TXNRD2 (NM_001282512) Human Tagged ORF Clone

CAT#: RC237559

TXNRD2 (myc-DDK-tagged) - Human thioredoxin reductase 2 (TXNRD2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TXNRD2
• Synonyms: GCCD5; SELZ; TR; TR-BETA; TR3; TRXR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237559 representing NM_001282512
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAATGGCGGTGGCGCTGCGGGGATTAGGAGGGCGCTTCCGGTGGCGGACGCAGGCCGTGGCGG
  GCGGGGTGCGGGGCGCGGCGCGGGGCGCAGCAGCAGGTCAGCGGGACTATGATCTCCTGGTGGTCGGCGG
  GGGATCTGGTGGCCTGGCTTGTGCCAAGGAGGCCGCCCAGCTGGGAAGGAAGGTGGCCGTGGTGGACTAC
  GTGGAACCTTCTCCCCAAGGCACCCGGTGGGGCCTCGGCGGCACCTGCGTCAACGTGGGCTGCATCCCCA
  AGAAGCTGATGCACCAGGCGGCACTGCTGGGAGGCCTGATCCAAGATGCCCCCAACTATGGCTGGGAGGT
  GGCCCAGCCCGTGCCGCATGACTGGAGGAAGATGGCAGAAGCTGTTCAAAATCACGTGAAATCCTTGAAC
  TGGGGCCACCGTGTCCAGCTTCAGGACAGAAAAGTCAAGTACTTTAACATCAAAGCCAGCTTTGTTGACG
  AGCACACGGTTTGCGGCGTTGCCAAAGGTGGGAAAGAGATTCTGCTGTCAGCCGATCACATCATCATTGC
  TACTGGAGGGCGGCCGAGATACCCCACGCACATCGAAGGTGCCTTGGAATATGGAATCACAAGTGATGAC
  ATCTTCTGGCTGAAGGAATCCCCTGGAAAAACGTTGGTGGTCGGGGCCAGCTATGTGGCCCTGGAGTGTG
  CTGGCTTCCTCACCGGGATTGGGCTGGACACCACCATCATGATGCGCAGCATCCCCCTCCGCGGCTTCGA
  CCAGCAAATGTCCTCCATGGTCATAGAGCACATGGCATCTCATGGCACCCGGTTCCTGAGGGGCTGTGCC
  CCCTCGCGGGTCAGGAGGCTCCCTGATGGCCAGCTGCAGGTCACCTGGGAGGACAGCACCACCGGCAAGG
  AGGACACGGGCACCTTTGACACCGTCCTGTGGGCCATAGCACCTTGCATCTCTGCGTGTCTCCCCACCAC
  CGTGGGACATGCTGGAAAAAACCAGAGAAGAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237559 representing NM_001282512
  Red=Cloning site Green=Tags(s)
  
  MAAMAVALRGLGGRFRWRTQAVAGGVRGAARGAAAGQRDYDLLVVGGGSGGLACAKEAAQLGRKVAVVDY
  VEPSPQGTRWGLGGTCVNVGCIPKKLMHQAALLGGLIQDAPNYGWEVAQPVPHDWRKMAEAVQNHVKSLN
  WGHRVQLQDRKVKYFNIKASFVDEHTVCGVAKGGKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDD
  IFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGTRFLRGCA
  PSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIAPCISACLPTTVGHAGKNQRRD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282512
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001282512.1, NP_001269441.1
• RefSeq Size: 2081
• RefSeq ORF: 1017
• Locus ID: 10587
• Protein Families: Druggable Genome
• Protein Pathways: Pyrimidine metabolism
• MW: 36.8 kDa
• Gene Summary: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes a mitochondrial form important for scavenging reactive oxygen species in mitochondria. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants encoding different isoforms, including a few localized in the cytosol and some lacking the C-terminal Sec residue, have been found for this gene. [provided by RefSeq, Jun 2017]