UTY (NM_001258250) Human Tagged ORF Clone

CAT#: RC235215

UTY (Myc-DDK tagged) - Homo sapiens ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (UTY), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UTY
• Synonyms: KDM6AL; KDM6C; UTY1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235215 representing NM_001258250
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCCTGCGCAGTGTCGCTCACTACCGCCGCTGTTGCCTTCGGTGATGAGGCAAAGAAAATGGCGG
  AAGGAAAAGCGAGCCGCGAGAGTGAAGAGGAGTCTGTTAGCCTGACAGTCGAGGAAAGGGAGGCGCTTGG
  TGGCATGGACAGCCGTCTCTTCGGGTTCGTGAGGCTTCATGAAGATGGCGCCAGAACGAAGACCCTACTA
  GGCAAGGCTGTTCGCTGCTACGAATCTTTAATCTTAAAAGCTGAAGGAAAAGTGGAGTCTGACTTCTTTT
  GCCAATTAGGTCACTTCAACCTCTTGTTGGAAGATTATTCAAAAGCATTATCTGCATATCAGAGATATTA
  CAGTTTACAGGCTGACTACTGGAAGAATGCTGCGTTTTTATATGGCCTTGGTTTGGTCTACTTCTACTAC
  AATGCATTTCATTGGGCAATTAAAGCATTTCAAGATGTCCTTTATGTTGACCCCAGCTTTTGTCGAGCCA
  AGGAAATTCATTTACGACTTGGGCTCATGTTCAAAGTGAACACAGACTACAAGTCTAGTTTAAAGCATTT
  TCAGTTAGCCTTGATTGACTGTAATCCATGTACTTTGTCCAATGCTGAAATTCAATTTCATATTGCCCAT
  TTGTATGAAACCCAGAGGAAGTATCATTCTGCAAAGGAGGCATATGAACAACTTTTGCAGACAGAAAACC
  TTCCTGCACAAGTAAAAGCAACTGTATTGCAACAGTTAGGTTGGATGCATCATAATATGGATCTAGTAGG
  AGACAAAGCCACAAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCTTTGGAGGCAGATCCTAATTCT
  GGCCAATCGTGGTATTTTCTTGGAAGGCAATCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTT
  CAATAGGTGTGTTGTATCAGCAGCAAAATCAGCCTATGGATGCTTTACAGGCATATATTTGTGCTGTACA
  ATTGGACCATGGGCATGCCGCAGCCTGGATGGACCTAGGTACTCTCTATGAATCCTGCAATCAACCTCAA
  GATGCCATTAAATGCTACCTAAATGCAGCTAGAAGCAAACGTTGTAGTAATACCTCTACGCTTGCTGCAA
  GAATTAAATTTCTACAGAATGGTTCTGATAACTGGAATGGTGGCCAGAGTCTTTCACATCATCCAGTACA
  GCAAGTTTATTCGTTGTGTTTGACACCACAGAAATTACAGCACTTGGAACAACTGCGAGCAAATAGAGAT
  AATTTAAATCCAGCACAGAAGCATCAGCTGGAACAGTTAGAAAGTCAGTTTGTCTTAATGCAGCAAATGA
  GACACAAAGAAGTTGCTCAGGGGCTTCATAAAAGTCAGAGTTCATGTTTGTCAGGACCTAATGAAGAACA
  ACCTCTGTTTTCCACTGGGTCAGCCCAGTATCACCAGGCAACTAGCACTGGTATTAAGAAGGCGAATGAA
  CATCTCACTCTGCCTAGTAATTCAGTACCACAGGGGGATGCTGACAGTCACCTCTCCTGTCATACTGCTA
  CCTCAGGTGGACAACAAGGCATTATGTTTACCAAAGAGAGCAAGCCTTCAAAAAATAGATCCTTGGTGCC
  TGAAACAAGCAGGCATACTGGAGACACATCTAATGGCTGTGCTGATGTCAAGGGACTTTCTAATCATGTT
  CATCAGTTGATAGCAGATGCTGTTTCCAGTCCTAACCATGGAGATTCACCAAATTTATTAATTGCAGACA
  ATCCTCAGCTCTCTGCTTTGTTGATTGGAAAAGCCAATGGCAATGTGGGTACTGGAACCTGTGACAAAGT
  GAATAATATTCACCCAGCTGTTCATACAAAGACTGATCATTCTGTTGCCTCTTCACCCTCTTCAGCCATT
  TCCACAGCAACACCTTCTCCTAAATCCACTGAGCAGAGAAGCATAAACAGTGTTACCAGCCTTAACAGTC
  CTCACAGTGGATTACACACAGTCAATGGAGAGGGGCTGGGGAAGTCACAGAGCTCTACAAAAGTAGACCT
  GCCTTTAGCTAGCCACAGATCTACTTCTCAGATCTTACCATCAATGTCAGTGTCTATATGCCCCAGTTCA
  ACAGAAGTTCTGAAAGCATGCAGGAATCCAGGTAAAAATGGCTTGTCTAATAGCTGCATTTTGTTAGATA
  AATGTCCACCTCCAAGACCACCAACTTCACCATACCCACCCTTGCCAAAGGACAAGTTGAATCCACCCAC
  ACCTAGTATTTACTTGGAAAATAAACGTGATGCTTTCTTTCCTCCATTACATCAATTTTGTACAAATCCA
  AAAAACCCTGTTACAGTAATACGTGGCCTTGCTGGAGCTCTTAAATTAGATCTTGGACTTTTCTCTACCA
  AAACTTTGGTAGAAGCTAACAATGAACATATGGTAGAAGTGAGGACACAGTTGCTGCAACCAGCAGATGA
  AAACTGGGATCCCACTGGAACAAAGAAAATCTGGCGTTGTGAAAGCAATAGATCTCATACTACAATTGCC
  AAATACGCACAATACCAGGCTTCCTCCTTCCAGGAATCATTGAGAGAAGAAAATGAGAAAAGAACACAAC
  ACAAAGATCATTCAGATAACGAATCCACATCTTCAGAGAATTCTGGAAGGAGAAGGAAAGGACCTTTTAA
  AACCATAAAATTTGGGACCAACATTGACCTCTCTGATAACAAAAAGTGGAAGTTGCAGTTACATGAACTG
  ACTAAACTTCCTGCTTTTGCGCGTGTGGTGTCAGCAGGAAATCTTCTAACCCATGTTGGGCATACCATTC
  TGGGCATGAATACAGTACAACTGTATATGAAAGTTCCAGGGAGTCGGACACCAGGTCACCAAGAAAATAA
  CAACTTCTGCTCTGTTAACATAAATATTGGTCCAGGAGATTGTGAATGGTTTGTTGTACCTGAAGATTAT
  TGGGGTGTTCTGAATGACTTCTGTGAAAAAAATAATTTGAATTTTTTAATGAGTTCTTGGTGGCCCAACC
  TTGAAGATCTTTATGAAGCAAATGTCCCTGTGTATAGATTTATTCAGCGACCTGGAGATTTGGTCTGGAT
  AAATGCAGGCACTGTGCATTGGGTTCAAGCTGTTGGCTGGTGCAATAACATTGCCTGGAATGTTGGTCCA
  CTTACAGCCTGCCAGTATAAATTGGCAGTGGAACGGTATGAATGGAACAAATTGAAAAGTGTGAAGTCAC
  CAGTACCCATGGTGCATCTTTCCTGGAATATGGCACGAAATATCAAAGTCTCAGATCCAAAGCTTTTTGA
  AATGATTAAGTATTGTCTTTTGAAAATTCTGAAGCAATATCAGACATTGAGAGAAGCTCTTGTTGCAGCA
  GGAAAAGAGGTTATATGGCATGGGCGGACAAATGATGAACCAGCTCATTACTGTAGCATTTGTGAGGTGG
  AGGTTTTTAATCTGCTTTTTGTCACTAATGAAAGCAATACTCAAAAAACCTACATAGTACATTGCCATGA
  TTGTGCACGAAAAACAAGCAAAAGTTTGGAAAATTTTGTGGTGCTCGAACAGTACAAAATGGAGGACCTA
  ATCCAAGTTTATGATCAATTTACACTAGCTCTTTCATTATCATCCTCATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235215 representing NM_001258250
  Red=Cloning site Green=Tags(s)
  
  MKSCAVSLTTAAVAFGDEAKKMAEGKASRESEEESVSLTVEEREALGGMDSRLFGFVRLHEDGARTKTLL
  GKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYSKALSAYQRYYSLQADYWKNAAFLYGLGLVYFYY
  NAFHWAIKAFQDVLYVDPSFCRAKEIHLRLGLMFKVNTDYKSSLKHFQLALIDCNPCTLSNAEIQFHIAH
  LYETQRKYHSAKEAYEQLLQTENLPAQVKATVLQQLGWMHHNMDLVGDKATKESYAIQYLQKSLEADPNS
  GQSWYFLGRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQ
  DAIKCYLNAARSKRCSNTSTLAARIKFLQNGSDNWNGGQSLSHHPVQQVYSLCLTPQKLQHLEQLRANRD
  NLNPAQKHQLEQLESQFVLMQQMRHKEVAQGLHKSQSSCLSGPNEEQPLFSTGSAQYHQATSTGIKKANE
  HLTLPSNSVPQGDADSHLSCHTATSGGQQGIMFTKESKPSKNRSLVPETSRHTGDTSNGCADVKGLSNHV
  HQLIADAVSSPNHGDSPNLLIADNPQLSALLIGKANGNVGTGTCDKVNNIHPAVHTKTDHSVASSPSSAI
  STATPSPKSTEQRSINSVTSLNSPHSGLHTVNGEGLGKSQSSTKVDLPLASHRSTSQILPSMSVSICPSS
  TEVLKACRNPGKNGLSNSCILLDKCPPPRPPTSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNP
  KNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWRCESNRSHTTIA
  KYAQYQASSFQESLREENEKRTQHKDHSDNESTSSENSGRRRKGPFKTIKFGTNIDLSDNKKWKLQLHEL
  TKLPAFARVVSAGNLLTHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEDY
  WGVLNDFCEKNNLNFLMSSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGP
  LTACQYKLAVERYEWNKLKSVKSPVPMVHLSWNMARNIKVSDPKLFEMIKYCLLKILKQYQTLREALVAA
  GKEVIWHGRTNDEPAHYCSICEVEVFNLLFVTNESNTQKTYIVHCHDCARKTSKSLENFVVLEQYKMEDL
  IQVYDQFTLALSLSSSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258250
• ORF Size: 3621 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258250.1, NP_001245179.1
• RefSeq Size: 6106 bp
• RefSeq ORF: 3624 bp
• Locus ID: 7404
• Cytogenetics: Yq11.221
• Protein Families: Transmembrane
• MW: 135.3 kDa
• Gene Summary: 'This gene encodes a protein containing tetratricopeptide repeats which are thought to be involved in protein-protein interactions. The encoded protein is also a minor histocompatibility antigen which may induce graft rejection of male stem cell grafts. A large number of alternatively spliced transcripts have been observed for this gene, but the full length nature of some of these variants has not been determined. [provided by RefSeq, Apr 2012]'