POLR3F (NM_001282526) Human Tagged ORF Clone

CAT#: RC237089

POLR3F (myc-DDK-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa (POLR3F), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POLR3F
• Synonyms: RPC6; RPC39
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237089 representing NM_001282526
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCATATAGAAGCCCAGCAGCGGGCAGTAGCCATCAATAGGTTGTTGTCTATGGGTCAGTTGGATC
  TCTTAAGGAGCAATACGGGCCTTTTATATAGAATAAAGGACTCTCAGAATGCTGGTAAAATGAAGGGATC
  CGATAACCAAGAAAAACTAGTATATCAAATCATAGAGGATGCAGGAAATAAAGGAATATGGAGCAGAGAT
  ATCCGCTATAAAAGTAATTTGCCATTAACAGAAATCAACAAAATTCTGAAGAATCTGGAAAGTAAAAAGC
  TTATCAAAGCTGTTAAGTCTGTAGCAGCCTCAAAAAAGAAGGTGTATATGCTCTATAACCTGCAGCCAGA
  CCGGTCTGTGACTGGTGGAGCCTGGTACAGTGACCAGGATTTTGAATCTGAATTTGTAGAGGTGCTTAAC
  CAACAGTGTTTTAAATTCCTACAGTCCAAGGCAGAAACAGCACGAGAAAGCAAACAGAACCCAATGATAC
  AAAGAAATAGTTCATTTGCCTCATCACATGAAGTGTGGAAATATATCTGCGAATTGGGAATCAGTAAGGT
  AGAGTTATCCATGGAAGACATTGAAACCATCCTGAATACACTCATTTATGATGGAAAAGTGGAGATGACG
  ATTATTGCTGCAAAAGAAGGCACAGTTGGCAGTGTAGATGGACACATGAAACTGTACAGGGCAGTCAATC
  CAATCATCCCTCCCACAGGTTTGGTCCGGGCACCCTGTGGACTCTGCCCGGTTTTTGATGACTGCCACGA
  AGGTGGTGAGATTTCACCATCTAACTGTATTTACATGACAGAGTGGCTCGAATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237089 representing NM_001282526
  Red=Cloning site Green=Tags(s)
  
  MPHIEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRD
  IRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLN
  QQCFKFLQSKAETARESKQNPMIQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMT
  IIAAKEGTVGSVDGHMKLYRAVNPIIPPTGLVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282526
• ORF Size: 825 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001282526.1, NP_001269455.1
• RefSeq Size: 2262
• RefSeq ORF: 828
• Locus ID: 10621
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• MW: 31.5 kDa
• Gene Summary: The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]