USP30 (NM_001301175) Human Tagged ORF Clone

CAT#: RC238509

USP30 (myc-DDK-tagged) - Human ubiquitin specific peptidase 30 (USP30), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238509 representing NM_001301175
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAACTGGGGAGTTATAGGTGGAATTGCTGCTGCTCTTGCAGCAGGAATATATGTTATTTGGGGTC
  CCATTACAGAAAGAAAGAAGCGTAGAAAAGGGCTTGTGCCTGGCCTTGTTAATTTAGGGAACACCTGCTT
  CATGAACTCCCTGCTACAAGGCCTGTCTGCCTGTCCTGCTTTCATCAGGTGGCTGGAAGAGTTCACCTCC
  CAGTACTCCAGGGATCAGAAGGAGCCCCCCTCACACCAGTATTTATCCTTAACACTCTTGCACCTTCTGA
  AAGCCTTGTCCTGCCAAGAAGTTACTGATGATGAGGTCTTAGATGCAAGCTGCTTGTTGGATGTCTTAAG
  AATGTACAGATGGCAGATCTCATCATTTGAAGAACAGGATGCTCACGAATTATTCCATGTCATTACCTCG
  TCATTGGAAGATGAGCGAGACCGCCAGCCTCGGGTCACACATTTGTTTGATGTGCATTCCCTGGAGCAGC
  AGTCAGAAATAACTCCCAAACAAATTACCTGCCGCACAAGAGGGTCACCTCACCCTACATCCAATCACTG
  GAAGTCTCAACATCCTTTTCATGGAAGACTCACTAGTAATATGGTCTGCAAACACTGTGAACACCAGAGT
  CCTGTTCGATTTGATACCTTTGATAGCCTTTCACTAAGTATTCCAGCCGCCACATGGGGTCACCCATTGA
  CCCTGGACCACTGCCTTCACCACTTCATCTCATCAGAATCAGTGCGGGATGTTGTGTGTGACAACTGTAC
  AAAGATTGAAGCCAAGGGAACGTTGAACGGGGAAAAGGTGGAACACCAGAGGACCACTTTTGTTAAACAG
  TTAAAACTAGGGAAGCTCCCTCAGTGTCTCTGCATCCACCTACAGCGGCTGAGCTGGTCCAGCCACGGCA
  CGCCTCTGAAGCGGCATGAGCACGTGCAGTTCAATGAGTTCCTGATGATGGACATTTACAAGTACCACCT
  CCTTGGACATAAACCTAGTCAACACAACCCTAAACTGAACAAGAACCCAGGGCCTACACTGGAGCTGCAG
  GATGGGCCGGGAGCCCCCACACCAGTTCTGAATCAGCCAGGGGCCCCCAAAACACAGATTTTTATGAATG
  GCGCCTGCTCCCCATCTTTATTGCCAACGCTGTCAGCGCCGATGCCCTTCCCTCTCCCAGTTGTTCCCGA
  CTACAGCTCCTCCACATACCTCTTCCGGCTGATGGCAGTTGTCGTCCACCATGGAGACATGCACTCTGGA
  CACTTTGTCACTTACCGACGGTCCCCACCTTCTGCCAGGAACCCTCTCTCAACTAGCAATCAGTGGCTGT
  GGGTCTCCGATGACACTGTCCGCAAGGCCAGCCTGCAGGAGGTCCTGTCCTCCAGCGCCTACCTGCTGTT
  CTACGAGCGCGTCCTTTCCAGGATGCAGCACCAGAGCCAGGAGTGCAAGTCTGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238509 representing NM_001301175
  Red=Cloning site Green=Tags(s)
  
  MKNWGVIGGIAAALAAGIYVIWGPITERKKRRKGLVPGLVNLGNTCFMNSLLQGLSACPAFIRWLEEFTS
  QYSRDQKEPPSHQYLSLTLLHLLKALSCQEVTDDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITS
  SLEDERDRQPRVTHLFDVHSLEQQSEITPKQITCRTRGSPHPTSNHWKSQHPFHGRLTSNMVCKHCEHQS
  PVRFDTFDSLSLSIPAATWGHPLTLDHCLHHFISSESVRDVVCDNCTKIEAKGTLNGEKVEHQRTTFVKQ
  LKLGKLPQCLCIHLQRLSWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPKLNKNPGPTLELQ
  DGPGAPTPVLNQPGAPKTQIFMNGACSPSLLPTLSAPMPFPLPVVPDYSSSTYLFRLMAVVVHHGDMHSG
  HFVTYRRSPPSARNPLSTSNQWLWVSDDTVRKASLQEVLSSSAYLLFYERVLSRMQHQSQECKSEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301175
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001301175.1, NP_001288104.1
• RefSeq Size: 4145
• RefSeq ORF: 1461
• Locus ID: 84749
• Protein Families: Protease, Transmembrane
• MW: 55.6 kDa
• Gene Summary: USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]). [supplied by OMIM, Dec 2008]