GRK4 (NM_005307) Human Untagged Clone

CAT#: SC336478

GRK4 (untagged) - Human G protein-coupled receptor kinase 4 (GRK4), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRK4
• Synonyms: GPRK2L; GPRK4; GRK4a; IT11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005307, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCTCGAGAACATCGTGGCCAACTCGCTGCTGCTGAAAGCGCGTCAAGAAAAGGATTATAGCAGTC
  TTTGTGACAAGCAACCGATAGGAAGACGTCTCTTCAGGCAGTTCTGTGATACCAAACCCACTCTAAAGAG
  GCACATTGAATTCTTGGATGCAGTGGCAGAATATGAAGTTGCCGATGATGAGGACCGAAGTGATTGTGGA
  CTGTCAATCTTAGATAGATTCTTCAATGATAAGTTGGCAGCCCCTTTACCAGAAATACCTCCAGATGTTG
  TGACAGAATGTAGATTGGGACTGAAGGAGGAGAACCCTTCCAAAAAAGCCTTTGAGGAATGTACTAGAGT
  TGCCCATAACTACCTAAGAGGGGAACCATTTGAAGAATACCAAGAAAGCTCATATTTTTCTCAGTTTTTA
  CAATGGAAATGGCTGGAAAGGCAACCCGTAACAAAGAACACATTTAGACATTACAGAGTTCTAGGAAAAG
  GCGGATTTGGAGAGGTTTGCGCCTGTCAAGTGCGAGCCACAGGAAAAATGTATGCCTGCAAAAAGCTACA
  AAAAAAAAGAATAAAGAAGAGGAAAGGTGAAGCTATGGCTCTAAATGAGAAAAGAATTCTGGAGAAAGTG
  CAAAGTAGATTCGTAGTTAGTTTAGCCTACGCTTATGAAACCAAAGATGCCTTGTGCTTGGTGCTCACCA
  TTATGAATGGAGGGGATTTGAAGTTTCACATTTACAACCTGGGCAATCCCGGCTTTGATGAGCAGAGAGC
  CGTTTTCTATGCTGCAGAGCTGTGTTGCGGCTTGGAAGATTTACAGAGGGAAAGAATTGTATACAGAGAC
  TTGAAGCCTGAGAATATTCTCCTTGATGATCGTGGACACATCCGGATTTCAGACCTCGGTTTGGCCACAG
  AGATCCCAGAAGGACAGAGGGTTCGAGGAAGAGTTGGAACAGTCGGCTACATGGCACCTGAAGTTGTCAA
  TAATGAAAAGTATACGTTTAGTCCCGATTGGTGGGGACTTGGCTGTCTGATCTATGAAATGATTCAGGGA
  CATTCTCCATTCAAAAAATACAAAGAGAAAGTCAAATGGGAGGAGGTCGATCAAAGAATCAAGAATGATA
  CCGAGGAGTATTCTGAGAAGTTTTCAGAGGATGCCAAATCTATCTGCAGGATGTTACTCACCAAGAATCC
  AAGCAAGCGGCTGGGCTGCAGGGGCGAGGGAGCGGCTGGGGTGAAGCAGCACCCCGTGTTCAAGGACATC
  AACTTCAGGAGGCTGGAGGCAAACATGCTGGAGCCCCCTTTCTGTCCTGATCCTCATGCCGTTTACTGTA
  AGGACGTCCTGGATATCGAGCAGTTCTCGGTGGTGAAAGGGATCTACCTGGACACCGCAGATGAAGACTT
  CTATGCTCGGTTTGCTACCGGGTGTGTCTCCATCCCCTGGCAGAATGAGGGCTGCCTGACCATGGTCCCC
  AGTGAGAAGGAAGTGGAACCCAAGCAATGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005307
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005307.2, NP_005298.2
• RefSeq Size: 2087 bp
• RefSeq ORF: 1503 bp
• Locus ID: 2868
• Cytogenetics: 4p16.3
• Domains: RGS, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• Gene Summary: 'This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]'
  Transcript Variant: This variant (4) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (delta) has the same N- and C-termini but is shorter compared to isoform alpha.