MAP4K4 (NM_001242559) Human Tagged ORF Clone

CAT#: RC235235

MAP4K4 (Myc-DDK tagged) - Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAP4K4
• Synonyms: FLH21957; HEL-S-31; HGK; MEKKK4; NIK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235235 representing NM_001242559
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCTGCTGGGA
  TTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAAC
  GGGTCAGTTGGCAGCCATCAAAGTTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATA
  AATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCC
  CTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGT
  GAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGA
  CTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGA
  ATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATAC
  GTTCATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTAT
  GATTACAGAAGTGATCTTTGGTCTTGTGGCATTACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCT
  GTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGTCAAAAAA
  ATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACA
  GAGCAGCTTTTGAAACATCCTTTTATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGG
  ATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTATGAGTACAGTGGGAGTGA
  GGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCT
  ACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAAC
  AGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAA
  GCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGA
  AGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAA
  GGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTA
  TATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAG
  GCCATGTTACTGGAGTGCCGATGGCGGGAGATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGT
  TGCAACAAGAACAAGCATATCTCCTGTCTCTACAGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCA
  GCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTAC
  GAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCC
  AGTCTAAGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAA
  CTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGCGGAGCCACAGGTTCCTGTGAGAACAACATCTCGC
  TCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGA
  GAAACTCCACCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAG
  TGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCC
  GGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAG
  TGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAA
  AGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAG
  TCGGGGACGACGGATGAGGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAG
  AGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGT
  CCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGACT
  CAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGACCCCAGAT
  TACTACAGATTTCTCCATCTAGCGGAACAACAGTGACATCTGTGGTGGGATTTTCCTGTGATGGGATGAG
  ACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGG
  CCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCT
  TATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGGGAA
  GGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTTGAATGTCTTGGTG
  ACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTGGTTAAGAAATAAAATACTTCACA
  ATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACCGTAGGGGATTTGGAAGGATGTGTACATTATAA
  AGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGG
  GCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGG
  TGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGCTGT
  TGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAGCATCAAACCCCAT
  GCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTGCTATGAAGATGAGGGGGTTTATG
  TAAACACATATGGAAGGATCACCAAGGATGTAGTTCTACAGTGGGGAGAGATGCCTACATCAGTAGCATA
  TATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCAC
  TTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGT
  TCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCTTCT
  GAGCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235235 representing NM_001242559
  Red=Cloning site Green=Tags(s)
  
  MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEI
  NMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRG
  LAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATY
  DYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPST
  EQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGES
  TLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREAR
  RQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQ
  AMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHY
  EPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSR
  SPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGS
  GERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEE
  SGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQT
  QSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTR
  PQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLV
  TISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAW
  APKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPH
  AIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGH
  LDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242559
• ORF Size: 3717 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001242559.1, NP_001229488.1
• RefSeq Size: 7183
• RefSeq ORF: 3720
• Locus ID: 9448
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• MW: 142.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]