TAL1 (NM_001290403) Human Tagged ORF Clone

CAT#: RC237495

TAL1 (myc-DDK-tagged) - Human T-cell acute lymphocytic leukemia 1 (TAL1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAL1
• Synonyms: bHLHa17; SCL; tal-1; TCL5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237495 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGACCGAGCGGCCGCCGAGCGAGGCGGCTCGCAGTGACCCCCAGCTAGAGGGACGGGACGCGGCCGA
  GGCCAGCATGGCCCCCCCGCACCTGGTCCTGCTGAACGGCGTCGCCAAGGAGACGAGCCGCGCGGCCGCA
  GCGGAGCCCCCAGTCATCGAACTGGGCGCGCGCGGAGGCCCGGGGGGCGGCCCTGCCGGTGGGGGCGGCG
  CCGCGAGAGACTTAAAGGGCCGCGACGCGGCGACGGCCGAAGCGCGCCATCGGGTGCCCACCACCGAGCT
  GTGCAGACCTCCCGGGCCCGCCCCGGCCCCCGCGCCCGCCTCGGTTACAGCGGAGCTGCCCGGCGACGGC
  CGCATGGTGCAGCTGAGTCCTCCCGCGCTGGCTGCCCCCGCCGCCCCCGGCCGCGCGCTGCTCTACAGCC
  TCAGCCAGCCGCTGGCCTCTCTCGGCAGCGGGTTCTTTGGGGAGCCGGATGCCTTCCCTATGTTCACCAC
  CAACAATCGAGTGAAGAGGAGACCTTCCCCCTATGAGATGGAGATTACTGATGGTCCCCACACCAAAGTT
  GTGCGGCGTATCTTCACCAACAGCCGGGAGCGATGGCGGCAGCAGAATGTGAACGGGGCCTTTGCCGAGC
  TCCGCAAGCTGATCCCCACACATCCCCCGGACAAGAAGCTCAGCAAGAATGAGATCCTCCGCCTGGCCAT
  GAAGTATATCAACTTCTTGGCCAAGCTGCTCAATGACCAGGAGGAGGAGGGCACCCAGCGGGCCAAGACT
  GGCAAGGACCCTGTGGTGGGGGCTGGTGGGGGTGGAGGTGGGGGAGGGGGTGGCGCGCCCCCAGATGACC
  TCCTGCAAGACGTGCTTTCCCCCAACTCCAGCTGCGGCAGCTCCCTGGATGGGGCAGCCAGCCCGGACAG
  CTACACGGAGGAGCCCGCGCCCAAGCACACGGCCCGCAGCCTCCATCCTGCCATGCTGCCTGCCGCCGAT
  GGAGCCGGCCCTCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237495 protein sequence
  Red=Cloning site Green=Tags(s)
  
  X*PSGRRARRLAVTPS*RDGTRPRPAWPPRTWSC*TASPRRRAARPQRSPQSSNWARAEARGAALPVGAA
  PRET*RAATRRRPKRAIGCPPPSCADLPGPPRPPRPPRLQRSCPATAAWCS*VLPRWLPPPPPAARCSTA
  SASRWPLSAAGSLGSRMPSLCSPPTIE*RGDLPPMRWRLLMVPTPKLCGVSSPTAGSDGGSRM*TGPLPS
  SAS*SPHIPRTRSSARMRSSAWP*SISTSWPSCSMTRRRRAPSGPRLARTLWWGLVGVEVGEGVARPQMT
  SCKTCFPPTPAAAAPWMGQPARTATRRSPRPSTRPAASILPCCLPPMEPAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290403
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290403.1, NP_001277332.1
• RefSeq Size: 4774 bp
• RefSeq ORF: 996 bp
• Locus ID: 6886
• Cytogenetics: 1p33
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• MW: 34.3 kDa
• Gene Summary: ''