ERCC6 (NM_001277058) Human Tagged ORF Clone

CAT#: RC235145

ERCC6-PGBD3 (Myc-DDK tagged) - Homo sapiens ERCC6-PGBD3 readthrough (ERCC6-PGBD3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ERCC6
• Synonyms: ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3; POF11; RAD26; UVSS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235145 representing NM_001277058
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAATGAGGGAATCCCCCACTCAAGTCAAACTCAGGAGCAAGACTGTTTACAGAGTCAACCTGTCA
  GTAATAATGAAGAAATGGCAATCAAGCAAGAAAGTGGTGGTGATGGGGAGGTGGAGGAGTACCTCTCCTT
  TCGTTCTGTGGGTGACGGGCTGTCCACCTCTGCTGTGGGGTGCGCATCAGCAGCTCCGAGGAGAGGGCCA
  GCCCTGCTGCACATCGACCGACATCAGATCCAGGCAGTAGAGCCTAGCGCCCAGGCCCTTGAGCTGCAGG
  GTTTGGGTGTGGACGTCTATGACCAGGACGTGCTGGAACAGGGAGTGCTTCAGCAGGTGGACAATGCCAT
  CCATGAGGCCAGCCGTGCCTCCCAGCTCGTTGACGTGGAGAAGGAGTATCGGTCGGTCCTGGATGACCTC
  ACGTCATGTACGACATCCCTAAGGCAAATCAATAAAATTATTGAACAGCTTAGCCCTCAAGCTGCCACCA
  GCAGAGACATCAACAGGAAACTAGATTCTGTAAAACGACAGAAGTATAATAAGGAACAACAGCTAAAAAA
  GATCACTGCAAAACAAAAGCATCTCCAGGCCATCCTTGGAGGAGCAGAGGTGAAAATTGAACTAGATCAC
  GCCAGTCTGGAGGAGGATGCAGAGCCGGGGCCATCCAGTCTTGGCAGCATGCTCATGCCTGTCCAGGAGA
  CTGCCTGGGAAGAGCTCATCCGCACTGGCCAGATGACACCTTTTGGTACCCAGATCCCTCAGAAACAGGA
  GAAAAAGCCCAGAAAAATCATGCTTAATGAAGCATCAGGCTTCGAAAAGTATTTGGCAGATCAAGCAAAA
  CTGTCTTTTGAAAGGAAGAAGCAAGGTTGTAATAAAAGAGCAGCTAGAAAAGCTCCAGCCCCAGTCACGC
  CTCCAGCCCCAGTGCAAAATAAAAACAAACCAAACAAGAAAGCCAGAGTTCTGTCCAAAAAAGAGGAGCG
  TTTGAAAAAGCACATCAAGAAACTCCAGAAGAGGGCTTTGCAGTTCCAGGGGAAAGTGGGATTGCCAAAG
  GCAAGGAGACCTTGGGAGTCAGACATGAGGCCAGAGGCAGAGGGAGACTCTGAGGGTGAAGAGTCTGAGT
  ATTTCCCCACAGAGGAGGAGGAAGAGGAGGAAGATGACGAGGTGGAGGGGGCAGAGGCGGACCTGTCTGG
  AGATGGTACTGACTATGAGCTGAAGCCTCTGCCCAAGGGCGGGAAACGGCAGAAGAAAGTGCCAGTGCAG
  GAGATTGATGATGACTTTTTCCCAAGTTCTGGGGAAGAAGCTGAAGCTGCTTCTGTAGGAGAAGGAGGAG
  GAGGAGGTCGGAAAGTGGGAAGATACCGAGATGATGGAGATGAAGATTATTATAAGCAGCGGTTAAGTCC
  CAAGATGCCTCGAACACTAAGTTTACATGAAATAACTGACCTTTTAGAGACAGATGACAGCATAGAAGCA
  AGTGCTATAGTGATACAACCACCTGAAAATGCTACAGCACCTGTTTCTGATGAGGAATCAGGAGATGAAG
  AAGGTGGAACAATAAATAATCTGCCAGGTTCTTTGTTGCACACAGCTGCGTATCTTATTCAAGATGGCTC
  TGATGCTGAGTCTGACTCAGATGATCCCTCATACGCACCTAAAGATGACTCTCCTGATGAAGTTCCATCT
  ACGTTTACTGTGCAGCAACCTCCACCATCAAGGAGGAGGAAAATGACAAAAATTCTTTGCAAATGGAAAA
  AAGCCGACCTAACTGTACAACCCGTAGCAGGTAGAGTTACAGCACCACCAAACGATTTCTTCACCGTAAT
  GAGAACTCCCACAGAAATTCTTGAACTTTTTCTTGATGACGAGGTCATTGAACTCATTGTCAAGTACTCC
  AACTTATATGCTTGCAGTAAAGGTGTACATCTTGGCTTGACTAGCTCTGAATTCAAATGTTTTCTGGGAA
  TTATTTTTCTGAGTGGTTATGTCTCAGTTCCTAGAAGGCGTATGTTTTGGGAACAAAGAACAGATGTGCA
  TAATGTACTGGTTAGTGCTGCCATGAGACGTGACCGGTTTGAAACTATATTTTCTAATTTGCATGTTGCT
  GACAATGCAAATTTGGATCCAGTGGACAAATTTTCCAAATTGCGACCTCTCATAAGCAAACTTAATGAGA
  GATGCATGAAATTTGTTCCAAATGAAACATATTTCAGCTTTGATGAATTCATGGTTCCTTATTTTGGTCG
  TCACGGGTGCAAACAATTTATTCGGGGAAAGCCCATTCGGTTTGGCTATAAGTTTTGGTGTGGTGCCACC
  TGTCTGGGCTACATTTGCTGGTTTCAGCCGTATCAGGGTAAAAACCCAAATACTAAACATGAGGAATATG
  GTGTCGGTGCGTCACTTGTCCTTCAGTTTAGTGAGGCACTTACAGAGGCACACCCTGGACAATACCATTT
  TGTATTCAATAACTTTTTCACCAGTATTGCACTTCTTGATAAGCTCAGTTCAATGGGACATCAGGCAACA
  GGTACAGTGAGAAAGGATCACATTGACAGAGTTCCACTGGAATCAGATGTAGCTTTAAAGAAAAAAGAAA
  GAGGCACATTTGATTATCGAATTGATGGCAAAGGCAATATTGTCTGCAGATGGAATGATAACAGTGTTGT
  CACTGTTGCCTCATCTGGTGCTGGTATCCATCCCCTGTGTCTTGTCAGTCGTTACTCCCAGAAACTGAAA
  AAGAAGATACAAGTTCAGCAGCCAAACATGATCAAAGTGTATAACCAGTTCATGGGAGGCGTAGACAGAG
  CTGATGAAAACATTGATAAGTATCGGGCATCAATCCGTGGAAAGAAATGGTATTCAAGCCCTCTTTTGTT
  CTGTTTCGAACTGGTCTTACAAAATGCTTGGCAATTGCATAAAACATATGATGAGAAACCAGTGGATTTT
  CTGGAGTTTCGTCGACGTGTGGTATGCCATTATCTGGAGACCCATGGTCATCCTCCAGAACCTGGCCAAA
  AAGGAAGACCTCAGAAGCGTAACATTGACTCACGTTATGATGGCATAAATCATGTGATAGTCAAACAGGG
  AAAGCAAACGCGATGCGCTGAATGTCATAAGAACACAACTTTTCGATGTGAAAAATGTGATGTTGCCTTA
  CATGTGAAGTGTTCCGTTGAATATCACACTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235145 representing NM_001277058
  Red=Cloning site Green=Tags(s)
  
  MPNEGIPHSSQTQEQDCLQSQPVSNNEEMAIKQESGGDGEVEEYLSFRSVGDGLSTSAVGCASAAPRRGP
  ALLHIDRHQIQAVEPSAQALELQGLGVDVYDQDVLEQGVLQQVDNAIHEASRASQLVDVEKEYRSVLDDL
  TSCTTSLRQINKIIEQLSPQAATSRDINRKLDSVKRQKYNKEQQLKKITAKQKHLQAILGGAEVKIELDH
  ASLEEDAEPGPSSLGSMLMPVQETAWEELIRTGQMTPFGTQIPQKQEKKPRKIMLNEASGFEKYLADQAK
  LSFERKKQGCNKRAARKAPAPVTPPAPVQNKNKPNKKARVLSKKEERLKKHIKKLQKRALQFQGKVGLPK
  ARRPWESDMRPEAEGDSEGEESEYFPTEEEEEEEDDEVEGAEADLSGDGTDYELKPLPKGGKRQKKVPVQ
  EIDDDFFPSSGEEAEAASVGEGGGGGRKVGRYRDDGDEDYYKQRLSPKMPRTLSLHEITDLLETDDSIEA
  SAIVIQPPENATAPVSDEESGDEEGGTINNLPGSLLHTAAYLIQDGSDAESDSDDPSYAPKDDSPDEVPS
  TFTVQQPPPSRRRKMTKILCKWKKADLTVQPVAGRVTAPPNDFFTVMRTPTEILELFLDDEVIELIVKYS
  NLYACSKGVHLGLTSSEFKCFLGIIFLSGYVSVPRRRMFWEQRTDVHNVLVSAAMRRDRFETIFSNLHVA
  DNANLDPVDKFSKLRPLISKLNERCMKFVPNETYFSFDEFMVPYFGRHGCKQFIRGKPIRFGYKFWCGAT
  CLGYICWFQPYQGKNPNTKHEEYGVGASLVLQFSEALTEAHPGQYHFVFNNFFTSIALLDKLSSMGHQAT
  GTVRKDHIDRVPLESDVALKKKERGTFDYRIDGKGNIVCRWNDNSVVTVASSGAGIHPLCLVSRYSQKLK
  KKIQVQQPNMIKVYNQFMGGVDRADENIDKYRASIRGKKWYSSPLLFCFELVLQNAWQLHKTYDEKPVDF
  LEFRRRVVCHYLETHGHPPEPGQKGRPQKRNIDSRYDGINHVIVKQGKQTRCAECHKNTTFRCEKCDVAL
  HVKCSVEYHTE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277058
• ORF Size: 3183 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001277058.1, NP_001263987.1
• RefSeq Size: 3590
• RefSeq ORF: 3186
• Locus ID: 101243544
• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair
• MW: 119.9 kDa
• Gene Summary: This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The encoded protein has ATP-stimulated ATPase activity, interacts with several transcription and excision repair proteins, and may promote complex formation at DNA repair sites. Mutations in this gene are associated with Cockayne syndrome type B and cerebrooculofacioskeletal syndrome 1. Alternative splicing occurs between a splice site from exon 5 of this gene to the 3' splice site upstream of the open reading frame (ORF) of the adjacent gene, piggyback-derived-3 (GeneID:267004), which activates the alternative polyadenylation site downstream of the piggyback-derived-3 ORF. The resulting transcripts encode a fusion protein that shares sequence with the product of each individual gene. [provided by RefSeq, Mar 2016]