LIPI (NM_001303001) Human Tagged ORF Clone

CAT#: RC237829

LIPI (myc-DDK-tagged) - Human lipase, member I (LIPI), transcript variant deltaE8

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LIPI
• Synonyms: CT17; LPDL; mPA-PLA1 beta; PLA1C; PRED5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237829 representing NM_001303001
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGTATACATTTTTCTTTGTTTGATGTGCTGGGTGAGATCTGATAATAAAAGACCATGCCTTGAAT
  TCTCTCAGCTAAGTGTAAAGGATTCCTTCAGAGATTTATTTATTCCGAGAATAGAGACCATTCTGATGAT
  GTATACAAGGAACAACCTAAACTGTGCTGAGCCACTGTTTGAACAAAATAACTCACTTAATGTTAATTTC
  AACACACAAAAGAAAACAGTCTGGCTTATTCACGGATACAGACCAGTAGGCTCCATCCCATTATGGCTTC
  AGAACTTCGTAAGGATTTTGCTGAATGAAGAAGATATGAATGTAATTGTAGTAGACTGGAGCCGGGGTGC
  TACAACTTTTATTTATAATAGAGCAGTTAAAAACACCAGAAAAGTTGCTGTGAGTTTGAGTGTGCACATT
  AAAAATCTTTTGAAGCATGGTGCATCTCTTGACAATTTTCATTTCATAGGTGTGAGCTTAGGGGCTCATA
  TCAGTGGATTTGTTGGAAAGATATTTCATGGTCAACTTGGAAGAATAACAGGTCTTGACCCTGCTGGGCC
  AAGGTTCTCCAGAAAACCACCATATAGCAGATTAGATTACACGGATGCAAAGTTTGTGGATGTCATCCAT
  TCTGACTCCAATGGTTTAGGCATTCAAGAGCCCTTGGGACATATAGATTTTTATCCAAATGGAGGAAATA
  AACAACCTGGCTGTCCTAAATCAATTTTCTCAGGAATTCAATTCATTAAATGCAACCACCAGAGAGCAGT
  TCACTTGTTCATGGCATCTTTAGAAACAAACTGCAATTTTATTTCATTTCCTTGTCGTTCATACAAAGAT
  TACAAGACTAGCTTATGTGTGGACTGTGACTGTTTTAAGGAAAAATCATGTCCTCGGCTGGGTTATCAAG
  CCAAGCTATTTAAAGGTGTTTTAAAAGAAAGGATGGAAGGAAGACCTCTTAGGACCACTGTGTTTTTGGA
  TACAAGTGGTACATATCCATTCTGTAACCACCACTTTGCAGGTATAATATTGTACTTAAAGACAGAGAGG
  AAGTGTTTCTTAATCCAAACACATGTACACCAAAGAACACATAAGATGCCTTCTTCCATCAAATGCACTT
  GCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237829 representing NM_001303001
  Red=Cloning site Green=Tags(s)
  
  MRVYIFLCLMCWVRSDNKRPCLEFSQLSVKDSFRDLFIPRIETILMMYTRNNLNCAEPLFEQNNSLNVNF
  NTQKKTVWLIHGYRPVGSIPLWLQNFVRILLNEEDMNVIVVDWSRGATTFIYNRAVKNTRKVAVSLSVHI
  KNLLKHGASLDNFHFIGVSLGAHISGFVGKIFHGQLGRITGLDPAGPRFSRKPPYSRLDYTDAKFVDVIH
  SDSNGLGIQEPLGHIDFYPNGGNKQPGCPKSIFSGIQFIKCNHQRAVHLFMASLETNCNFISFPCRSYKD
  YKTSLCVDCDCFKEKSCPRLGYQAKLFKGVLKERMEGRPLRTTVFLDTSGTYPFCNHHFAGIILYLKTER
  KCFLIQTHVHQRTHKMPSSIKCTCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303001
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001303001.1, NP_001289930.1
• RefSeq Size: 1320
• RefSeq ORF: 1128
• Locus ID: 149998
• Protein Families: Secreted Protein
• MW: 43.4 kDa
• Gene Summary: The protein encoded by this gene is a phospholipase that hydrolyzes phosphatidic acid to produce lysophosphatidic acid. Defects in this gene are a cause of susceptibility to familial hypertrigliceridemia. This gene is also expressed at high levels in Ewing family tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]