PRAF1 (POLR1E) (NM_001282766) Human Tagged ORF Clone

CAT#: RC237633

POLR1E (myc-DDK-tagged) - Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POLR1E
• Synonyms: PAF53; PRAF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237633 representing NM_001282766
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTGAATTGTTCAACATGCAGCCACTATTTTCAGGTCTTTCACCAAGAAAGCAAAATTATTTTCT
  GGAAAGAGCCAAGGACTTGTTTTCTAATCCTGTCTCTGCCACTACCTGGTGAACTGACCTGGGATGTATC
  AGTTGAGAGTGAACTGGCGCTAGAGAGTCAGACCAAAACTTACAGAGAAAAGATGGATTCTTGTATTGAA
  GCCTTTGGTACCACCAAACAGAAGCGAGCTCTGAACACCAGGAGAATGAACAGAGTTGGCAATGAATCTT
  TGAATCGTGCAGTGGCTAAAGCTGCAGAGACTATCATTGATACGAAGGGTGTGACTGCTCTGGTCAGCGA
  TGCTATCCACAATGACTTGCAAGATGACTCCCTCTACCTTCCTCCCTGCTATGATGATGCAGCCAAGCCT
  GAAGACGTGTATAAATTTGAAGATCTTCTTTCCCCTGCGGAGTATGAAGCTCTTCAGAGCCCATCTGAAG
  CTTTCAGGAACGTCACGTCAGAAGAAATACTGAAGATGATTGAGGAGAACAGCCATTGCACCTTTGTCAT
  AGAAGCGTTGAAGTCTTTGCCATCAGATGTGGAGAGCCGAGACCGCCAGGCCCGATGCATATGGTTTCTG
  GATACCCTCATCAAATTTCGAGCTCATAGGGTAGTTAAGCGGAAAAGTGCTCTGGGACCTGGAGTTCCCC
  ACATCATCAACACCAAACTGCTGAAGCACTTTACTTGCTTGACCTACAACAATGGCAGATTACGGAACTT
  AATTTCGGATTCTATGAAGGCGAAGATTACTGCATATGTGATCATACTTGCCTTGCACATACATGACTTC
  CAAATTGACCTGACAGTGTTACAGAGGGACTTGAAGCTCAGTGAGAAAAGGATGATGGAGATAGCCAAAG
  CCATGAGGCTGAAGATCTCCAAAAGAAGGGTGTCTGTGGCCGCCGGCAGTGAAGAAGATCACAAACTGGG
  CACCCTGTCCCTCCCGCTGCCTCCAGCCCAGACCTCAGACCGCCTGGCAAAGCGGAGGAAGATTACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237633 representing NM_001282766
  Red=Cloning site Green=Tags(s)
  
  MMLNCSTCSHYFQVFHQESKIIFWKEPRTCFLILSLPLPGELTWDVSVESELALESQTKTYREKMDSCIE
  AFGTTKQKRALNTRRMNRVGNESLNRAVAKAAETIIDTKGVTALVSDAIHNDLQDDSLYLPPCYDDAAKP
  EDVYKFEDLLSPAEYEALQSPSEAFRNVTSEEILKMIEENSHCTFVIEALKSLPSDVESRDRQARCIWFL
  DTLIKFRAHRVVKRKSALGPGVPHIINTKLLKHFTCLTYNNGRLRNLISDSMKAKITAYVIILALHIHDF
  QIDLTVLQRDLKLSEKRMMEIAKAMRLKISKRRVSVAAGSEEDHKLGTLSLPLPPAQTSDRLAKRRKIT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282766
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001282766.1, NP_001269695.1
• RefSeq Size: 1978
• RefSeq ORF: 1050
• Locus ID: 64425
• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• MW: 40.2 kDa