VPS45A (VPS45) (NM_001279355) Human Tagged ORF Clone

CAT#: RC238285

VPS45 (myc-DDK-tagged) - Human vacuolar protein sorting 45 homolog (S. cerevisiae) (VPS45), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VPS45
• Synonyms: H1; H1VPS45; SCN5; VPS45A; VPS45B; VPS54A; VSP45; VSP45A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238285 representing NM_001279355
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGTGGTTTTTGCTGTGAAGCAGTACCCACATTTGTTTTCCCTCAATATTTTGGGTTGCTGCCAGG
  GTCGAAATTGGGATCCAGCCCAGCTATCTAGAACAACTCAAGGGCTTACAGCTCTCCTTTTATCTCTGAA
  GAAGTGTCCCATGATTCGTTATCAGCTCTCATCAGAGGCAGCAAAGAGACTTGCAGAGTGCGTTAAGCAA
  GTGATAACTAAAGAATATGAACTGTTTGAATTCCGTCGGACAGAGGTTCCTCCATTGCTCCTTATTTTAG
  ATCGCTGTGATGATGCCATCACCCCATTGCTAAACCAGTGGACATATCAGGCCATGGTCCACGAACTACT
  AGGCATAAACAACAATCGGATTGATCTTTCCAGAGTGCCGGGAATCAGTAAAGACTTAAGAGAAGTGGTC
  CTATCTGCTGAAAATGATGAATTCTATGCTAATAATATGTACCTGAACTTTGCTGAGATTGGTAGCAATA
  TAAAGAATCTCATGGAAGATTTTCAGAAGAAGAAACCAAAAGAACAGCAAAAACTAGAATCAATAGCAGA
  CATGAAGGCGTTTGTTGAGAATTATCCACAGTTCAAGAAAATGTCTGGGACTGTTTCAAAGCATGTGACA
  GTGGTTGGAGAACTGTCTCGATTGGTCAGTGAACGGAATCTGCTGGAGGTTTCAGAGGTTGAGCAAGAAC
  TGGCCTGTCAAAATGACCATTCTAGTGCTCTCCAGAATATAAAAAGGCTTCTGCAGAACCCCAAAGTGAC
  AGAGTTTGATGCTGCCCGCCTGGTGATGCTTTATGCTTTACATTATGAGCGACACAGCAGCAATAGCCTG
  CCAGGACTAATGATGGACCTCAGGAATAAAGGTGTTTCTGAGAAGTATCGAAAGCTCGTGTCTGCAGTTG
  TTGAATATGGTGGTAAACGAGTCAGAGGAAGTGACCTCTTCAGCCCCAAAGATGCTGTGGCTATCACCAA
  ACAATTCCTCAAAGGACTGAAGGGAGTAGAAAATGTATATACACAGCATCAACCTTTCCTACATGAAACC
  CTGGATCATCTCATCAAAGGAAGGCTTAAGGAAAACCTATATCCTTATTTAGGCCCCAGCACACTCAGAG
  ACAGACCTCAGGATATCATTGTGTTTGTAATTGGAGGAGCCACCTATGAAGAGGCTCTAACAGTTTATAA
  CCTGAACCGCACCACTCCTGGAGTGAGGATTGTCCTGGGAGGCACCACAGTGCACAACACGAAAAGTTTC
  CTAGAGGAAGTTCTGGCTTCTGGACTGCACAGCCGAAGCAAGGAGAGCTCTCAAGTCACATCAAGGTCAG
  CGAGCAGAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238285 representing NM_001279355
  Red=Cloning site Green=Tags(s)
  
  MNVVFAVKQYPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLAECVKQ
  VITKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVV
  LSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVT
  VVGELSRLVSERNLLEVSEVEQELACQNDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSL
  PGLMMDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVAITKQFLKGLKGVENVYTQHQPFLHET
  LDHLIKGRLKENLYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSF
  LEEVLASGLHSRSKESSQVTSRSASRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001279355
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001279355.1, NP_001266284.1
• RefSeq Size: 2080
• RefSeq ORF: 1344
• Locus ID: 11311
• Protein Pathways: Endocytosis
• MW: 51.2 kDa
• Gene Summary: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013]