SH3GL3 (NM_001301108) Human Untagged Clone

CAT#: SC335012

SH3GL3 (untagged) - Human SH3-domain GRB2-like 3 (SH3GL3), transcript variant EEN-B2-L2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SH3GL3
• Synonyms: CNSA3; EEN-B2; HsT19371; SH3D2C; SH3P13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335012 representing NM_001301108.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGAACACTGTGTCGAAGATCCGAGGGCAGGTGAAGACCACAGGATACCCGCAGACGGAAGGCTTG
  CTGGGGGACTGTATGCTGAAATACGGGAAGGAGCTCGGGGAAGACTCCACCTTTGGCAATGCATTGATA
  GAAGTTGGTGAATCCATGAAGCTAATGGCTGAGGTGAAAGACTCTCTTGATATTAATGTAAAGCAAACT
  TTTATTGATCCACTTCAGTTACTACAAGATAAAGATTTAAAAGAGATCGGGCATCACCTGAAAAAGCTG
  GAAGGCCGCCGCCTGGATTACGATTATAAAAAGAAACGAGTAGGTAAGATACCAGACGAAGAAGTCAGA
  CAAGCGGTAGAAAAATTTGAAGAGTCAAAGGAGTTGGCTGAAAGAAGCATGTTTAACTTTTTAGAAAAT
  GATGTAGAACAAGTCAGCCAGTTGGCTGTGTTCATAGAGGCAGCATTAGACTATCACAGACAGTCCACA
  GAGATTCTGCAGGAGCTGCAGAGCAAGCTACAGATGCGAATATCAGCTGCATCCAGTGTCCCCAGACGA
  GAATACAAGCCAAGGCCTGTGAAAAGGAGTTCTAGTGAGCTCAATGGAGTTTCCACCACCTCTGTAGTG
  AAGACGACAGGTTCTAACATTCCCATGGACCAGCCCTGCTGTCGTGGTCTCTATGACTTTGAGCCAGAA
  AACCAAGGAGAATTAGGATTTAAAGAAGGGGACATCATTACATTAACCAATCAAATAGATGAAAACTGG
  TATGAAGGAATGATACACGGAGAATCGGGATTCTTCCCCATTAATTACGTGGAAGTGATCGTGCCTTTA
  CCTCAGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001301108
• Insert Size: 837 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301108.1
• RefSeq Size: 1836 bp
• RefSeq ORF: 837 bp
• Locus ID: 6457
• UniProt ID: Q99963
• Cytogenetics: 15q25.2
• Protein Pathways: Endocytosis
• MW: 31.6 kDa
• Gene Summary: Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (EEN-B2-L2) uses an alternate splice site, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant EEN-B2-L1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Variants 2, 9, and 14 all encode the same isoform (b).