DH5α Chemically Competent E. coli Cells

CAT#: CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

Specifications

Product Data

• Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk -, mk +) supE44 thi-1 phoA Δ(lacZYA argF)U169 Φ80lacZΔM15 λ-DNA Efficiency pUC19 - 2.7Kb / 1 x 10^8 cfu/ug
• Components: Positive Control DNA - pUC19 (10pg/μl)
  SOC medium – 20ml
• Features: Chemically Competent Accommodates small and larger plasmids RecA1 and endA1 markers for minimal recombination and enhanced quality of plasmid DNA
• Applications: lacZ marker for blue/white color screening
  Transformation of cloned DNA into bacterial cells
  Ideal for subcloning and generating cDNA libraries
• Storage: The Competent Cells should be stored at -80°C. pUC19 Control DNA should be stored at -20°C and SOC recovery medium should be stored at 4°C immediately upon arrival.
• Stability: The cells are stable for 6 months from the date of shipment.