MAP3K19 (NM_001018047) Human Tagged ORF Clone

CAT#: RC238373

  • TrueORF®

MAP3K19 (myc-DDK-tagged) - Human mitogen-activated protein kinase kinase kinase 19 (MAP3K19), transcript variant 4


  "NM_001018047" in other vectors (1)

Reconstitution Protocol

USD 420.00

2 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MAP3K19
Synonyms RCK; YSK4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC238373 representing NM_001018047
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTCTATGCCAAAACCAGAAAGACATGCTGAGTCATTGCTTGACATTTGTCATGATACAAACTCTT
CTCCAACTGATTTGATGACAGTTACCAAAAATCAAAACATCATCTTGCAAAGCATCAGCAGAAGTGAGGA
GTTCGACCAAGATGGTGACTGCAGTCATTCCACACTGGTTAATGAAGAAGAAGATCCCAGTGGTGGTAGA
CAGGACTGGCAACCCAGGACAGAAGGTGTTGAGATCACTGTAACTTTTCCAAGAGATGTCAGTCCTCCCC
AAGAAATGAGCCAAGAAGACTTAAAAGAAAAGAATCTGATAAACTCATCGCTTCAAGAATGGGCACAAGC
ACATGCAGTTTCTCATCCAAATGAAATAGAAACGGTGGAGCTCAGGAAAAAGAAGCTGACCATGCGGCCC
TTAGTTTTGCAAAAAGAGGAAAGTTCCAGGGAGCTCTGCAATGTGAACTTGGGCTTTTTGCTACCAAGAT
CTTGTTTAGAACTGAACATTTCCAAGTCTGTAACCAGAGAAGATGCTCCTCATTTTCTGAAGGAGCAGCA
AAGAAAATCTGAAGAGTTTTCGACCTCTCATATGAAGTACAGTGGCCGAAGCATCAAGAGGCATAGTAGT
GGGCTCAGGATATATGACAGGGAGGAGAAATTTCTCATCTCAAATGAAAAGAAGATATTTTCTGAAAATA
GTTTAAAGTCTGAAGAACCTATCCTATGGACCAAGGTAGATTTGCTCAAAGCACTGAAACATGTCAACAT
TGTGGCCTATTTGGGGACATGCTTGCAAGAGAACACTGTGAGCATTTTCATGGAGTTTGTTCCTGGTGGC
TCAATCTCTAGTATTATAAACCGTTTTGGGCCATTGCCTGAGATGGTGTTCTGTAAATATACGAAACAAA
TACTTCAAGGTGTTGCTTATCTCCATGAGAACTGTGTGGTACATCGCGATATCAAAGGAAATAATGTTAT
GCTCATGCCAACTGGAATAATAAAGCTGATTGACTTTGGCTGTGCCAGGCGTTTGGCCTGGGCAGGTTTA
AATGGCACCCACAGTGACATGCTTAAGTCCATGCATGGGACTCCATATTGGATGGCCCCAGAAGTCATCA
ATGAGTCTGGCTATGGACGGAAATCAGATATCTGGAGCATTGGTTGTACTGTGTTTGAGATGGCTACAGG
GAAGCCTCCACTGGCTTCCATGGACAGGATGGCCGCCATGTTTTACATCGGAGCACACCGAGGGCTGATG
CCTCCTTTACCAGACCACTTCTCAGAAAATGCAGCAGACTTTGTGCGCATGTGCCTGACCAGGGACCAGC
ATGAGCGACCTTCTGCTCTCCAGCTCCTGAAGCACTCCTTCTTGGAGAGAAGTCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC238373 representing NM_001018047
Red=Cloning site Green=Tags(s)

MSSMPKPERHAESLLDICHDTNSSPTDLMTVTKNQNIILQSISRSEEFDQDGDCSHSTLVNEEEDPSGGR
QDWQPRTEGVEITVTFPRDVSPPQEMSQEDLKEKNLINSSLQEWAQAHAVSHPNEIETVELRKKKLTMRP
LVLQKEESSRELCNVNLGFLLPRSCLELNISKSVTREDAPHFLKEQQRKSEEFSTSHMKYSGRSIKRHSS
GLRIYDREEKFLISNEKKIFSENSLKSEEPILWTKVDLLKALKHVNIVAYLGTCLQENTVSIFMEFVPGG
SISSIINRFGPLPEMVFCKYTKQILQGVAYLHENCVVHRDIKGNNVMLMPTGIIKLIDFGCARRLAWAGL
NGTHSDMLKSMHGTPYWMAPEVINESGYGRKSDIWSIGCTVFEMATGKPPLASMDRMAAMFYIGAHRGLM
PPLPDHFSENAADFVRMCLTRDQHERPSALQLLKHSFLERSH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001018047
ORF Size 1386 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001018047.1, NM_001018047.2, NP_001018057.1
RefSeq Size 1789 bp
RefSeq ORF 1389 bp
Locus ID 80122
Cytogenetics 2q21.3
Protein Families Druggable Genome, Protein Kinase
MW 53 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.