KIR5.1 (KCNJ16) (NM_001291623) Human Tagged ORF Clone

CAT#: RC238309

KCNJ16 (myc-DDK-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 16 (KCNJ16), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNJ16
• Synonyms: BIR9; KIR5.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238309 representing NM_001291623
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAAAAATGGTTCTAACTGAAAACCCAAACCAAGAAATAGCAACAAGTCTAGAATTCTTACTACTAC
  AAAACTCACCTGGATCCCTAAGGGCACAGCAAAGAATGAGCTATTACGGCAGCAGCTATCATATTATCAA
  TGCGGACGCAAAATACCCAGGCTACCCGCCAGAGCACATTATAGCTGAGAAGAGAAGAGCAAGAAGACGA
  TTACTTCACAAAGATGGCAGCTGTAATGTCTACTTCAAGCACATTTTTGGAGAATGGGGAAGCTATGTGG
  TTGACATCTTCACCACTCTTGTGGACACCAAGTGGCGCCATATGTTTGTGATATTTTCTTTATCTTATAT
  TCTCTCGTGGTTGATATTTGGCTCTGTCTTTTGGCTCATAGCCTTTCATCATGGCGATCTATTAAATGAT
  CCAGACATCACACCTTGTGTTGACAACGTCCATTCTTTCACAGGGGCCTTTTTGTTCTCCCTAGAGACCC
  AAACCACCATAGGATATGGTTATCGCTGTGTTACTGAAGAATGTTCTGTGGCCGTGCTCATGGTGATCCT
  CCAGTCCATCTTAAGTTGCATCATAAATACCTTTATCATTGGAGCTGCCTTGGCCAAAATGGCAACTGCT
  CGAAAGAGAGCCCAAACCATTCGTTTCAGCTACTTTGCACTTATAGGTATGAGAGATGGGAAGCTTTGCC
  TCATGTGGCGCATTGGTGATTTTCGGCCAAACCACGTGGTAGAAGGAACAGTTAGAGCCCAACTTCTCCG
  CTATACAGAAGACAGTGAAGGGAGGATGACGATGGCATTTAAAGACCTCAAATTAGTCAACGACCAAATC
  ATCCTGGTCACCCCGGTAACTATTGTCCATGAAATTGACCATGAGAGCCCTCTGTATGCCCTTGACCGCA
  AAGCAGTAGCCAAAGATAACTTTGAGATTTTGGTGACATTTATCTATACTGGTGATTCCACTGGAACATC
  TCACCAATCTAGAAGCTCCTATGTTCCCCGAGAAATTCTCTGGGGCCATAGGTTTAATGATGTCTTGGAA
  GTTAAGAGGAAGTATTACAAAGTGAACTGCTTACAGTTTGAAGGAAGTGTGGAAGTATATGCCCCCTTTT
  GCAGTGCCAAGCAATTGGACTGGAAAGACCAGCAGCTCCACATAGAAAAAGCACCACCAGTTCGAGAATC
  CTGCACGTCGGACACCAAGGCGAGACGAAGGTCATTTAGTGCAGTTGCCATTGTCAGCAGCTGTGAAAAC
  CCTGAGGAGACCACCACTTCCGCCACACATGAATATAGGGAAACACCTTATCAGAAAGCTCTCCTGACTT
  TAAACAGAATCTCTGTAGAATCCCAAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238309 representing NM_001291623
  Red=Cloning site Green=Tags(s)
  
  MLKMVLTENPNQEIATSLEFLLLQNSPGSLRAQQRMSYYGSSYHIINADAKYPGYPPEHIIAEKRRARRR
  LLHKDGSCNVYFKHIFGEWGSYVVDIFTTLVDTKWRHMFVIFSLSYILSWLIFGSVFWLIAFHHGDLLND
  PDITPCVDNVHSFTGAFLFSLETQTTIGYGYRCVTEECSVAVLMVILQSILSCIINTFIIGAALAKMATA
  RKRAQTIRFSYFALIGMRDGKLCLMWRIGDFRPNHVVEGTVRAQLLRYTEDSEGRMTMAFKDLKLVNDQI
  ILVTPVTIVHEIDHESPLYALDRKAVAKDNFEILVTFIYTGDSTGTSHQSRSSYVPREILWGHRFNDVLE
  VKRKYYKVNCLQFEGSVEVYAPFCSAKQLDWKDQQLHIEKAPPVRESCTSDTKARRRSFSAVAIVSSCEN
  PEETTTSATHEYRETPYQKALLTLNRISVESQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291623
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291623.1, NP_001278552.1
• RefSeq Size: 3956 bp
• RefSeq ORF: 1362 bp
• Locus ID: 3773
• Cytogenetics: 17q24.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 52.4 kDa
• Gene Summary: 'Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which tends to allow potassium to flow into rather than out of a cell, can form heterodimers with two other inward-rectifier type potassium channels. It may function in fluid and pH balance regulation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014]'