SH3GL3 (NM_001301108) Human Tagged ORF Clone

CAT#: RC237118

SH3GL3 (myc-DDK-tagged) - Human SH3-domain GRB2-like 3 (SH3GL3), transcript variant EEN-B2-L2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SH3GL3
• Synonyms: CNSA3; EEN-B2; HsT19371; SH3D2C; SH3P13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237118 representing NM_001301108
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAACACTGTGTCGAAGATCCGAGGGCAGGTGAAGACCACAGGATACCCGCAGACGGAAGGCTTGC
  TGGGGGACTGTATGCTGAAATACGGGAAGGAGCTCGGGGAAGACTCCACCTTTGGCAATGCATTGATAGA
  AGTTGGTGAATCCATGAAGCTAATGGCTGAGGTGAAAGACTCTCTTGATATTAATGTAAAGCAAACTTTT
  ATTGATCCACTTCAGTTACTACAAGATAAAGATTTAAAAGAGATCGGGCATCACCTGAAAAAGCTGGAAG
  GCCGCCGCCTGGATTACGATTATAAAAAGAAACGAGTAGGTAAGATACCAGACGAAGAAGTCAGACAAGC
  GGTAGAAAAATTTGAAGAGTCAAAGGAGTTGGCTGAAAGAAGCATGTTTAACTTTTTAGAAAATGATGTA
  GAACAAGTCAGCCAGTTGGCTGTGTTCATAGAGGCAGCATTAGACTATCACAGACAGTCCACAGAGATTC
  TGCAGGAGCTGCAGAGCAAGCTACAGATGCGAATATCAGCTGCATCCAGTGTCCCCAGACGAGAATACAA
  GCCAAGGCCTGTGAAAAGGAGTTCTAGTGAGCTCAATGGAGTTTCCACCACCTCTGTAGTGAAGACGACA
  GGTTCTAACATTCCCATGGACCAGCCCTGCTGTCGTGGTCTCTATGACTTTGAGCCAGAAAACCAAGGAG
  AATTAGGATTTAAAGAAGGGGACATCATTACATTAACCAATCAAATAGATGAAAACTGGTATGAAGGAAT
  GATACACGGAGAATCGGGATTCTTCCCCATTAATTACGTGGAAGTGATCGTGCCTTTACCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237118 representing NM_001301108
  Red=Cloning site Green=Tags(s)
  
  MLNTVSKIRGQVKTTGYPQTEGLLGDCMLKYGKELGEDSTFGNALIEVGESMKLMAEVKDSLDINVKQTF
  IDPLQLLQDKDLKEIGHHLKKLEGRRLDYDYKKKRVGKIPDEEVRQAVEKFEESKELAERSMFNFLENDV
  EQVSQLAVFIEAALDYHRQSTEILQELQSKLQMRISAASSVPRREYKPRPVKRSSSELNGVSTTSVVKTT
  GSNIPMDQPCCRGLYDFEPENQGELGFKEGDIITLTNQIDENWYEGMIHGESGFFPINYVEVIVPLPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301108
• ORF Size: 834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301108.1, NP_001288037.1
• RefSeq Size: 1836 bp
• RefSeq ORF: 837 bp
• Locus ID: 6457
• Cytogenetics: 15q25.2
• Protein Pathways: Endocytosis
• MW: 32 kDa
• Gene Summary: ''