAAGATGTTCCAGCTGAGCCGGGCTGAGCTAGCTGTGTTCCACCGGGATGTCCACAT
  GCTCCTGGTGGATCTGGAGCTGCTGCTGGGCATAGCCAAGGGCCTCGGGAAGGACAACCAGCGCAGCTTC
  CAGGCCCTGTACACAGCCAATCAGATGGTGAACGTGTGTGACCCTGCCCAGCCCGAGACCTTCCCAGTGG
  CCCAGGCCCTGGCCTCCAGGTTCTTTGGCCAACATGGGGGTGAAAGCCAACACACCATTCATGCCACAGG
  GCACTGCCACATTGATACAGCCTGGCTTTGGCCCTTCAAAGAGACTGTGAGGAAATGTGCCCGGAGCTGG
  GTGACCGCCCTGCAGCTCATGGAGCGGAACCCTGAGTTCATCTTTGCCTGCTCCCAGGCGCAGCAGCTGG
  AATGGGTGAAGAGCCGCTACCCTGGCCTGTACTCCCGCATCCAGGAGTTTGCGTGCCGTGGGCAGTTTGT
  GCCTGTGGGGGGCACCTGGGTGGAGATGGATGGGAACCTGCCCAGTGGAGAGGCCATGGTGAGGCAGTTT
  TTGCAGGGCCAGAACTTCTTTCTGCAGGAGTTTGGGAAGATGTGCTCTGAGTTCTGGCTGCCGGACACCT
  TTGGCTACTCAGCACAGCTCCCCCAGATCATGCACGGCTGTGGCATCAGGCGCTTTCTCACCCAGAAATT
  GAGCTGGAATTTGGTGAACTCCTTCCCACACCATACATTTTTCTGGGAGGGCCTGGATGGCTCCCGTGTA
  CTGGTCCACTTCCCACCTGGCGACTCCTATGGGATGCAGGGCAGCGTGGAGGAGGTGCTGAAGACCGTGG
  CCAACAACCGGGACAAGGGGCGGGCCAACCACAGTGCCTTCCTCTTTGGCTTTGGGGATGGGGGTGGTGG
  CCCCACCCAGACCATGCTGGACCGCCTGAAGCGCCTGAGCAATACGGATGGGCTGCCCAGGGTGCAGCTA
  TCTTCTCCAAGACAGCTCTTCTCAGCACTGGAGAGTGACTCAGAGCAGCTGTGCACGTGGGTTGGGGAGC
  TCTTCTTGGAGCTGCACAATGGCACATACACCACCCATGCCCAGATCAAGAAGGGGAACCGGGAATGTGA
  GCGGATCCTGCACGACGTGGAGCTGCTCAGTAGCCTGGCCCTGGCCCGCAGTGCCCAGTTCCTATACCCA
  GCAGCCCAGCTGCAGCACCTCTGGAGGCTCCTTCTTCTGAACCAGTTCCATGATGTGGTGACTGGAAGCT
  GCATCCAGATGGTGGCAGAGGAAGCCATGTGCCATTATGAAGACATCCGTTCCCATGGCAATACACTGCT
  CAGCGCTGCAGCCGCAGCCCTGTGTGCTGGGGAGCCAGGTCCTGAGGGCCTCCTCATCGTCAACACACTG
  CCCTGGAAGCGGATCGAAGTGATGGCCCTGCCCAAACCGGGCGGGGCCCACAGCCTAGCCCTGGTGACAG
  TGCCCAGCATGGGCTATGCTCCTGTTCCTCCCCCCACCTCACTGCAGCCCCTGCTGCCCCAGCAGCCTGT
  GTTCGTAGTGCAAGAGACTGATGGCTCCGTGACTCTGGACAATGGCATCATCCGAGTGAAGCTGGACCCA
  ACTGGTCGCCTGACGTCCTTGGTCCTGGTGGCCTCTGGCAGGGAGGCCATTGCTGAGGGCGCCGTGGGGA
  ACCAGTTTGTGCTATTTGATGATGTCCCCTTGTACTGGGATGCATGGGACGTCATGGACTACCACCTGGA
  GACACGGAAGCCTGTGCTGGGCCAGGCAGGGACCCTGGCAGTGGGCACCGAGGGCGGCCTGCGGGGCAGC
  GCCTGGTTCTTGCTACAGATCAGCCCCAACAGTCGGCTTAGCCAGGAGGTTGTGCTGGACGTTGGCTGCC
  CCTATGTCCGCTTCCACACCGAGGTACACTGGCATGAGGCCCACAAGTTCCTGAAGGTGGAGTTCCCTGC
  TCGCGTGCGGAGTTCCCAGGCCACCTATGAGATCCAGTTTGGGCACCTGCAGCGACCTACCCACTACAAT
  ACCTCTTGGGACTGGGCTCGATTTGAGGTGTGGGCCCATCGCTGGATGGATCTGTCAGAACACGGCTTTG
  GGCTGGCCCTGCTCAACGACTGCAAGTATGGCGCGTCAGTGCGAGGCAGCATCCTCAGCCTCTCGCTCTT
  GCGGGCGCCTAAAGCCCCGGACGCTACTGCTGACACGGGGCGCCACGAGTTCACCTATGCACTGATGCCG
  CACAAGGCCCCCAGCCCAGCGCCCGCCACCTCCTGGAGTGCGTTTTCCGTGTCTTCACCCGCGGTCGTAT
  TGGAGACCGTCAAGCAGGCGGAGAGCAGCCCCCAGCGCCGCTCGCTGGTCCTGAGGCTGTATGAGGCCCA
  CGGCAGCCACGTGGACTGCTGGCTGCACTTGTCGCTGCCGGTTCAGGAGGCCATCCTCTGCGATCTCTTG
  GAGCGACCAGACCCTGCTGGCCACTTGACCCTTCGGGACAACCGCCTGAAGCTCACCTTTTCTCCCTTCC
  AAGTGCTGTCCCTGTTGCTCGTGCTTCAGCCTCCGCCACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235120 representing NM_001256495
  Red=Cloning site Green=Tags(s)
  
  MAAAPALKHWRTTLERVEKFVSPLYFTDCNLRGRLFGASCPVAVLSSFLTPERLPYQEAVQRDFRPAQVG
  DSFGPTWWTCWFRVELTIPEAWVGQEVHLCWESDGEGLVWRDGEPVQGLTKEGEKTSYVLTDRLGERDPR
  SLTLYVEVACNGLLGAGKGSMIAAPDPEKMFQLSRAELAVFHRDVHMLLVDLELLLGIAKGLGKDNQRSF
  QALYTANQMVNVCDPAQPETFPVAQALASRFFGQHGGESQHTIHATGHCHIDTAWLWPFKETVRKCARSW
  VTALQLMERNPEFIFACSQAQQLEWVKSRYPGLYSRIQEFACRGQFVPVGGTWVEMDGNLPSGEAMVRQF
  LQGQNFFLQEFGKMCSEFWLPDTFGYSAQLPQIMHGCGIRRFLTQKLSWNLVNSFPHHTFFWEGLDGSRV
  LVHFPPGDSYGMQGSVEEVLKTVANNRDKGRANHSAFLFGFGDGGGGPTQTMLDRLKRLSNTDGLPRVQL
  SSPRQLFSALESDSEQLCTWVGELFLELHNGTYTTHAQIKKGNRECERILHDVELLSSLALARSAQFLYP
  AAQLQHLWRLLLLNQFHDVVTGSCIQMVAEEAMCHYEDIRSHGNTLLSAAAAALCAGEPGPEGLLIVNTL
  PWKRIEVMALPKPGGAHSLALVTVPSMGYAPVPPPTSLQPLLPQQPVFVVQETDGSVTLDNGIIRVKLDP
  TGRLTSLVLVASGREAIAEGAVGNQFVLFDDVPLYWDAWDVMDYHLETRKPVLGQAGTLAVGTEGGLRGS
  AWFLLQISPNSRLSQEVVLDVGCPYVRFHTEVHWHEAHKFLKVEFPARVRSSQATYEIQFGHLQRPTHYN
  TSWDWARFEVWAHRWMDLSEHGFGLALLNDCKYGASVRGSILSLSLLRAPKAPDATADTGRHEFTYALMP
  HKAPSPAPATSWSAFSVSSPAVVLETVKQAESSPQRRSLVLRLYEAHGSHVDCWLHLSLPVQEAILCDLL
  ERPDPAGHLTLRDNRLKLTFSPFQVLSLLLVLQPPPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256495
• ORF Size: 3051 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256495.1, NP_001243424.1
• RefSeq Size: 3222 bp
• RefSeq ORF: 3054 bp
• Locus ID: 4123
• Cytogenetics: 15q24.2
• Protein Families: Druggable Genome
• Protein Pathways: Other glycan degradation
• MW: 113.9 kDa
• Gene Summary: ''