Chimaerin 2 (CHN2) (NM_001293075) Human Tagged ORF Clone

CAT#: RC236861

CHN2 (myc-DDK-tagged) - Human chimerin 2 (CHN2), transcript variant 8

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHN2
• Synonyms: ARHGAP3; BCH; CHN2-3; RHOGAP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC236861 representing NM_001293075
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCTGAAGAACTGTGGCTGGAAAATGAGAAAAAGTGTGCTGTGGTTCGGAAGTCTAAGCAGGGCA
  GGAAACGCCAAGAACTGCTGGCCGTAGCCTTCGGGGTGAAGGTCCACACGTTCCGAGGCCCACACTGGTG
  TGAATATTGTGCCAATTTCATGTGGGGGCTCATCGCCCAAGGGGTCCGGTGCTCAGACTGTGGATTGAAC
  GTACACAAACAGTGTTCCAAGCACGTTCCCAATGACTGCCAACCTGATCTCAAGAGGATCAAGAAAGTGT
  ACTGTTGTGACCTCACAACACTTGTGAAGGCTCACAACACTCAGAGACCCATGGTGGTAGACATATGCAT
  TCGGGAAATTGAAGCAAGAGGATTAAAATCGGAAGGCCTTTACAGAGTCTCTGGGTTCACTGAACACATT
  GAAGATGTCAAAATGGCATTTGACAGAGAAATCTCCAATGCAGATGAGAGGCTGGAAGCCGTCCATGAAG
  TGCTGATGCTGCTGCCTCCTGCCCACTATGAAACCCTCCGGTACCTAATGATCCACCTCAAAAAGGTTAC
  TATGAATGAAAAAGACAATTTCATGAATGCAGAAAATCTGGGGATCGTGTTTGGGCCCACTCTGATGAGG
  CCCCCTGAGGACAGCACCCTGACCACCCTGCATGATATGCGGTACCAAAAGCTGATTGTGCAGATTTTAA
  TAGAAAACGAAGACGTTTTATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC236861 representing NM_001293075
  Red=Cloning site Green=Tags(s)
  
  MFSEELWLENEKKCAVVRKSKQGRKRQELLAVAFGVKVHTFRGPHWCEYCANFMWGLIAQGVRCSDCGLN
  VHKQCSKHVPNDCQPDLKRIKKVYCCDLTTLVKAHNTQRPMVVDICIREIEARGLKSEGLYRVSGFTEHI
  EDVKMAFDREISNADERLEAVHEVLMLLPPAHYETLRYLMIHLKKVTMNEKDNFMNAENLGIVFGPTLMR
  PPEDSTLTTLHDMRYQKLIVQILIENEDVLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293075
• ORF Size: 723 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293075.1, NP_001280004.1
• RefSeq Size: 2742 bp
• RefSeq ORF: 726 bp
• Locus ID: 1124
• Cytogenetics: 7p14.3
• MW: 28.4 kDa
• Gene Summary: 'This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that contains a phorbol-ester/diacylglycerol (DAG)-type zinc finger, a Rho-GAP domain, and an SH2 domain. The encoded protein translocates from the cytosol to the Golgi apparatus membrane upon binding by diacylglycerol (DAG). Activity of this protein is important in cell proliferation and migration, and expression changes in this gene have been detected in cancers. A mutation in this gene has also been associated with schizophrenia in men. Alternative transcript splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, May 2014]'