TRPM1 (NM_001252024) Human Tagged ORF Clone

CAT#: RC235323

TRPM1 (Myc-DDK tagged) - Homo sapiens transient receptor potential cation channel, subfamily M, member 1 (TRPM1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPM1
• Synonyms: CSNB1C; LTRPC1; MLSN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235323 representing NM_001252024
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCAGAAATCTTGGATAGAGAAAACCTTTTGCAAACGGGAATGTATCTTTGTAATTCCTAGCATGA
  AAGACTCTAACAGGTGTTGCTGTGGCCAGTTCACCAACCAGCATATCCCCCCTCTGCCAAGTGCAACACC
  CAGCAAAAATGAAGAGGAAAGCAAACAGGTGGAGACTCAGCCTGAGAAATGGTCTGTTGCCAAGCACACC
  CAGAGCTACCCAACAGATTCCTATGGAGTTCTTGAATTCCAGGGTGGCGGATATTCCAATAAAGCCATGT
  ATATCCGTGTATCCTATGACACCAAGCCAGACTCACTGCTCCATCTCATGGTGAAAGATTGGCAGCTGGA
  ACTCCCCAAGCTCTTAATATCTGTGCATGGAGGCCTCCAGAACTTTGAGATGCAGCCCAAGCTGAAACAA
  GTCTTTGGGAAAGGCCTGATCAAGGCTGCTATGACCACCGGGGCCTGGATCTTCACCGGGGGTGTCAGCA
  CAGGTGTTATCAGCCACGTAGGGGATGCCTTGAAAGACCACTCCTCCAAGTCCAGAGGCCGGGTTTGTGC
  TATAGGAATTGCTCCATGGGGCATCGTGGAGAATAAGGAAGACCTGGTTGGAAAGGATGTAACAAGAGTG
  TACCAGACCATGTCCAACCCTCTAAGTAAGCTCTCTGTGCTCAACAACTCCCACACCCACTTCATCCTGG
  CTGACAATGGCACCCTGGGCAAGTATGGCGCCGAGGTGAAGCTGCGAAGGCTGCTGGAAAAGCACATCTC
  CCTGCAGAAGATCAACACAAGACTGGGGCAGGGCGTGCCCCTCGTGGGTCTCGTGGTGGAGGGGGGCCCT
  AACGTGGTGTCCATCGTCTTGGAATACCTGCAAGAAGAGCCTCCCATCCCTGTGGTGATTTGTGATGGCA
  GCGGACGTGCCTCGGACATCCTGTCCTTTGCGCACAAGTACTGTGAAGAAGGCGGAATAATAAATGAGTC
  CCTCAGGGAGCAGCTTCTAGTTACCATTCAGAAAACATTTAATTATAATAAGGCACAATCACATCAGCTG
  TTTGCAATTATAATGGAGTGCATGAAGAAGAAAGAACTCGTCACTGTGTTCAGAATGGGTTCTGAGGGCC
  AGCAGGACATCGAGATGGCAATTTTAACTGCCCTGCTGAAAGGAACAAACGTATCTGCTCCAGATCAGCT
  GAGCTTGGCACTGGCTTGGAACCGCGTGGACATAGCACGAAGCCAGATCTTTGTCTTTGGGCCCCACTGG
  CCGCCCCTGGGAAGCCTGGCACCCCCGACGGACAGCAAAGCCACGGAGAAGGAGAAGAAGCCACCCATGG
  CCACCACCAAGGGAGGAAGAGGAAAAGGGAAAGGCAAGAAGAAAGGGAAAGTGAAAGAGGAAGTGGAGGA
  AGAAACTGACCCCCGGAAGATAGAGCTGCTGAACTGGGTGAATGCTTTGGAGCAAGCGATGCTAGATGCT
  TTAGTCTTAGATCGTGTCGACTTTGTGAAGCTCCTGATTGAAAACGGAGTGAACATGCAACACTTTCTGA
  CCATTCCGAGGCTGGAGGAGCTTTATAACACAAGACTGGGTCCACCAAACACACTTCATCTGCTGGTGAG
  GGATGTGAAAAAGAGCAACCTTCCGCCTGATTACCACATCAGCCTCATAGACATCGGGCTCGTGCTGGAG
  TACCTCATGGGAGGAGCCTACCGCTGCAACTACACTCGGAAAAACTTTCGGACCCTTTACAACAACTTGT
  TTGGACCAAAGAGGCCTAAAGCTCTTAAACTTCTGGGAATGGAAGATGATGAGCCTCCAGCTAAAGGGAA
  GAAAAAGAAAAAGAAGAAAAAGGAGGAAGAGATCGACATTGATGTGGACGACCCTGCCGTGAGTCGGTTC
  CAGTATCCCTTCCACGAGCTGATGGTGTGGGCAGTGCTGATGAAACGCCAGAAAATGGCAGTGTTCCTCT
  GGCAGCGAGGGGAAGAGAGCATGGCCAAGGCCCTGGTGGCCTGCAAGCTCTACAAGGCCATGGCCCACGA
  GTCCTCCGAGAGTGATCTGGTGGATGACATCTCCCAGGACTTGGATAACAATTCCAAAGACTTCGGCCAG
  CTTGCTTTGGAGTTATTAGACCAGTCCTATAAGCATGACGAGCAGATCGCTATGAAACTCCTGACCTACG
  AGCTGAAAAACTGGAGCAACTCGACCTGCCTCAAACTGGCCGTGGCAGCCAAACACCGGGACTTCATTGC
  TCACACCTGCAGCCAGATGCTGCTGACCGATATGTGGATGGGAAGACTGCGGATGCGGAAGAACCCCGGC
  CTGAAGGTTATCATGGGGATTCTTCTACCCCCCACCATCTTGTTTTTGGAATTTCGCACATATGATGATT
  TCTCGTATCAAACATCCAAGGAAAATGAGGATGGCAAAGAAAAAGAAGAGGAAAATACGGATGCAAATGC
  AGATGCTGGCTCAAGAAAGGGGGATGAGGAGAACGAGCACAAAAAACAGAGAAGTATTCCCATCGGAACA
  AAGATCTGTGAATTCTATAACGCGCCCATTGTCAAGTTCTGGTTTTACACAATATCATACTTGGGCTACC
  TGCTGCTGTTTAACTACGTCATCCTGGTGCGGATGGATGGCTGGCCGTCCCTCCAGGAGTGGATCGTCAT
  CTCCTACATCGTGAGCCTGGCGTTAGAGAAGATACGAGAGATCCTCATGTCAGAACCAGGCAAACTCAGC
  CAGAAAATCAAAGTTTGGCTTCAGGAGTACTGGAACATCACAGATCTCGTGGCCATTTCCACATTCATGA
  TTGGAGCAATTCTTCGCCTACAGAACCAGCCCTACATGGGCTATGGCCGGGTGATCTACTGTGTGGATAT
  CATCTTCTGGTACATCCGTGTCCTGGACATCTTTGGTGTCAACAAGTATCTGGGGCCATACGTGATGATG
  ATTGGAAAGATGATGATCGACATGCTGTACTTTGTGGTCATCATGCTGGTCGTGCTCATGAGTTTCGGAG
  TAGCCCGTCAAGCCATTCTGCATCCAGAGGAGAAGCCCTCTTGGAAACTGGCCCGAAACATCTTCTACAT
  GCCCTACTGGATGATCTATGGAGAGGTGTTTGCAGACCAGATAGACCTCTACGCCATGGAAATTAATCCT
  CCTTGTGGTGAGAACCTATATGATGAGGAGGGCAAGCGGCTTCCTCCCTGTATCCCCGGCGCCTGGCTCA
  CTCCAGCACTCATGGCGTGCTATCTACTGGTCGCCAACATCCTGCTGGTGAACCTGCTGATTGCTGTGTT
  CAACAATACCTTCTTTGAAGTAAAATCAATATCCAACCAGGTGTGGAAGTTCCAGCGATATCAGCTGATT
  ATGACATTTCATGACAGGCCAGTCCTGCCCCCACCGATGATCATTTTAAGCCACATCTACATCATCATTA
  TGCGTCTCAGCGGCCGCTGCAGGAAAAAGAGAGAAGGGGACCAAGAGGAACGGGATCGTGGATTGAAGCT
  CTTCCTTAGCGACGAGGAGCTAAAGAGGCTGCATGAGTTCGAGGAGCAGTGCGTGCAGGAGCACTTCCGG
  GAGAAGGAGGATGAGCAGCAGTCGTCCAGCGACGAGCGCATCCGGGTCACTTCTGAAAGAGTTGAAAATA
  TGTCAATGAGGTTGGAAGAAATCAATGAAAGAGAAACTTTTATGAAAACTTCCCTGCAGACTGTTGACCT
  TCGACTTGCTCAGCTAGAAGAATTATCTAACAGAATGGTGAATGCTCTTGAAAATCTTGCGGGAATCGAC
  AGGTCTGACCTGATCCAGGCACGGTCCCGGGCTTCTTCTGAATGTGAGGCAACGTATCTTCTCCGGCAAA
  GCAGCATCAATAGCGCTGATGGCTACAGCTTGTATCGATATCATTTTAACGGAGAAGAGTTATTATTTGA
  GGATACATCTCTCTCCACGTCACCAGGGACAGGAGTCAGGAAAAAAACCTGTTCCTTCCGTATAAAGGAA
  GAGAAGGACGTGAAAACGCACCTAGTCCCAGAATGTCAGAACAGTCTTCACCTTTCACTGGGCACAAGCA
  CATCAGCAACCCCAGATGGCAGTCACCTTGCAGTAGATGACTTAAAGAACGCTGAAGAGTCAAAATTAGG
  TCCAGATATTGGGATTTCAAAGGAAGATGATGAAAGACAGACAGACTCTAAAAAAGAAGAAACTATTTCC
  CCAAGTTTAAATAAAACAGATGTGATACATGGACAGGACAAATCAGATGTTCAAAACACTCAGCTAACAG
  TGGAAACGACAAATATAGAAGGCACTATTTCCTATCCCCTGGAAGAAACCAAAATTACACGCTATTTCCC
  CGATGAAACGATCAATGCTTGTAAAACAATGAAGTCCAGAAGCTTCGTCTATTCCCGGGGAAGAAAGCTG
  GTCGGTGGGGTTAACCAGGATGTAGAGTACAGTTCAATCACGGACCAGCAATTGACGACGGAATGGCAAT
  GCCAAGTTCAAAAGATCACGCGCTCTCATAGCACAGATATTCCTTACATTGTGTCGGAAGCTGCAGTGCA
  AGCTGAGCATAAAGAGCAGTTTGCAGATATGCAAGATGAACACCATGTCGCTGAAGCAATTCCTCGAATC
  CCTCGCTTGTCCCTAACCATTACTGACAGAAATGGGATGGAAAACTTACTGTCTGTGAAGCCAGATCAAA
  CTTTGGGATTCCCATCTCTCAGGTCAAAAAGTTTACATGGACATCCTAGGAATGTGAAATCCATTCAGGG
  AAAGTTAGACAGATCTGGACATGCCAGTAGTGTAAGCAGCTTAGTAATTGTGTCTGGAATGACAGCAGAA
  GAAAAAAAGGTTAAGAAAGAGAAAGCTTCCACAGAAACTGAATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235323 representing NM_001252024
  Red=Cloning site Green=Tags(s)
  
  MGQKSWIEKTFCKRECIFVIPSMKDSNRCCCGQFTNQHIPPLPSATPSKNEEESKQVETQPEKWSVAKHT
  QSYPTDSYGVLEFQGGGYSNKAMYIRVSYDTKPDSLLHLMVKDWQLELPKLLISVHGGLQNFEMQPKLKQ
  VFGKGLIKAAMTTGAWIFTGGVSTGVISHVGDALKDHSSKSRGRVCAIGIAPWGIVENKEDLVGKDVTRV
  YQTMSNPLSKLSVLNNSHTHFILADNGTLGKYGAEVKLRRLLEKHISLQKINTRLGQGVPLVGLVVEGGP
  NVVSIVLEYLQEEPPIPVVICDGSGRASDILSFAHKYCEEGGIINESLREQLLVTIQKTFNYNKAQSHQL
  FAIIMECMKKKELVTVFRMGSEGQQDIEMAILTALLKGTNVSAPDQLSLALAWNRVDIARSQIFVFGPHW
  PPLGSLAPPTDSKATEKEKKPPMATTKGGRGKGKGKKKGKVKEEVEEETDPRKIELLNWVNALEQAMLDA
  LVLDRVDFVKLLIENGVNMQHFLTIPRLEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLE
  YLMGGAYRCNYTRKNFRTLYNNLFGPKRPKALKLLGMEDDEPPAKGKKKKKKKKEEEIDIDVDDPAVSRF
  QYPFHELMVWAVLMKRQKMAVFLWQRGEESMAKALVACKLYKAMAHESSESDLVDDISQDLDNNSKDFGQ
  LALELLDQSYKHDEQIAMKLLTYELKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNPG
  LKVIMGILLPPTILFLEFRTYDDFSYQTSKENEDGKEKEEENTDANADAGSRKGDEENEHKKQRSIPIGT
  KICEFYNAPIVKFWFYTISYLGYLLLFNYVILVRMDGWPSLQEWIVISYIVSLALEKIREILMSEPGKLS
  QKIKVWLQEYWNITDLVAISTFMIGAILRLQNQPYMGYGRVIYCVDIIFWYIRVLDIFGVNKYLGPYVMM
  IGKMMIDMLYFVVIMLVVLMSFGVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYAMEINP
  PCGENLYDEEGKRLPPCIPGAWLTPALMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLI
  MTFHDRPVLPPPMIILSHIYIIIMRLSGRCRKKREGDQEERDRGLKLFLSDEELKRLHEFEEQCVQEHFR
  EKEDEQQSSSDERIRVTSERVENMSMRLEEINERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGID
  RSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKE
  EKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETIS
  PSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINACKTMKSRSFVYSRGRKL
  VGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEHKEQFADMQDEHHVAEAIPRI
  PRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLHGHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAE
  EKKVKKEKASTETEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252024
• ORF Size: 4875 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252024.1, NP_001238953.1
• RefSeq Size: 5810 bp
• RefSeq ORF: 4878 bp
• Locus ID: 4308
• Cytogenetics: 15q13.3
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• MW: 185.2 kDa
• Gene Summary: 'This gene encodes a member of the transient receptor potential melastatin subfamily of transient receptor potential ion channels. The encoded protein is a calcium permeable cation channel that is expressed in melanocytes and may play a role in melanin synthesis. Specific mutations in this gene are the cause autosomal recessive complete congenital stationary night blindness-1C. The expression of this protein is inversely correlated with melanoma aggressiveness and as such it is used as a prognostic marker for melanoma metastasis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]'