GRK4 (NM_005307) Human Tagged ORF Clone

CAT#: RC238584

GRK4 (myc-DDK-tagged) - Human G protein-coupled receptor kinase 4 (GRK4), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRK4
• Synonyms: GPRK2L; GPRK4; GRK4a; IT11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238584 representing NM_005307
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCGAGAACATCGTGGCCAACTCGCTGCTGCTGAAAGCGCGTCAAGAAAAGGATTATAGCAGTC
  TTTGTGACAAGCAACCGATAGGAAGACGTCTCTTCAGGCAGTTCTGTGATACCAAACCCACTCTAAAGAG
  GCACATTGAATTCTTGGATGCAGTGGCAGAATATGAAGTTGCCGATGATGAGGACCGAAGTGATTGTGGA
  CTGTCAATCTTAGATAGATTCTTCAATGATAAGTTGGCAGCCCCTTTACCAGAAATACCTCCAGATGTTG
  TGACAGAATGTAGATTGGGACTGAAGGAGGAGAACCCTTCCAAAAAAGCCTTTGAGGAATGTACTAGAGT
  TGCCCATAACTACCTAAGAGGGGAACCATTTGAAGAATACCAAGAAAGCTCATATTTTTCTCAGTTTTTA
  CAATGGAAATGGCTGGAAAGGCAACCCGTAACAAAGAACACATTTAGACATTACAGAGTTCTAGGAAAAG
  GCGGATTTGGAGAGGTTTGCGCCTGTCAAGTGCGAGCCACAGGAAAAATGTATGCCTGCAAAAAGCTACA
  AAAAAAAAGAATAAAGAAGAGGAAAGGTGAAGCTATGGCTCTAAATGAGAAAAGAATTCTGGAGAAAGTG
  CAAAGTAGATTCGTAGTTAGTTTAGCCTACGCTTATGAAACCAAAGATGCCTTGTGCTTGGTGCTCACCA
  TTATGAATGGAGGGGATTTGAAGTTTCACATTTACAACCTGGGCAATCCCGGCTTTGATGAGCAGAGAGC
  CGTTTTCTATGCTGCAGAGCTGTGTTGCGGCTTGGAAGATTTACAGAGGGAAAGAATTGTATACAGAGAC
  TTGAAGCCTGAGAATATTCTCCTTGATGATCGTGGACACATCCGGATTTCAGACCTCGGTTTGGCCACAG
  AGATCCCAGAAGGACAGAGGGTTCGAGGAAGAGTTGGAACAGTCGGCTACATGGCACCTGAAGTTGTCAA
  TAATGAAAAGTATACGTTTAGTCCCGATTGGTGGGGACTTGGCTGTCTGATCTATGAAATGATTCAGGGA
  CATTCTCCATTCAAAAAATACAAAGAGAAAGTCAAATGGGAGGAGGTCGATCAAAGAATCAAGAATGATA
  CCGAGGAGTATTCTGAGAAGTTTTCAGAGGATGCCAAATCTATCTGCAGGATGTTACTCACCAAGAATCC
  AAGCAAGCGGCTGGGCTGCAGGGGCGAGGGAGCGGCTGGGGTGAAGCAGCACCCCGTGTTCAAGGACATC
  AACTTCAGGAGGCTGGAGGCAAACATGCTGGAGCCCCCTTTCTGTCCTGATCCTCATGCCGTTTACTGTA
  AGGACGTCCTGGATATCGAGCAGTTCTCGGTGGTGAAAGGGATCTACCTGGACACCGCAGATGAAGACTT
  CTATGCTCGGTTTGCTACCGGGTGTGTCTCCATCCCCTGGCAGAATGAGGGCTGCCTGACCATGGTCCCC
  AGTGAGAAGGAAGTGGAACCCAAGCAATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238584 representing NM_005307
  Red=Cloning site Green=Tags(s)
  
  MELENIVANSLLLKARQEKDYSSLCDKQPIGRRLFRQFCDTKPTLKRHIEFLDAVAEYEVADDEDRSDCG
  LSILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSKKAFEECTRVAHNYLRGEPFEEYQESSYFSQFL
  QWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLQKKRIKKRKGEAMALNEKRILEKV
  QSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLGNPGFDEQRAVFYAAELCCGLEDLQRERIVYRD
  LKPENILLDDRGHIRISDLGLATEIPEGQRVRGRVGTVGYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQG
  HSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKSICRMLLTKNPSKRLGCRGEGAAGVKQHPVFKDI
  NFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSVVKGIYLDTADEDFYARFATGCVSIPWQNEGCLTMVP
  SEKEVEPKQC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005307
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005307.1, NM_005307.2, NP_005298.2
• RefSeq Size: 2087 bp
• RefSeq ORF: 1503 bp
• Locus ID: 2868
• Cytogenetics: 4p16.3
• Domains: RGS, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• MW: 58.1 kDa
• Gene Summary: 'This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]'